ID   DBHA_ECOLW              Reviewed;          90 AA.
AC   E0J6W8; H9Y8Y2;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=DNA-binding protein HU-alpha;
DE   AltName: Full=HU-2;
DE   AltName: Full=NS2;
GN   Name=hupA; OrderedLocusNames=ECW_m4359, WFL_21215;
GN   ORFNames=EschWDRAFT_4326;
OS   Escherichia coli (strain ATCC 9637 / CCM 2024 / DSM 1116 / LMG 11080 / NBRC
OS   13500 / NCIMB 8666 / NRRL B-766 / W).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=566546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9637 / CCM 2024 / DSM 1116 / LMG 11080 / NBRC 13500 / NCIMB
RC   8666 / NRRL B-766 / W;
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Land M.L., Hauser L., Chang Y.-J., Jeffries C., Tremaine M.,
RA   Landick R., Keating D., Woyke T.J.;
RT   "The draft genome of Escherichia coli W.";
RL   Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9637 / CCM 2024 / DSM 1116 / LMG 11080 / NBRC 13500 / NCIMB
RC   8666 / NRRL B-766 / W;
RX   PubMed=21208457; DOI=10.1186/1471-2164-12-9;
RA   Archer C.T., Kim J.F., Jeong H., Park J.H., Vickers C.E., Lee S.Y.,
RA   Nielsen L.K.;
RT   "The genome sequence of E. coli W (ATCC 9637): comparative genome analysis
RT   and an improved genome-scale reconstruction of E. coli.";
RL   BMC Genomics 12:9-9(2011).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9637 / CCM 2024 / DSM 1116 / LMG 11080 / NBRC 13500 / NCIMB
RC   8666 / NRRL B-766 / W;
RX   PubMed=22075923; DOI=10.1007/s10295-011-1052-2;
RA   Turner P.C., Yomano L.P., Jarboe L.R., York S.W., Baggett C.L.,
RA   Moritz B.E., Zentz E.B., Shanmugam K.T., Ingram L.O.;
RT   "Optical mapping and sequencing of the Escherichia coli KO11 genome reveal
RT   extensive chromosomal rearrangements, and multiple tandem copies of the
RT   Zymomonas mobilis pdc and adhB genes.";
RL   J. Ind. Microbiol. Biotechnol. 39:629-639(2012).
RN   [4]
RP   MASS SPECTROMETRY.
RC   STRAIN=ATCC 9637 / CCM 2024 / DSM 1116 / LMG 11080 / NBRC 13500 / NCIMB
RC   8666 / NRRL B-766 / W;
RX   PubMed=9921691;
RX   DOI=10.1002/(sici)1097-0231(19990115)13:1<73::aid-rcm454>3.0.co;2-n;
RA   Dai Y., Li L., Roser D.C., Long S.R.;
RT   "Detection and identification of low-mass peptides and proteins from
RT   solvent suspensions of Escherichia coli by high performance liquid
RT   chromatography fractionation and matrix-assisted laser
RT   desorption/ionization mass spectrometry.";
RL   Rapid Commun. Mass Spectrom. 13:73-78(1999).
CC   -!- FUNCTION: Histone-like DNA-binding protein which is capable of wrapping
CC       DNA to stabilize it, and thus to prevent its denaturation under extreme
CC       environmental conditions. {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=9536.7; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:9921691};
CC   -!- SIMILARITY: Belongs to the bacterial histone-like protein family.
CC       {ECO:0000305}.
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DR   EMBL; AEDF01000040; EFN36065.1; -; Genomic_DNA.
DR   EMBL; CP002185; ADT77652.1; -; Genomic_DNA.
DR   EMBL; CP002967; AFH13884.1; -; Genomic_DNA.
DR   RefSeq; WP_001044513.1; NZ_WBMH01000055.1.
DR   AlphaFoldDB; E0J6W8; -.
DR   SMR; E0J6W8; -.
DR   PRIDE; E0J6W8; -.
DR   EnsemblBacteria; ADT77652; ADT77652; ECW_m4359.
DR   GeneID; 67415298; -.
DR   KEGG; ell:WFL_21215; -.
DR   KEGG; elw:ECW_m4359; -.
DR   PATRIC; fig|566546.30.peg.4418; -.
DR   HOGENOM; CLU_105066_3_1_6; -.
DR   OMA; ISQEKQC; -.
DR   Proteomes; UP000008525; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   Gene3D; 4.10.520.10; -; 1.
DR   InterPro; IPR000119; Hist_DNA-bd.
DR   InterPro; IPR020816; Histone-like_DNA-bd_CS.
DR   InterPro; IPR010992; IHF-like_DNA-bd_dom_sf.
DR   PANTHER; PTHR33175; PTHR33175; 1.
DR   Pfam; PF00216; Bac_DNA_binding; 1.
DR   PRINTS; PR01727; DNABINDINGHU.
DR   SMART; SM00411; BHL; 1.
DR   SUPFAM; SSF47729; SSF47729; 1.
DR   PROSITE; PS00045; HISTONE_LIKE; 1.
PE   1: Evidence at protein level;
KW   DNA condensation; DNA-binding.
FT   CHAIN           1..90
FT                   /note="DNA-binding protein HU-alpha"
FT                   /id="PRO_0000408737"
SQ   SEQUENCE   90 AA;  9535 MW;  6B5B8536FDBD13DC CRC64;
     MNKTQLIDVI AEKAELSKTQ AKAALESTLA AITESLKEGD AVQLVGFGTF KVNHRAERTG
     RNPQTGKEIK IAAANVPAFV SGKALKDAVK