ID   DBD34_SEDHY             Reviewed;          28 AA.
AC   P86833;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2011, sequence version 1.
DT   11-DEC-2019, entry version 12.
DE   RecName: Full=3,4-dihydroxybenzoate decarboxylase {ECO:0000303|PubMed:8655551};
DE            EC=4.1.1.63;
DE   Flags: Fragment;
OS   Sedimentibacter hydroxybenzoicus (Clostridium hydroxybenzoicum).
OC   Bacteria; Firmicutes; Tissierellia; Sedimentibacter.
OX   NCBI_TaxID=29345;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION,
RP   BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC   STRAIN=ATCC 51151 / DSM 7310 / JW/Z-1;
RX   PubMed=8655551; DOI=10.1128/jb.178.12.3539-3543.1996;
RA   He Z., Wiegel J.;
RT   "Purification and characterization of an oxygen-sensitive, reversible 3,4-
RT   dihydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum.";
RL   J. Bacteriol. 178:3539-3543(1996).
CC   -!- FUNCTION: Reversibly catalyzes the decarboxylation of 3,4-
CC       dihydroxybenzoate to catechol. Inactive toward 4-hydroxybenzoate and
CC       other benzoate derivatives. {ECO:0000269|PubMed:8655551}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3,4-dihydroxybenzoate + H(+) = catechol + CO2;
CC         Xref=Rhea:RHEA:22416, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:18135, ChEBI:CHEBI:36241; EC=4.1.1.63;
CC         Evidence={ECO:0000269|PubMed:8655551};
CC   -!- ACTIVITY REGULATION: Inhibited by oxygen. Completely inhibited by
CC       HgCl(2). Partially inhibited by ZnSO(4), 2,3,4-trihydroxybeonzoate and
CC       3,4,5-trihydroxybeonzoate. Unaffected by KCl, MnCl(2) or EDTA. Not
CC       stimulated by thiamine phosphate, pyridoxal 5'-phosphate or biotin. Not
CC       inhibited by hydroxylamine, NaBH(4) or avidin.
CC       {ECO:0000269|PubMed:8655551}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.6 mM for 3,4-dihydroxybenzoate {ECO:0000269|PubMed:8655551};
CC       pH dependence:
CC         Optimum pH is 7.0. Stable between pH 6.5 and 9.5.
CC         {ECO:0000269|PubMed:8655551};
CC       Temperature dependence:
CC         Optimum temperature is 50 degrees Celsius. Stable between 11 and 50
CC         degrees Celsius. At 55 degrees Celsius the half life is 1.5 hours.
CC         {ECO:0000269|PubMed:8655551};
CC   -!- SUBUNIT: Homopentamer. {ECO:0000269|PubMed:8655551}.
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DR   GO; GO:0050223; F:protocatechuate decarboxylase activity; IEA:UniProtKB-EC.
PE   1: Evidence at protein level;
KW   Decarboxylase; Direct protein sequencing; Lyase.
FT   CHAIN           1..>28
FT                   /note="3,4-dihydroxybenzoate decarboxylase"
FT                   /id="PRO_0000402826"
FT   NON_TER         28
FT                   /evidence="ECO:0000303|PubMed:8655551"
SQ   SEQUENCE   28 AA;  3124 MW;  82A5B766585812E8 CRC64;
     MNKVTDLRSA IELLKTIPGQ LIETNXDV