DBF4A_XENLA
ID DBF4A_XENLA Reviewed; 661 AA.
AC Q7ZZH7; Q6SCJ7;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Protein DBF4 homolog A;
GN Name=dbf4; Synonyms=dbf4a;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE,
RP PHOSPHORYLATION, AND INTERACTION WITH CDC7.
RC TISSUE=Ovary;
RX PubMed=14561731; DOI=10.1093/jb/mvg163;
RA Furukohri A., Sato N., Masai H., Arai K., Sugino A., Waga S.;
RT "Identification and characterization of a Xenopus homolog of Dbf4, a
RT regulatory subunit of the Cdc7 protein kinase required for the initiation
RT of DNA replication.";
RL J. Biochem. 134:447-457(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION
RP WITH CDC7.
RX PubMed=15222894; DOI=10.1186/1471-2199-5-5;
RA Jares P., Luciani M.G., Blow J.J.;
RT "A Xenopus Dbf4 homolog is required for Cdc7 chromatin binding and DNA
RT replication.";
RL BMC Mol. Biol. 5:5-5(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Oocyte;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP DEVELOPMENTAL STAGE, AND INTERACTION WITH CDC7.
RX PubMed=16204181; DOI=10.1101/gad.1339805;
RA Takahashi T.S., Walter J.C.;
RT "Cdc7-Drf1 is a developmentally regulated protein kinase required for the
RT initiation of vertebrate DNA replication.";
RL Genes Dev. 19:2295-2300(2005).
RN [5]
RP FUNCTION, PHOSPHORYLATION, DEVELOPMENTAL STAGE, AND INTERACTION WITH CDC7.
RX PubMed=16507577; DOI=10.1074/jbc.m510278200;
RA Silva T., Bradley R.H., Gao Y., Coue M.;
RT "Xenopus CDC7/DRF1 complex is required for the initiation of DNA
RT replication.";
RL J. Biol. Chem. 281:11569-11576(2006).
CC -!- FUNCTION: Regulatory subunit for cdc7 which activates its kinase
CC activity thereby playing a central role in DNA replication and cell
CC proliferation. Not required during the initiation of DNA replication in
CC egg and during early embryonic development but is required later and
CC throughout development. The complex cdc7-dbf4a phosphorylates mcm2
CC subunit. {ECO:0000269|PubMed:15222894, ECO:0000269|PubMed:16507577}.
CC -!- SUBUNIT: Forms a complex with cdc7.
CC -!- INTERACTION:
CC Q7ZZH7; Q98TA5: chaf1a-a; NbExp=2; IntAct=EBI-8563988, EBI-8563970;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14561731,
CC ECO:0000269|PubMed:15222894}.
CC -!- DEVELOPMENTAL STAGE: Present throughout development. During the cell
CC cycle progression, levels in egg extracts do not change. Appears during
CC the first 12 hours of development, and they further increases
CC significantly between 12 and 24 hours. Not present in oocytes I-VI but
CC it is detected at maturation. The level of protein remains constant
CC from mature oocytes to stage 8.5 embryos but increases after mid-
CC blastula (at protein level). {ECO:0000269|PubMed:14561731,
CC ECO:0000269|PubMed:16204181, ECO:0000269|PubMed:16507577}.
CC -!- PTM: Phosphorylated. {ECO:0000269|PubMed:14561731,
CC ECO:0000269|PubMed:16507577}.
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DR EMBL; AB095983; BAC76421.1; -; mRNA.
DR EMBL; AY460183; AAR22406.1; -; mRNA.
DR EMBL; BC070760; AAH70760.1; -; mRNA.
DR RefSeq; NP_001082606.1; NM_001089137.1.
DR RefSeq; XP_018121835.1; XM_018266346.1.
DR AlphaFoldDB; Q7ZZH7; -.
DR BioGRID; 99929; 2.
DR IntAct; Q7ZZH7; 1.
DR MINT; Q7ZZH7; -.
DR PRIDE; Q7ZZH7; -.
DR GeneID; 398599; -.
DR KEGG; xla:398599; -.
DR CTD; 398599; -.
DR Xenbase; XB-GENE-972996; dbf4.L.
DR OMA; SKKEAKC; -.
DR OrthoDB; 615385at2759; -.
DR Proteomes; UP000186698; Chromosome 6L.
DR Bgee; 398599; Expressed in egg cell and 19 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR Gene3D; 6.10.250.3410; -; 1.
DR InterPro; IPR001357; BRCT_dom.
DR InterPro; IPR006572; Znf_DBF.
DR InterPro; IPR038545; Znf_DBF_sf.
DR Pfam; PF07535; zf-DBF; 1.
DR SMART; SM00586; ZnF_DBF; 1.
DR PROSITE; PS50172; BRCT; 1.
DR PROSITE; PS51265; ZF_DBF4; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Metal-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Repeat; Zinc; Zinc-finger.
FT CHAIN 1..661
FT /note="Protein DBF4 homolog A"
FT /id="PRO_0000317552"
FT DOMAIN 18..111
FT /note="BRCT 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00033"
FT DOMAIN 136..161
FT /note="BRCT 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00033"
FT ZN_FING 269..317
FT /note="DBF4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00600"
FT REGION 86..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 234..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 87..106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 244..264
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 276
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00600"
FT BINDING 279
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00600"
FT BINDING 289
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00600"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00600"
FT CONFLICT 5
FT /note="V -> I (in Ref. 2; AAR22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 184
FT /note="P -> L (in Ref. 2; AAR22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 390
FT /note="A -> V (in Ref. 2; AAR22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 431
FT /note="N -> H (in Ref. 2; AAR22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 446
FT /note="F -> S (in Ref. 2; AAR22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 448
FT /note="A -> T (in Ref. 2; AAR22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 464
FT /note="K -> E (in Ref. 2; AAR22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 535
FT /note="Missing (in Ref. 2; AAR22406)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 661 AA; 73699 MW; D09D85CBB8B9F274 CRC64;
MKSTVAAVKN PTGKVQADVC KPFTGKLFYL DLTSKLISEK LEKDIKELGG AIEGFLSKEI
SYLITSKKEA KCVKTLKYVC SIPSPEAAPN TGESSTSPGN RRLCQEGNSS KKNEKSLVSR
GKSLVKRAIK EQEILPKNSI LSNALNWGVK ILHVEEAKHY IEKKKTALQQ VRKSQPVVKV
ESKPQTRRKV KPQKLKSPYI KVEDCSCQYR PLYLVLPQFR SFQNPVSNYS VEVDKKADPG
QKLPETKQSV NKTGHGQDGA NNPNIKLKEQ KKHGYCECCL KKYDDLESHI LSPQHKNFSE
SAYYQVVDDL ISTFDFDFVD WSKYKNGRKG VGILMLAEKS KTEGQERNEA NLFVSKTHNF
SERVTATTPL QENTLKDQHA SPCSLPCTPA CNADQMFSLP SPAGSARLCN KKYKMDSNFG
QLVATPLPSF NLKDSLTGSF GGREMFVAKL NETMEPATEG PKIKWDVCNI PSNISQCIPQ
KVDPIAQHVN QFPNGNTHCS EMTACQAALI YENVDSAADA NIKLPKTVND LHNKEGHRAT
DKIYPTVQSH EYLPDYSPSG NLHRKVKTSA HRNKNKDELF CRLSHKVSVP QQEDDSKFPS
ETLLALFESS EDKTEFFGFA GSPAYESCSM DDGDTPDQTH KKMLLSLFPH TTTSGSSFLG
F
