ID   DB10_NICSY              Reviewed;         607 AA.
AC   P46942;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=ATP-dependent RNA helicase-like protein DB10;
DE            EC=3.6.4.13;
OS   Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4096;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8111024; DOI=10.1007/bf00040593;
RA   Itadani H., Sugita M., Sugiura M.;
RT   "Structure and expression of a cDNA encoding an RNA helicase-like protein
RT   in tobacco.";
RL   Plant Mol. Biol. 24:249-252(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
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DR   EMBL; D16247; BAA03763.1; -; mRNA.
DR   PIR; S42639; S42639.
DR   RefSeq; NP_001289543.1; NM_001302614.1.
DR   AlphaFoldDB; P46942; -.
DR   SMR; P46942; -.
DR   STRING; 4096.XP_009768647.1; -.
DR   GeneID; 104219642; -.
DR   eggNOG; KOG0331; Eukaryota.
DR   Proteomes; UP000189701; Unplaced.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   CDD; cd00201; WW; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   InterPro; IPR001202; WW_dom.
DR   InterPro; IPR036020; WW_dom_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00456; WW; 1.
DR   SUPFAM; SSF51045; SSF51045; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
DR   PROSITE; PS01159; WW_DOMAIN_1; 1.
DR   PROSITE; PS50020; WW_DOMAIN_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..607
FT                   /note="ATP-dependent RNA helicase-like protein DB10"
FT                   /id="PRO_0000055118"
FT   DOMAIN          18..52
FT                   /note="WW"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00224"
FT   DOMAIN          176..350
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          379..523
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          66..108
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          519..607
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           145..173
FT                   /note="Q motif"
FT   MOTIF           298..301
FT                   /note="DEAD box"
FT   COMPBIAS        562..588
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        589..607
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         189..196
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   607 AA;  65989 MW;  F3E7A6E81B6645F3 CRC64;
     MAVVTASSAG PSYAPEDPTL PKPWKGLVDG TTGFIYFWNP ETNDTQYERP VPSSHAVSAP
     AHKSSVFVSS SVEKPSQGQR YDADGGHNRG SNNKIARSSS DRFHDGTSVH EGYGSLGVGS
     DISQESYCRR NEISVTGGDV PAPLTSFEAT GFPSEIVREM HQAGFSAPTP IQAQSWPIAL
     QGRDIVAIAK TGSGKTLGYL MPAFIHLQQR RKNPQLGPTI LVLSPTRELA TQIQAEAVKF
     GKSSRISCTC LYGGAPKGPQ LRELSRGVDI VVATPGRLND ILEMRRVSLG QVSYLVLDEA
     DRMLDMGFEP QIRKIVKEVP VQRQTLMYTA TWPKGVRKIA ADLLVNSVQV NIGNVDELVA
     NKSITQHIEV VLPMEKQRRV EQILRSKEPG SKIIIFCSTK KMCDQLSRNL TRNFGAAAIH
     GDKSQGERDY VLSQFRAGRS PVLVATDVAA RGLDIKDIRV VINYDFPTGI EDYVHRIGRT
     GRAGASGLAY TFFSDQDSKH ALDLVKVLEG ANQCVPTELR DMASRGGGMG RARNHWGSGP
     GGRGGRGGPY NSSYVGRNGG HGYDRGSRDS DRYGHGTYNA DAPRKRSRSR SPNIGSGWSG
     KKSRFTD