ID   DAW1_DANRE              Reviewed;         415 AA.
AC   Q1LV15; Q05AK4; Q05AM0;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Dynein assembly factor with WDR repeat domains 1;
DE   AltName: Full=Outer row dynein assembly protein 16 homolog;
DE   AltName: Full=WD repeat-containing protein 69;
GN   Name=daw1; Synonyms=oda16, wdr69; ORFNames=si:dkey-223n17.5, zgc:153626;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Olfactory epithelium, and Testis;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=20568242; DOI=10.1002/dvdy.22355;
RA   Gao C., Wang G., Amack J.D., Mitchell D.R.;
RT   "Oda16/Wdr69 is essential for axonemal dynein assembly and ciliary motility
RT   during zebrafish embryogenesis.";
RL   Dev. Dyn. 239:2190-2197(2010).
CC   -!- FUNCTION: Required for axonemal dynein assembly and ciliary motility in
CC       ciliated organs, including Kupffer's vesicle, during embryogenesis.
CC       {ECO:0000269|PubMed:20568242}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in organs bearing motile cilia, including
CC       the pronephros, otic vesicles and Kupffer's vesicle.
CC   -!- DEVELOPMENTAL STAGE: Detected at several stages during embryogenesis,
CC       but only within organs bearing motile cilia.
CC   -!- DISRUPTION PHENOTYPE: Defects are the cause of a phenotype related to
CC       primary ciliary dyskinesia (PCD). Embryos have curly tails, pronephric
CC       cysts and left-right asymmetry defects. {ECO:0000269|PubMed:20568242}.
CC   -!- SIMILARITY: Belongs to the WD repeat WDR69 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAK05479.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; BX908751; CAK05479.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BC124403; AAI24404.1; -; mRNA.
DR   EMBL; BC124455; AAI24456.1; -; mRNA.
DR   AlphaFoldDB; Q1LV15; -.
DR   SMR; Q1LV15; -.
DR   STRING; 7955.ENSDARP00000116595; -.
DR   PaxDb; Q1LV15; -.
DR   PRIDE; Q1LV15; -.
DR   ZFIN; ZDB-GENE-050419-255; daw1.
DR   eggNOG; KOG0272; Eukaryota.
DR   InParanoid; Q1LV15; -.
DR   PhylomeDB; Q1LV15; -.
DR   TreeFam; TF323481; -.
DR   PRO; PR:Q1LV15; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005929; C:cilium; IEA:UniProtKB-SubCell.
DR   GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; IBA:GO_Central.
DR   GO; GO:0070286; P:axonemal dynein complex assembly; IMP:UniProtKB.
DR   GO; GO:0060271; P:cilium assembly; IMP:UniProtKB.
DR   GO; GO:0060285; P:cilium-dependent cell motility; IMP:UniProtKB.
DR   GO; GO:0007368; P:determination of left/right symmetry; IMP:UniProtKB.
DR   GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 4.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF00400; WD40; 8.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 8.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 3.
DR   PROSITE; PS50082; WD_REPEATS_2; 8.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..415
FT                   /note="Dynein assembly factor with WDR repeat domains 1"
FT                   /id="PRO_0000242657"
FT   REPEAT          90..129
FT                   /note="WD 1"
FT   REPEAT          132..172
FT                   /note="WD 2"
FT   REPEAT          175..214
FT                   /note="WD 3"
FT   REPEAT          217..256
FT                   /note="WD 4"
FT   REPEAT          259..298
FT                   /note="WD 5"
FT   REPEAT          301..340
FT                   /note="WD 6"
FT   REPEAT          343..384
FT                   /note="WD 7"
FT   REPEAT          385..415
FT                   /note="WD 8"
FT   CONFLICT        261
FT                   /note="R -> H (in Ref. 2; AAI24404)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   415 AA;  45934 MW;  A903A3F90FD35C72 CRC64;
     MRLKRMHIRY YPPGIFLDYE KGGQLRTKCI DLLNLTPETD VEELVLEIRA AEPLITSSCV
     EQVKLLICKL QEKVGQKDER KFYLFRALQA HILPLTNVAF NKSGSCFITG SYDRTCKIWD
     TASGEELHTL EGHRNVVYAI AFNNPYGDKV ATGSFDKTCK LWSAETGKCF YTFRGHTAEI
     VCLAFNPQST LVATGSMDTT AKLWDVESGE EVSTLAGHFA EIISLCFNTT GDRLVTGSFD
     HTAILWDVPS GRKVHVLSGH RGEISCVQFN WDCSLIATAS LDKSCKVWDA EGGQCLATLL
     GHNDEVLDVC FNYTGQLIAT ASADGTSRVF STDTFQCLCQ LEGHKGEISK VCFNAQGSRV
     LTASVDKTSR VWCVKTGACL QVLEGHSDEI FSCAFNYEGD TIITGSKDNT CRIWH