DAT11_ORYSJ
ID DAT11_ORYSJ Reviewed; 538 AA.
AC Q5I396; Q0DK40; Q688L6;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Diacylglycerol O-acyltransferase 1-1 {ECO:0000305};
DE Short=OsDGAT1-1 {ECO:0000305};
DE EC=2.3.1.20 {ECO:0000305};
GN Name=DGAT1-1 {ECO:0000305}; Synonyms=DAGAT {ECO:0000303|PubMed:16432735};
GN OrderedLocusNames=Os05g0196800 {ECO:0000312|EMBL:BAF16783.2},
GN LOC_Os05g10810 {ECO:0000305};
GN ORFNames=P0617H07.12 {ECO:0000312|EMBL:AAV59457.1},
GN P0636E04.4 {ECO:0000312|EMBL:AAU10815.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=16432735; DOI=10.1007/s00122-006-0210-9;
RA Wang H.W., Zhang J.S., Gai J.Y., Chen S.Y.;
RT "Cloning and comparative analysis of the gene encoding diacylglycerol
RT acyltransferase from wild type and cultivated soybean.";
RL Theor. Appl. Genet. 112:1086-1097(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Involved in triacylglycerol (TAG) synthesis. Catalyzes the
CC acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using
CC acyl-CoA. {ECO:0000250|UniProtKB:Q5GKZ7}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000305};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q5GKZ7}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family.
CC Sterol o-acyltransferase subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAU10815.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAV59457.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY858584; AAW47581.1; -; mRNA.
DR EMBL; AC132493; AAU10815.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC135427; AAV59457.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008211; BAF16783.2; -; Genomic_DNA.
DR EMBL; AP014961; BAS92683.1; -; Genomic_DNA.
DR RefSeq; XP_015639406.1; XM_015783920.1.
DR AlphaFoldDB; Q5I396; -.
DR SMR; Q5I396; -.
DR STRING; 4530.OS05T0196800-00; -.
DR PaxDb; Q5I396; -.
DR PRIDE; Q5I396; -.
DR EnsemblPlants; Os05t0196800-00; Os05t0196800-00; Os05g0196800.
DR GeneID; 4338045; -.
DR Gramene; Os05t0196800-00; Os05t0196800-00; Os05g0196800.
DR KEGG; osa:4338045; -.
DR eggNOG; KOG0380; Eukaryota.
DR HOGENOM; CLU_018190_0_1_1; -.
DR InParanoid; Q5I396; -.
DR OMA; VPNVYLW; -.
DR OrthoDB; 1275897at2759; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0009941; C:chloroplast envelope; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; ISS:UniProtKB.
DR GO; GO:0008374; F:O-acyltransferase activity; IBA:GO_Central.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; ISS:UniProtKB.
DR InterPro; IPR027251; Diacylglycerol_acylTrfase1.
DR InterPro; IPR004299; MBOAT_fam.
DR InterPro; IPR014371; Oat_ACAT_DAG_ARE.
DR PANTHER; PTHR10408; PTHR10408; 1.
DR Pfam; PF03062; MBOAT; 1.
DR PIRSF; PIRSF000439; Oat_ACAT_DAG_ARE; 1.
DR PIRSF; PIRSF500231; Oat_dag; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Endoplasmic reticulum; Glycerol metabolism;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..538
FT /note="Diacylglycerol O-acyltransferase 1-1"
FT /id="PRO_0000438909"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 451..471
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 474..494
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 505..525
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 54..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 409..415
FT /note="FYXDWWN motif"
FT /evidence="ECO:0000250|UniProtKB:O75907"
FT COMPBIAS 71..88
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 464
FT /evidence="ECO:0000250|UniProtKB:O75907"
SQ SEQUENCE 538 AA; 59604 MW; 280E28B80E758602 CRC64;
MVGSDGDGDG GGGEAHAPAA PAHHHRRPPR PRGGSGAIVE GFAAALRRRI RSGAAAAARA
SFGGDSGDEA ASGEPSSSSS SSPSRRRGGD SNGAEASSAA GGGGGRGGGG DFSAFTFRAA
APVHRKAKES PLSSDAIFKQ SHAGLFNLCI VVLVAVNSRL IIENLMKYGL LIRAGFWFND
KSLRDWPLLM CCLSLPAFPL GAFAVEKLAF NNVITDAVAT CLHIFLSTTE IVYPVLVILK
CDSAVLSGFL LIFIACIVWL KLVSFAHTNH DIRQLTMGGK KVDNELSTVD MDNLQPPTLG
NLIYFMMAPT LCYQPSYPRT SCVRKGWLIR QIILYLIFTG LQGFIIEQYI NPIVVNSQHP
LKGGLLNAVE TVLKLSLPNV YLWLCMFYAF FHLWLSILAE ILRFGDREFY KDWWNAKTID
EYWRKWNMPV HKWVVRHIYF PCMRNGISKE VAVLISFLVS AVLHEICVAV PCRILKFWAF
LGIMLQIPLI VLTAYLKSKF RDTMVGNMIF WFFFCIYGQP MCLLLYYHDV MNRIEKAR
