ID   DBBT_TAXCU              Reviewed;         440 AA.
AC   Q9FPW3;
DT   22-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=2-alpha-hydroxytaxane 2-O-benzoyltransferase;
DE            Short=TBT;
DE            EC=2.3.1.166;
DE   AltName: Full=2-debenzoyl-7,13-diacetylbaccatin III-2-O-benzoyl transferase;
DE            Short=DBBT;
OS   Taxus cuspidata (Japanese yew).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers II; Cupressales; Taxaceae;
OC   Taxus.
OX   NCBI_TaxID=99806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
RX   PubMed=11095755; DOI=10.1073/pnas.250491997;
RA   Walker K., Croteau R.B.;
RT   "Taxol biosynthesis: molecular cloning of a benzoyl-CoA:taxane 2alpha-O-
RT   benzoyltransferase cDNA from Taxus and functional expression in Escherichia
RT   coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:13591-13596(2000).
CC   -!- FUNCTION: Catalyzes the conversion of 2-debenzoyl-7,13-diacetylbaccatin
CC       III, a semisynthetic substrate, to 7,13-diacetylbaccatin III.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=10-deacetyl-2-debenzoylbaccatin III + benzoyl-CoA = 10-
CC         deacetylbaccatin III + CoA; Xref=Rhea:RHEA:18741, ChEBI:CHEBI:18193,
CC         ChEBI:CHEBI:32897, ChEBI:CHEBI:57287, ChEBI:CHEBI:57369;
CC         EC=2.3.1.166;
CC   -!- PATHWAY: Alkaloid biosynthesis; taxol biosynthesis; baccatin III from
CC       10-deacetyl-2-debenzoylbaccatin III: step 1/2.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; AF297618; AAG38049.1; -; mRNA.
DR   AlphaFoldDB; Q9FPW3; -.
DR   SMR; Q9FPW3; -.
DR   KEGG; ag:AAG38049; -.
DR   BioCyc; MetaCyc:MON-13413; -.
DR   BRENDA; 2.3.1.166; 6225.
DR   UniPathway; UPA00842; UER00810.
DR   GO; GO:0050642; F:2-alpha-hydroxytaxane 2-O-benzoyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042617; P:paclitaxel biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   1: Evidence at protein level;
KW   Acyltransferase; Taxol biosynthesis; Transferase.
FT   CHAIN           1..440
FT                   /note="2-alpha-hydroxytaxane 2-O-benzoyltransferase"
FT                   /id="PRO_0000147360"
FT   ACT_SITE        158
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        367
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   440 AA;  50089 MW;  A1EC9DB403A6005A CRC64;
     MGRFNVDMIE RVIVAPCLQS PKNILHLSPI DNKTRGLTNI LSVYNASQRV SVSADPAKTI
     REALSKVLVY YPPFAGRLRN TENGDLEVEC TGEGAVFVEA MADNDLSVLQ DFNEYDPSFQ
     QLVFNLREDV NIEDLHLLTV QVTRFTCGGF VVGTRFHHSV SDGKGIGQLL KGMGEMARGE
     FKPSLEPIWN REMVKPEDIM YLQFDHFDFI HPPLNLEKSI QASMVISFER INYIKRCMME
     ECKEFFSAFE VVVALIWLAR TKSFRIPPNE YVKIIFPIDM RNSFDSPLPK GYYGNAIGNA
     CAMDNVKDLL NGSLLYALML IKKSKFALNE NFKSRILTKP STLDANMKHE NVVGCGDWRN
     LGFYEADFGW GNAVNVSPMQ QQREHELAMQ NYFLFLRSAK NMIDGIKILM FMPASMVKPF
     KIEMEVTINK YVAKICNSKL