DAUA_ECO57
ID DAUA_ECO57 Reviewed; 559 AA.
AC P0AFR3; P40877; P76019;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=C4-dicarboxylic acid transporter DauA {ECO:0000250|UniProtKB:P0AFR2};
DE AltName: Full=Dicarboxylic acid uptake system A {ECO:0000250|UniProtKB:P0AFR2};
GN Name=dauA; OrderedLocusNames=Z1977, ECs1711;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Responsible for the aerobic transport of succinate from the
CC periplasm to the cytoplasm at acidic pH.
CC {ECO:0000250|UniProtKB:P0AFR2}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+)(in) + succinate(in) = 2 H(+)(out) + succinate(out);
CC Xref=Rhea:RHEA:29303, ChEBI:CHEBI:15378, ChEBI:CHEBI:30031;
CC Evidence={ECO:0000250|UniProtKB:P0AFR2};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P0AFR2}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG56064.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB35134.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE005174; AAG56064.1; ALT_INIT; Genomic_DNA.
DR EMBL; BA000007; BAB35134.1; ALT_INIT; Genomic_DNA.
DR PIR; D85700; D85700.
DR PIR; G90842; G90842.
DR RefSeq; NP_309738.2; NC_002695.1.
DR RefSeq; WP_001033352.1; NZ_SDVX01000011.1.
DR AlphaFoldDB; P0AFR3; -.
DR SMR; P0AFR3; -.
DR STRING; 155864.EDL933_1910; -.
DR EnsemblBacteria; AAG56064; AAG56064; Z1977.
DR EnsemblBacteria; BAB35134; BAB35134; ECs_1711.
DR GeneID; 66674974; -.
DR GeneID; 913152; -.
DR KEGG; ece:Z1977; -.
DR KEGG; ecs:ECs_1711; -.
DR PATRIC; fig|386585.9.peg.1809; -.
DR eggNOG; COG0659; Bacteria.
DR HOGENOM; CLU_003182_13_1_6; -.
DR OMA; VTNKFPI; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008271; F:secondary active sulfate transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 3.30.750.24; -; 1.
DR InterPro; IPR018045; S04_transporter_CS.
DR InterPro; IPR011547; SLC26A/SulP_dom.
DR InterPro; IPR001902; SLC26A/SulP_fam.
DR InterPro; IPR002645; STAS_dom.
DR InterPro; IPR036513; STAS_dom_sf.
DR PANTHER; PTHR11814; PTHR11814; 1.
DR Pfam; PF01740; STAS; 1.
DR Pfam; PF00916; Sulfate_transp; 1.
DR SUPFAM; SSF52091; SSF52091; 1.
DR TIGRFAMs; TIGR00815; sulP; 1.
DR PROSITE; PS01130; SLC26A; 1.
DR PROSITE; PS50801; STAS; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..559
FT /note="C4-dicarboxylic acid transporter DauA"
FT /id="PRO_0000080196"
FT TOPO_DOM 1..32
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..101
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..126
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..177
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..212
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 213..233
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..278
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..313
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 335..350
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 394..418
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 440..559
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AFR2"
FT DOMAIN 449..559
FT /note="STAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
SQ SEQUENCE 559 AA; 59429 MW; 14EC83860002F23C CRC64;
MNKIFSSHVM PFRALIDACW KEKYTAARFT RDLIAGITVG IIAIPLAMAL AIGSGVAPQY
GLYTAAVAGI VIALTGGSRF SVSGPTAAFV VILYPVSQQF GLAGLLVATL LSGIFLILMG
LARFGRLIEY IPVSVTLGFT SGIGITIGTM QIKDFLGLQM AHVPEHYLQK VGALFMALPT
INVGDAAIGI VTLGILVFWP RLGIRLPGHL PALLAGCAVM GIVNLLGGHV ATIGSQFHYV
LADGSQGNGI PQLLPQLVLP WDLPNSEFTL TWDSIRTLLP AAFSMAMLGA IESLLCAVVL
DGMTGTKHKA NSELVGQGLG NIIAPFFGGI TATAAIARSA ANVRAGATSP ISAVIHSILV
ILALLVLAPL LSWLPLSAMA ALLLMVAWNM SEAHKVVDLL RHAPKDDIIV MLLCMSLTVL
FDMVIAISVG IVLASLLFMR RIARMTRLAP VVVDVPDDVL VLRVIGPLFF AAAEGLFTDL
ESRLEGKRIV ILKWDAVPVL DAGGLDAFQR FVKRLPEGCE LRVCNVEFQP LRTMARAGIQ
PIPGRLAFFP NRRAAMADL
