ID   DBAT_TAXCU              Reviewed;         440 AA.
AC   Q9M6E2;
DT   22-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=10-deacetylbaccatin III 10-O-acetyltransferase;
DE            Short=DBAT;
DE            EC=2.3.1.167;
OS   Taxus cuspidata (Japanese yew).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers II; Cupressales; Taxaceae;
OC   Taxus.
OX   NCBI_TaxID=99806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
RX   PubMed=10639122; DOI=10.1073/pnas.97.2.583;
RA   Walker K., Croteau R.B.;
RT   "Molecular cloning of a 10-deacetylbaccatin III-10-O-acetyl transferase
RT   cDNA from Taxus and functional expression in Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:583-587(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=10-deacetylbaccatin III + acetyl-CoA = baccatin III + CoA;
CC         Xref=Rhea:RHEA:20137, ChEBI:CHEBI:18193, ChEBI:CHEBI:32898,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.167;
CC   -!- PATHWAY: Alkaloid biosynthesis; taxol biosynthesis; baccatin III from
CC       10-deacetyl-2-debenzoylbaccatin III: step 2/2.
CC   -!- SUBUNIT: Monomer.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; AF193765; AAF27621.1; -; mRNA.
DR   PIR; T52320; T52320.
DR   AlphaFoldDB; Q9M6E2; -.
DR   SMR; Q9M6E2; -.
DR   KEGG; ag:AAF27621; -.
DR   BioCyc; MetaCyc:MON-13414; -.
DR   BRENDA; 2.3.1.167; 6225.
DR   UniPathway; UPA00842; UER00811.
DR   GO; GO:0050643; F:10-deacetylbaccatin III 10-O-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042617; P:paclitaxel biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   1: Evidence at protein level;
KW   Acyltransferase; Taxol biosynthesis; Transferase.
FT   CHAIN           1..440
FT                   /note="10-deacetylbaccatin III 10-O-acetyltransferase"
FT                   /id="PRO_0000147359"
FT   ACT_SITE        162
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        372
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   440 AA;  49053 MW;  85CABE5010A68AC3 CRC64;
     MAGSTEFVVR SLERVMVAPS QPSPKAFLQL STLDNLPGVR ENIFNTLLVY NASDRVSVDP
     AKVIRQALSK VLVYYSPFAG RLRKKENGDL EVECTGEGAL FVEAMADTDL SVLGDLDDYS
     PSLEQLLFCL PPDTDIEDIH PLVVQVTRFT CGGFVVGVSF CHGICDGLGA GQFLIAMGEM
     ARGEIKPSSE PIWKRELLKP EDPLYRFQYY HFQLICPPST FGKIVQGSLV ITSETINCIK
     QCLREESKEF CSAFEVVSAL AWIARTRALQ IPHSENVKLI FAMDMRKLFN PPLSKGYYGN
     FVGTVCAMDN VKDLLSGSLL RVVRIIKKAK VSLNEHFTST IVTPRSGSDE SINYENIVGF
     GDRRRLGFDE VDFGWGHADN VSLVQHGLKD VSVVQSYFLF IRPPKNNPDG IKILSFMPPS
     IVKSFKFEME TMTNKYVTKP