DATI_YEAST
ID DATI_YEAST Reviewed; 248 AA.
AC P13483; D6W0H1;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Oligo(A)/oligo(T)-binding protein;
DE AltName: Full=Datin;
GN Name=DAT1; Synonyms=DAT; OrderedLocusNames=YML113W; ORFNames=YM8339.06;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2670564; DOI=10.1002/j.1460-2075.1989.tb03583.x;
RA Winter E., Varshavsky A.;
RT "A DNA binding protein that recognizes oligo(dA).oligo(dT) tracts.";
RL EMBO J. 8:1867-1877(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP CHARACTERIZATION.
RX PubMed=8532535; DOI=10.1093/nar/23.23.4900;
RA Reardon B.J., Gordon D., Ballard M.J., Winter E.;
RT "DNA binding properties of the Saccharomyces cerevisiae DAT1 gene
RT product.";
RL Nucleic Acids Res. 23:4900-4906(1995).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: DNA-binding protein that recognizes oligo(A).oligo(T) tracts
CC (A.T DNA). Can bind to any 11 bp sequence in which 10 bases conform to
CC an uninterrupted oligo(A).oligo(T) tract.
CC -!- SUBUNIT: Binds as a dimer or higher oligomer.
CC -!- MISCELLANEOUS: Present with 4700 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X15478; CAA33505.1; -; Genomic_DNA.
DR EMBL; Z49210; CAA89105.1; -; Genomic_DNA.
DR EMBL; AY557765; AAS56091.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09785.1; -; Genomic_DNA.
DR PIR; S22853; S22853.
DR RefSeq; NP_013594.1; NM_001182475.1.
DR AlphaFoldDB; P13483; -.
DR SMR; P13483; -.
DR BioGRID; 35091; 92.
DR STRING; 4932.YML113W; -.
DR iPTMnet; P13483; -.
DR MaxQB; P13483; -.
DR PaxDb; P13483; -.
DR PRIDE; P13483; -.
DR EnsemblFungi; YML113W_mRNA; YML113W; YML113W.
DR GeneID; 854927; -.
DR KEGG; sce:YML113W; -.
DR SGD; S000004581; DAT1.
DR VEuPathDB; FungiDB:YML113W; -.
DR eggNOG; ENOG502SC3B; Eukaryota.
DR HOGENOM; CLU_1134320_0_0_1; -.
DR InParanoid; P13483; -.
DR OMA; TXNGSNQ; -.
DR BioCyc; YEAST:G3O-32695-MON; -.
DR PRO; PR:P13483; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P13483; protein.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0003680; F:minor groove of adenine-thymine-rich DNA binding; IDA:SGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:SGD.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Repeat.
FT CHAIN 1..248
FT /note="Oligo(A)/oligo(T)-binding protein"
FT /id="PRO_0000079787"
FT REPEAT 8..12
FT /note="1"
FT REPEAT 14..18
FT /note="2"
FT REPEAT 26..30
FT /note="3"
FT DNA_BIND 1..36
FT REGION 1..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 8..30
FT /note="3 X 5 AA repeats of G-R-K-P-G"
FT REGION 219..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..67
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..127
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 248 AA; 27067 MW; D0E1E31C0C42A443 CRC64;
MAKTLAQGRK PGSGRKPGKG KTLREGRKPG SGRRRRQDTG GKETDGSQQD QESRLISSRD
MEAVDALREL THSPSSHSAH NSSAAPPPHA AAASTSLPPS LDYTHQSFMD QQQQQQQQQQ
QQLLQQQRVD VVPPKPFITH KILLSSTGNS GGHVNSNYNA DHSINHNSNH NLNSNVNVNM
NFTINGSNQD PSSSFLMGPY NYLQRPFIVK PYLDLSTSTA ASNQPRTQPS PAAHITKNSD
STEKNATI
