ID   DBB2_DOLAU              Reviewed;          33 AA.
AC   P83376;
DT   01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=Dolabellanin-B2;
OS   Dolabella auricularia (Shoulderblade sea cat).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Heterobranchia; Euthyneura; Tectipleura; Aplysiida; Aplysioidea;
OC   Aplysiidae; Dolabella.
OX   NCBI_TaxID=6511 {ECO:0000305};
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Body wall;
RX   PubMed=12590964; DOI=10.1016/s0145-305x(02)00105-2;
RA   Iijima R., Kisugi J., Yamazaki M.;
RT   "A novel antimicrobial peptide from the sea hare Dolabella auricularia.";
RL   Dev. Comp. Immunol. 27:305-311(2003).
CC   -!- FUNCTION: Has antibacterial activity against Gram-negative bacteria
CC       E.coli JM109 and DH5-alpha, H.influenza IID 983, and V.vulnificus RIMD
CC       2219009. Has antibacterial activity against Gram-positive bacteria
CC       S.aureus IID 1677, B.subtilis RIMD 0225014 and L.monocytogenes VIU206.
CC       Possesses antifungal activity against S.cerevisiae A581A, S.pombe IFO
CC       1628, C.albicans ATCC 36232 and TIMM-1623, and C.tropicalis TIMM-0313.
CC       {ECO:0000269|PubMed:12590964}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Contains two disulfide bonds.
CC   -!- PTM: Up to two of the methionines may be oxidized to methionine
CC       sulfoxides.
CC   -!- MASS SPECTROMETRY: Mass=3865.4; Method=MALDI; Note=Without methionine
CC       sulfoxide.; Evidence={ECO:0000269|PubMed:12590964};
CC   -!- MASS SPECTROMETRY: Mass=3883.5; Method=MALDI; Note=With 1 methionine
CC       sulfoxide.; Evidence={ECO:0000269|PubMed:12590964};
CC   -!- MASS SPECTROMETRY: Mass=3899.6; Method=MALDI; Note=With 2 methionine
CC       sulfoxides.; Evidence={ECO:0000269|PubMed:12590964};
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DR   AlphaFoldDB; P83376; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Fungicide; Secreted.
FT   PEPTIDE         1..33
FT                   /note="Dolabellanin-B2"
FT                   /id="PRO_0000044772"
SQ   SEQUENCE   33 AA;  3872 MW;  D55A3622C937927E CRC64;
     SHQDCYEALH KCMASHSKPF SCSMKFHMCL QQQ