DAT12_ORYSJ
ID DAT12_ORYSJ Reviewed; 477 AA.
AC A0A0P0WY03; Q0DBH9; Q5Z8Z2;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2016, sequence version 1.
DT 25-MAY-2022, entry version 41.
DE RecName: Full=Diacylglycerol O-acyltransferase 1-2 {ECO:0000305};
DE Short=OsDGAT1-2 {ECO:0000305};
DE EC=2.3.1.20 {ECO:0000305};
GN Name=DGAT1-2 {ECO:0000305};
GN OrderedLocusNames=Os06g0563900 {ECO:0000312|EMBL:BAS98267.1},
GN LOC_Os06g36800 {ECO:0000305};
GN ORFNames=P0656E03.36 {ECO:0000312|EMBL:BAD53762.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Involved in triacylglycerol (TAG) synthesis. Catalyzes the
CC acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using
CC acyl-CoA. {ECO:0000250|UniProtKB:Q5GKZ7}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000305};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q5GKZ7}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family.
CC Sterol o-acyltransferase subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD53762.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF19794.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP003714; BAD53762.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008212; BAF19794.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014962; BAS98267.1; -; Genomic_DNA.
DR RefSeq; XP_015641041.1; XM_015785555.1.
DR AlphaFoldDB; A0A0P0WY03; -.
DR SMR; A0A0P0WY03; -.
DR STRING; 4530.OS06T0563900-01; -.
DR PaxDb; A0A0P0WY03; -.
DR EnsemblPlants; Os06t0563900-01; Os06t0563900-01; Os06g0563900.
DR GeneID; 4341317; -.
DR Gramene; Os06t0563900-01; Os06t0563900-01; Os06g0563900.
DR KEGG; osa:4341317; -.
DR eggNOG; KOG0380; Eukaryota.
DR OMA; HAIIVWL; -.
DR OrthoDB; 1275897at2759; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR GO; GO:0009941; C:chloroplast envelope; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; ISS:UniProtKB.
DR GO; GO:0008374; F:O-acyltransferase activity; IBA:GO_Central.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; ISS:UniProtKB.
DR InterPro; IPR027251; Diacylglycerol_acylTrfase1.
DR InterPro; IPR004299; MBOAT_fam.
DR InterPro; IPR014371; Oat_ACAT_DAG_ARE.
DR PANTHER; PTHR10408; PTHR10408; 1.
DR Pfam; PF03062; MBOAT; 1.
DR PIRSF; PIRSF000439; Oat_ACAT_DAG_ARE; 1.
DR PIRSF; PIRSF500231; Oat_dag; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Endoplasmic reticulum; Glycerol metabolism;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..477
FT /note="Diacylglycerol O-acyltransferase 1-2"
FT /id="PRO_0000438910"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 263..283
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 346..352
FT /note="FYXDWWN motif"
FT /evidence="ECO:0000250|UniProtKB:O75907"
FT ACT_SITE 401
FT /evidence="ECO:0000250|UniProtKB:O75907"
SQ SEQUENCE 477 AA; 55108 MW; 476B1B5BBDD044A2 CRC64;
MAPPPSLAPD RGGGEPDDAL RLRARAAAAA GDAPAPQQQQ EQRHQEQQQQ LLWYRASAPA
HRRVRESPLS SDAIFRQSHA GLLNLCIVVL VAVNSRLIIE NLMKYGLLIR AGFWFSGTSL
ADWPLLMCCL TLPTFPLAAL MVEKLAQRKL ISKHVVILLH IVITTSVLVY PVVVILKCDS
AVLSGFVLMF LASIIWLKLV SFAHTNYDIR MLSKSIEKGV THDISIDPEN IKWPTFKRLS
YFMLAPTLCY QPSYPRTTYI RKGWVVRQLI KCLVFTGLMG FIIEQYINPI VKNSKHPLKG
NFLNAIERVL KLSVPTLYVW LCMFYCFFHL WLNILAELLC FGDREFYKDW WNAKTVEEYW
RMWNMPVHKW VIRHIYFPCI RNGFSKGVAI LISFLVSAAF HELCVAVPCH IFKFWAFIGI
MFQIPLVFLT KYLQDKFNNT MVGNMIFWFF FSILGQPMCV LLYYHDVMNR QQAQTNR
