DB114_HUMAN
ID DB114_HUMAN Reviewed; 69 AA.
AC Q30KQ6; Q8NES9;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Beta-defensin 114;
DE AltName: Full=Beta-defensin 14;
DE Short=DEFB-14;
DE AltName: Full=Defensin, beta 114;
DE Flags: Precursor;
GN Name=DEFB114; Synonyms=DEFB14;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=16033865; DOI=10.1152/physiolgenomics.00104.2005;
RA Patil A.A., Cai Y., Sang Y., Blecha F., Zhang G.;
RT "Cross-species analysis of the mammalian beta-defensin gene family:
RT presence of syntenic gene clusters and preferential expression in the male
RT reproductive tract.";
RL Physiol. Genomics 23:5-17(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 31-69.
RX PubMed=11854508; DOI=10.1073/pnas.042692699;
RA Schutte B.C., Mitros J.P., Bartlett J.A., Walters J.D., Jia H.P.,
RA Welsh M.J., Casavant T.L., McCray P.B. Jr.;
RT "Discovery of five conserved beta-defensin gene clusters using a
RT computational search strategy.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:2129-2133(2002).
RN [3]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=23482568; DOI=10.1074/jbc.m112.411884;
RA Yu H., Dong J., Gu Y., Liu H., Xin A., Shi H., Sun F., Zhang Y., Lin D.,
RA Diao H.;
RT "The novel human beta-defensin 114 regulates lipopolysaccharide (LPS)-
RT mediated inflammation and protects sperm from motility loss.";
RL J. Biol. Chem. 288:12270-12282(2013).
CC -!- FUNCTION: Has a salt-sensitive antimicrobial activity against Gram-
CC negative bacteria, including E.coli, Gram-positive, including S.aureus,
CC and fungi, including C.albicans. Binds to and neutralizes bacterial
CC lipopolysaccharides (LPS), abolishing TNF production by macrophages
CC challenged with LPS. Rescues the LPS-induced reduction of sperm
CC motility in vitro and may protect from LPS-induced lethality.
CC {ECO:0000269|PubMed:23482568}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in epididymis, predominantly in the caput
CC (at protein level). {ECO:0000269|PubMed:23482568}.
CC -!- SIMILARITY: Belongs to the beta-defensin family. {ECO:0000305}.
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DR EMBL; DQ012018; AAY59754.1; -; mRNA.
DR EMBL; AY122470; AAM93912.1; -; mRNA.
DR CCDS; CCDS34474.1; -.
DR RefSeq; NP_001032588.1; NM_001037499.1.
DR AlphaFoldDB; Q30KQ6; -.
DR SMR; Q30KQ6; -.
DR BioGRID; 128842; 9.
DR STRING; 9606.ENSP00000312702; -.
DR BioMuta; DEFB114; -.
DR DMDM; 85540943; -.
DR PaxDb; Q30KQ6; -.
DR PRIDE; Q30KQ6; -.
DR Antibodypedia; 64299; 8 antibodies from 4 providers.
DR DNASU; 245928; -.
DR Ensembl; ENST00000322066.4; ENSP00000312702.3; ENSG00000177684.4.
DR GeneID; 245928; -.
DR KEGG; hsa:245928; -.
DR MANE-Select; ENST00000322066.4; ENSP00000312702.3; NM_001037499.2; NP_001032588.1.
DR UCSC; uc011dwp.3; human.
DR CTD; 245928; -.
DR GeneCards; DEFB114; -.
DR HGNC; HGNC:18095; DEFB114.
DR HPA; ENSG00000177684; Tissue enriched (epididymis).
DR MIM; 615243; gene.
DR neXtProt; NX_Q30KQ6; -.
DR OpenTargets; ENSG00000177684; -.
DR PharmGKB; PA38491; -.
DR VEuPathDB; HostDB:ENSG00000177684; -.
DR eggNOG; ENOG502RVKZ; Eukaryota.
DR GeneTree; ENSGT00390000016155; -.
DR HOGENOM; CLU_205440_0_0_1; -.
DR InParanoid; Q30KQ6; -.
DR OMA; LHFLCYV; -.
DR OrthoDB; 1609004at2759; -.
DR PhylomeDB; Q30KQ6; -.
DR TreeFam; TF341328; -.
DR PathwayCommons; Q30KQ6; -.
DR Reactome; R-HSA-1461957; Beta defensins.
DR Reactome; R-HSA-1461973; Defensins.
DR BioGRID-ORCS; 245928; 59 hits in 1011 CRISPR screens.
DR GenomeRNAi; 245928; -.
DR Pharos; Q30KQ6; Tbio.
DR PRO; PR:Q30KQ6; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q30KQ6; protein.
DR Bgee; ENSG00000177684; Expressed in blood and 10 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0001530; F:lipopolysaccharide binding; IDA:UniProtKB.
DR GO; GO:0061760; P:antifungal innate immune response; IMP:UniProtKB.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:UniProtKB.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IMP:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IMP:UniProtKB.
DR GO; GO:0031665; P:negative regulation of lipopolysaccharide-mediated signaling pathway; IDA:UniProtKB.
DR GO; GO:0043407; P:negative regulation of MAP kinase activity; IMP:UniProtKB.
DR GO; GO:0032720; P:negative regulation of tumor necrosis factor production; IMP:UniProtKB.
DR GO; GO:0050729; P:positive regulation of inflammatory response; IMP:UniProtKB.
DR InterPro; IPR025933; Beta_defensin.
DR Pfam; PF13841; Defensin_beta_2; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Defensin; Disulfide bond; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..69
FT /note="Beta-defensin 114"
FT /id="PRO_0000045342"
FT DISULFID 29..57
FT /evidence="ECO:0000250"
FT DISULFID 36..50
FT /evidence="ECO:0000250"
FT DISULFID 40..58
FT /evidence="ECO:0000250"
FT CONFLICT 54
FT /note="R -> G (in Ref. 2; AAM93912)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 69 AA; 8318 MW; 3ACF561F6636F772 CRC64;
MRIFYYLHFL CYVTFILPAT CTLVNADRCT KRYGRCKRDC LESEKQIDIC SLPRKICCTE
KLYEEDDMF
