DAT1B_SOYBN
ID DAT1B_SOYBN Reviewed; 504 AA.
AC I1MSF2; Q1MW30;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=Diacylglycerol O-acyltransferase 1B {ECO:0000305};
DE Short=GmDGAT1B {ECO:0000303|PubMed:23322364};
DE EC=2.3.1.20 {ECO:0000269|PubMed:23322364};
GN Name=DGAT1B {ECO:0000303|PubMed:23322364};
GN OrderedLocusNames=Glyma17g053300 {ECO:0000312|EMBL:KRH02683.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Jack;
RX PubMed=23322364; DOI=10.1007/s10142-012-0306-z;
RA Li R., Hatanaka T., Yu K., Wu Y., Fukushige H., Hildebrand D.;
RT "Soybean oil biosynthesis: role of diacylglycerol acyltransferases.";
RL Funct. Integr. Genomics 13:99-113(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82;
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
CC -!- FUNCTION: Major contributors to triacylglycerol (TAG) synthesis and oil
CC accumulation in developing seeds. Catalyzes the acylation of the sn-3
CC hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Has a marked
CC preference for oleoyl-CoA and sn-1,2-dioleoylglycerol over vernoloyl-
CC CoA and sn-1,2-divernoloylglycerol. {ECO:0000269|PubMed:23322364}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000269|PubMed:23322364};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q5GKZ7}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Highly expressed in flowers and pods. Expressed at
CC low levels in roots, stems and leaves. {ECO:0000269|PubMed:23322364}.
CC -!- DEVELOPMENTAL STAGE: During seed development, expressed from 30 to 65
CC days after flowering (DAF), with a peak at 50 DAF.
CC {ECO:0000269|PubMed:23322364}.
CC -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family.
CC Sterol o-acyltransferase subfamily. {ECO:0000305}.
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DR EMBL; AB257590; BAE93461.1; -; mRNA.
DR EMBL; CM000850; KRH02683.1; -; Genomic_DNA.
DR RefSeq; NP_001237684.1; NM_001250755.1.
DR AlphaFoldDB; I1MSF2; -.
DR SMR; I1MSF2; -.
DR STRING; 3847.GLYMA17G06120.1; -.
DR PRIDE; I1MSF2; -.
DR EnsemblPlants; KRH02683; KRH02683; GLYMA_17G053300.
DR GeneID; 732606; -.
DR Gramene; KRH02683; KRH02683; GLYMA_17G053300.
DR KEGG; gmx:732606; -.
DR eggNOG; KOG0380; Eukaryota.
DR HOGENOM; CLU_018190_0_1_1; -.
DR InParanoid; I1MSF2; -.
DR OMA; TMRVEHL; -.
DR OrthoDB; 1275897at2759; -.
DR BRENDA; 2.3.1.20; 2483.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000008827; Chromosome 17.
DR GO; GO:0009941; C:chloroplast envelope; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IDA:UniProtKB.
DR GO; GO:0008374; F:O-acyltransferase activity; IBA:GO_Central.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IDA:UniProtKB.
DR InterPro; IPR027251; Diacylglycerol_acylTrfase1.
DR InterPro; IPR004299; MBOAT_fam.
DR InterPro; IPR014371; Oat_ACAT_DAG_ARE.
DR PANTHER; PTHR10408; PTHR10408; 1.
DR Pfam; PF03062; MBOAT; 1.
DR PIRSF; PIRSF000439; Oat_ACAT_DAG_ARE; 1.
DR PIRSF; PIRSF500231; Oat_dag; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Endoplasmic reticulum; Glycerol metabolism;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..504
FT /note="Diacylglycerol O-acyltransferase 1B"
FT /id="PRO_0000438906"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..458
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 471..491
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 375..381
FT /note="FYXDWWN motif"
FT /evidence="ECO:0000250|UniProtKB:O75907"
FT COMPBIAS 10..41
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..72
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 430
FT /evidence="ECO:0000250|UniProtKB:O75907"
FT CONFLICT 406
FT /note="F -> Y (in Ref. 1; BAE93461)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 504 AA; 57999 MW; EBA63F49A0B9BA49 CRC64;
MAISDEPESV ATALNHSSLR RRPSATSTAG LFNSPETTTD SSGDDLAKDS GSDDSINSDD
AAVNSQQQNE KQDTDFSVLK FAYRPSVPAH RKVKESPLSS DTIFRQSHAG LFNLCIVVLV
AVNSRLIIEN LMKYGWLIKS GFWFSSKSLR DWPLFMCCLS LVVFPFAAFI VEKLAQRKCI
PEPVVVVLHI IITSTSLFYP VLVILRCDSA FVSGVTLMLF SCVVWLKLVS YAHTNYDMRA
LTKLVEKGEA LLDTLNMDYP YNVSFKSLAY FLVAPTLCYQ PSYPRTPYIR KGWLFRQLVK
LIIFTGVMGF IIEQYINPIV QNSQHPLKGN LLYATERVLK LSVPNLYVWL CMFYCFFHLW
LNILAELLRF GDREFYKDWW NAKTVEDYWR MWNMPVHKWM IRHLYFPCLR HGLPKAAALL
IAFLVSALFH ELCIAVPCHI FKLWAFGGIM FQVPLVLITN YLQNKFRNSM VGNMIFWFIF
SILGQPMCVL LYYHDLMNRK GKLD
