DAW1_CHLRE
ID DAW1_CHLRE Reviewed; 446 AA.
AC Q3Y8L7;
DT 16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 1.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Dynein assembly factor with WDR repeat domains 1;
DE AltName: Full=Outer row dynein assembly protein 16;
GN Name=DAW1; Synonyms=ODA16;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RX PubMed=16093345; DOI=10.1091/mbc.e05-07-0627;
RA Ahmed N.T., Mitchell D.R.;
RT "ODA16p, a Chlamydomonas flagellar protein needed for dynein assembly.";
RL Mol. Biol. Cell 16:5004-5012(2005).
RN [2]
RP FUNCTION, INTERACTION WITH IFT46 AND FLA10, AND SUBCELLULAR LOCATION.
RX PubMed=18852297; DOI=10.1083/jcb.200802025;
RA Ahmed N.T., Gao C., Lucker B.F., Cole D.G., Mitchell D.R.;
RT "ODA16 aids axonemal outer row dynein assembly through an interaction with
RT the intraflagellar transport machinery.";
RL J. Cell Biol. 183:313-322(2008).
RN [3]
RP NOMENCLATURE.
RX PubMed=21953912; DOI=10.1002/cm.20533;
RA Hom E.F., Witman G.B., Harris E.H., Dutcher S.K., Kamiya R., Mitchell D.R.,
RA Pazour G.J., Porter M.E., Sale W.S., Wirschell M., Yagi T., King S.M.;
RT "A unified taxonomy for ciliary dyneins.";
RL Cytoskeleton 68:555-565(2011).
CC -!- FUNCTION: Functions as a cargo-specific adapter between intraflagellar
CC transport (IFT) particles and outer row dynein. Required for efficient
CC dynein-mediated transport into the flagellar compartment.
CC {ECO:0000269|PubMed:18852297}.
CC -!- SUBUNIT: Interacts with IFT46 and FLA10. {ECO:0000269|PubMed:18852297}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum basal body.
CC Cytoplasm, cytoskeleton, flagellum axoneme. Note=Expression is
CC concentrated at the flagellum basal body but is also detected along the
CC length of the flagellum.
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DR EMBL; DQ151642; AAZ77789.1; -; mRNA.
DR PDB; 5MZH; X-ray; 2.40 A; A/B=1-446.
DR PDBsum; 5MZH; -.
DR AlphaFoldDB; Q3Y8L7; -.
DR SMR; Q3Y8L7; -.
DR PRIDE; Q3Y8L7; -.
DR GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-KW.
DR GO; GO:0042073; P:intraciliary transport; IMP:UniProtKB.
DR GO; GO:0036158; P:outer dynein arm assembly; IMP:UniProtKB.
DR Gene3D; 2.130.10.10; -; 4.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 8.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 8.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 5.
DR PROSITE; PS50082; WD_REPEATS_2; 8.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum;
KW Repeat; WD repeat.
FT CHAIN 1..446
FT /note="Dynein assembly factor with WDR repeat domains 1"
FT /id="PRO_0000413906"
FT REPEAT 92..131
FT /note="WD 1"
FT REPEAT 134..174
FT /note="WD 2"
FT REPEAT 177..216
FT /note="WD 3"
FT REPEAT 219..260
FT /note="WD 4"
FT REPEAT 262..300
FT /note="WD 5"
FT REPEAT 303..342
FT /note="WD 6"
FT REPEAT 345..386
FT /note="WD 7"
FT REPEAT 388..426
FT /note="WD 8"
FT REGION 423..446
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 5..11
FT /evidence="ECO:0007829|PDB:5MZH"
FT TURN 12..15
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 16..21
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 29..33
FT /evidence="ECO:0007829|PDB:5MZH"
FT HELIX 43..53
FT /evidence="ECO:0007829|PDB:5MZH"
FT HELIX 59..61
FT /evidence="ECO:0007829|PDB:5MZH"
FT HELIX 62..76
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 85..91
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 97..102
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 104..113
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 118..122
FT /evidence="ECO:0007829|PDB:5MZH"
FT TURN 123..125
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 128..132
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 139..144
FT /evidence="ECO:0007829|PDB:5MZH"
FT TURN 146..148
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 150..156
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 161..165
FT /evidence="ECO:0007829|PDB:5MZH"
FT TURN 166..168
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 171..175
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 182..187
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 191..198
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 203..207
FT /evidence="ECO:0007829|PDB:5MZH"
FT TURN 208..210
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 213..217
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 224..226
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 233..240
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 245..249
FT /evidence="ECO:0007829|PDB:5MZH"
FT TURN 250..252
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 255..259
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 266..271
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 275..282
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 287..291
FT /evidence="ECO:0007829|PDB:5MZH"
FT TURN 292..294
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 297..301
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 308..313
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 315..324
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 329..333
FT /evidence="ECO:0007829|PDB:5MZH"
FT TURN 334..336
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 339..343
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 350..355
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 359..366
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 369..375
FT /evidence="ECO:0007829|PDB:5MZH"
FT TURN 376..378
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 381..386
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 392..397
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 399..408
FT /evidence="ECO:0007829|PDB:5MZH"
FT STRAND 411..417
FT /evidence="ECO:0007829|PDB:5MZH"
SQ SEQUENCE 446 AA; 49250 MW; 06A9F12E71500453 CRC64;
MAAKLRKFLL RYYPPGIILQ YERDGVMKQK PIDLLDLTPD VDVEVLLSQI IRQEPLISEN
RRPALRQLIH RLIDKMLEQQ HHSFTLFKVL RAHILPLTNC AFNKSGDRFI TGSYDRTCKV
WNTFTGEEVF TLEGHKNVVY AIAFNNPYGD KIVTGSFDKT CKLWDAYTGQ LYYTLKGHQT
EIVCLSFNPQ STIIATGSMD NTAKLWDVET GQERATLAGH RAEIVSLGFN TGGDLIVTGS
FDHDSRLWDV RTGQCVHVLS GHRGEVSSTQ FNYAGTLVVS GSIDCTSRLW DVRSGRCLSV
KQGHTDEVLD VAFDAAGTKM VSASADGSAR LYHTLTGVCQ HTLVGHEGEI SKVAFNPQGT
RLITASSDKT CRLWDCDTGE CLQVLEGHTD EIFSCAFNYE GDFIITGSKD NTCRIWKALT
ASSQPAGPGG AGGGLARPTT AFSYND
