ID   DATIN_MYCTU             Reviewed;         273 AA.
AC   P9WMA9; L0T2D8; O53624; Q7DAH6;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=Dormancy associated translation inhibitor {ECO:0000303|PubMed:22719925};
DE            Short=DATIN {ECO:0000303|PubMed:22719925};
GN   OrderedLocusNames=Rv0079;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   INDUCTION BY HYPOXIA.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=11416222; DOI=10.1073/pnas.121172498;
RA   Sherman D.R., Voskuil M., Schnappinger D., Liao R., Harrell M.I.,
RA   Schoolnik G.K.;
RT   "Regulation of the Mycobacterium tuberculosis hypoxic response gene
RT   encoding alpha -crystallin.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7534-7539(2001).
RN   [3]
RP   INDUCTION BY NITRIC OXIDE (NO) AND BY HYPOXIA, AND DORMANCY REGULON.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=12953092; DOI=10.1084/jem.20030205;
RA   Voskuil M.I., Schnappinger D., Visconti K.C., Harrell M.I., Dolganov G.M.,
RA   Sherman D.R., Schoolnik G.K.;
RT   "Inhibition of respiration by nitric oxide induces a Mycobacterium
RT   tuberculosis dormancy program.";
RL   J. Exp. Med. 198:705-713(2003).
RN   [4]
RP   INDUCTION BY CARBON MONOXIDE (CO).
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=18474359; DOI=10.1016/j.chom.2008.03.007;
RA   Shiloh M.U., Manzanillo P., Cox J.S.;
RT   "Mycobacterium tuberculosis senses host-derived carbon monoxide during
RT   macrophage infection.";
RL   Cell Host Microbe 3:323-330(2008).
RN   [5]
RP   INDUCTION BY CARBON MONOXIDE (CO), AND DORMANCY REGULON.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=18400743; DOI=10.1074/jbc.m802274200;
RA   Kumar A., Deshane J.S., Crossman D.K., Bolisetty S., Yan B.S., Kramnik I.,
RA   Agarwal A., Steyn A.J.;
RT   "Heme oxygenase-1-derived carbon monoxide induces the Mycobacterium
RT   tuberculosis dormancy regulon.";
RL   J. Biol. Chem. 283:18032-18039(2008).
RN   [6]
RP   FUNCTION PREDICTION.
RX   PubMed=19795912; DOI=10.1080/07391102.2009.10507316;
RA   Mishra S.;
RT   "Function prediction of Rv0079, a hypothetical Mycobacterium tuberculosis
RT   DosR regulon protein.";
RL   J. Biomol. Struct. Dyn. 27:283-292(2009).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [8]
RP   FUNCTION IN E.COLI, AND SUBUNIT.
RX   PubMed=22719925; DOI=10.1371/journal.pone.0038709;
RA   Kumar A., Majid M., Kunisch R., Rani P.S., Qureshi I.A., Lewin A.,
RA   Hasnain S.E., Ahmed N.;
RT   "Mycobacterium tuberculosis DosR regulon gene Rv0079 encodes a putative,
RT   'dormancy associated translation inhibitor (DATIN)'.";
RL   PLoS ONE 7:E38709-E38709(2012).
RN   [9]
RP   FUNCTION, AND INTERACTION WITH TLR2.
RX   PubMed=23819907; DOI=10.1016/j.cyto.2013.06.310;
RA   Kumar A., Lewin A., Rani P.S., Qureshi I.A., Devi S., Majid M., Kamal E.,
RA   Marek S., Hasnain S.E., Ahmed N.;
RT   "Dormancy associated translation inhibitor (DATIN/Rv0079) of Mycobacterium
RT   tuberculosis interacts with TLR2 and induces proinflammatory cytokine
RT   expression.";
RL   Cytokine 64:258-264(2013).
CC   -!- FUNCTION: Involved in translation regulation (PubMed:19795912,
CC       PubMed:22719925). Inhibits protein synthesis and decreases bacterial
CC       growth when expressed in E.coli (PubMed:22719925). Can also stimulate
CC       macrophages and peripheral blood mononuclear cells (PBMC) to secrete
CC       important cytokines that may be significant in granuloma formation and
CC       its maintenance. Increases secretion of IFN-gamma, TNF-alpha, IL-1 beta
CC       and IL-8 through human Toll-like receptor 2 (TLR2) signaling pathway
CC       (PubMed:23819907). {ECO:0000269|PubMed:22719925,
CC       ECO:0000269|PubMed:23819907, ECO:0000305|PubMed:19795912}.
CC   -!- SUBUNIT: Interacts with human TLR2 (PubMed:23819907). Monomer in
CC       solution (PubMed:22719925). In vitro, binds to E.coli ribosomes
CC       (PubMed:22719925). {ECO:0000269|PubMed:22719925,
CC       ECO:0000269|PubMed:23819907}.
CC   -!- INDUCTION: A member of the dormancy regulon. Induced in response to
CC       reduced oxygen tension (hypoxia), low levels of nitric oxide (NO) and
CC       carbon monoxide (CO). It is hoped that this regulon will give insight
CC       into the latent, or dormant phase of infection.
CC       {ECO:0000269|PubMed:11416222, ECO:0000269|PubMed:12953092,
CC       ECO:0000269|PubMed:18400743, ECO:0000269|PubMed:18474359}.
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DR   EMBL; AL123456; CCP42804.1; -; Genomic_DNA.
DR   PIR; H70849; H70849.
DR   RefSeq; NP_214593.1; NC_000962.3.
DR   RefSeq; WP_003400655.1; NZ_NVQJ01000005.1.
DR   AlphaFoldDB; P9WMA9; -.
DR   STRING; 83332.Rv0079; -.
DR   PaxDb; P9WMA9; -.
DR   DNASU; 886995; -.
DR   GeneID; 886995; -.
DR   KEGG; mtu:Rv0079; -.
DR   TubercuList; Rv0079; -.
DR   eggNOG; COG1544; Bacteria.
DR   OMA; RPRPWPD; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0043024; F:ribosomal small subunit binding; IBA:GO_Central.
DR   GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR   GO; GO:0045900; P:negative regulation of translational elongation; IBA:GO_Central.
DR   Gene3D; 3.30.505.50; -; 2.
DR   InterPro; IPR032528; Ribosom_S30AE_C.
DR   InterPro; IPR038416; Ribosom_S30AE_C_sf.
DR   Pfam; PF16321; Ribosom_S30AE_C; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Virulence.
FT   CHAIN           1..273
FT                   /note="Dormancy associated translation inhibitor"
FT                   /id="PRO_0000392679"
SQ   SEQUENCE   273 AA;  29507 MW;  07F5BDC73A403E2B CRC64;
     MEPKRSRLVV CAPEPSHARE FPDVAVFSGG RANASQAERL ARAVGRVLAD RGVTGGARVR
     LTMANCADGP TLVQINLQVG DTPLRAQAAT AGIDDLRPAL IRLDRQIVRA SAQWCPRPWP
     DRPRRRLTTP AEALVTRRKP VVLRRATPLQ AIAAMDAMDY DVHLFTDAET GEDAVVYRAG
     PSGLRLARQH HVFPPGWSRC RAPAGPPVPL IVNSRPTPVL TEAAAVDRAR EHGLPFLFFT
     DQATGRGQLL YSRYDGNLGL ITPTGDGVAD GLA