ID   DBH_GEOSE               Reviewed;          90 AA.
AC   P0A3H0; P02346; P08822;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=DNA-binding protein HU;
DE   AltName: Full=DNA-binding protein II;
DE   AltName: Full=HB;
GN   Name=hup; Synonyms=hbs, hbsU;
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1644313; DOI=10.1016/0378-1119(92)90487-a;
RA   Padas P.M., Wilson K.S., Vorgias C.E.;
RT   "The DNA-binding protein HU from mesophilic and thermophilic bacilli: gene
RT   cloning, overproduction and purification.";
RL   Gene 117:39-44(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1503;
RX   PubMed=7765961; DOI=10.1271/bbb.59.126;
RA   Kawamura S., Kajiyama H., Yamasaki N., Kimura M.;
RT   "Cloning of the gene encoding DNA binding protein HU from Bacillus
RT   stearothermophilus and its expression in Escherichia coli.";
RL   Biosci. Biotechnol. Biochem. 59:126-129(1995).
RN   [3]
RP   PROTEIN SEQUENCE.
RX   PubMed=6300069; DOI=10.1016/s0021-9258(18)32768-6;
RA   Kimura M., Wilson K.S.;
RT   "On the DNA binding protein II from Bacillus stearothermophilus. II. The
RT   amino acid sequence and its relation to those of homologous proteins from
RT   other prokaryotes.";
RL   J. Biol. Chem. 258:4007-4011(1983).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (3 ANGSTROMS).
RX   PubMed=6540370; DOI=10.1038/310376a0;
RA   Tanaka I., Appelt K., Dijk J., White S.W., Wilson K.S.;
RT   "3-A resolution structure of a protein with histone-like properties in
RT   prokaryotes.";
RL   Nature 310:376-381(1984).
RN   [5]
RP   STRUCTURE BY NMR.
RX   PubMed=7500343; DOI=10.1006/jmbi.1995.0648;
RA   Vis H., Mariani M., Vorgias C.E., Wilson K.S., Kaptein R., Boelens R.;
RT   "Solution structure of the HU protein from Bacillus stearothermophilus.";
RL   J. Mol. Biol. 254:692-703(1995).
CC   -!- FUNCTION: Histone-like DNA-binding protein which is capable of wrapping
CC       DNA to stabilize it, and thus to prevent its denaturation under extreme
CC       environmental conditions.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the bacterial histone-like protein family.
CC       {ECO:0000305}.
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DR   EMBL; M73500; AAA22532.1; -; Genomic_DNA.
DR   EMBL; D38080; BAA07273.1; -; Genomic_DNA.
DR   PIR; JC1205; DNBS2F.
DR   PDB; 1HUE; NMR; -; A/B=1-90.
DR   PDB; 1HUU; X-ray; 2.00 A; A/B/C=1-90.
DR   PDBsum; 1HUE; -.
DR   PDBsum; 1HUU; -.
DR   AlphaFoldDB; P0A3H0; -.
DR   BMRB; P0A3H0; -.
DR   SMR; P0A3H0; -.
DR   PRIDE; P0A3H0; -.
DR   EvolutionaryTrace; P0A3H0; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   Gene3D; 4.10.520.10; -; 1.
DR   InterPro; IPR000119; Hist_DNA-bd.
DR   InterPro; IPR020816; Histone-like_DNA-bd_CS.
DR   InterPro; IPR010992; IHF-like_DNA-bd_dom_sf.
DR   PANTHER; PTHR33175; PTHR33175; 1.
DR   Pfam; PF00216; Bac_DNA_binding; 1.
DR   PRINTS; PR01727; DNABINDINGHU.
DR   SMART; SM00411; BHL; 1.
DR   SUPFAM; SSF47729; SSF47729; 1.
DR   PROSITE; PS00045; HISTONE_LIKE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; DNA condensation; DNA-binding;
KW   Phosphoprotein.
FT   CHAIN           1..90
FT                   /note="DNA-binding protein HU"
FT                   /id="PRO_0000104912"
FT   REGION          56..90
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         4
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250"
FT   HELIX           3..14
FT                   /evidence="ECO:0007829|PDB:1HUU"
FT   HELIX           18..37
FT                   /evidence="ECO:0007829|PDB:1HUU"
FT   STRAND          42..44
FT                   /evidence="ECO:0007829|PDB:1HUU"
FT   TURN            45..47
FT                   /evidence="ECO:0007829|PDB:1HUU"
FT   STRAND          48..55
FT                   /evidence="ECO:0007829|PDB:1HUU"
FT   STRAND          59..61
FT                   /evidence="ECO:0007829|PDB:1HUE"
FT   STRAND          63..70
FT                   /evidence="ECO:0007829|PDB:1HUE"
FT   STRAND          74..81
FT                   /evidence="ECO:0007829|PDB:1HUU"
FT   HELIX           83..89
FT                   /evidence="ECO:0007829|PDB:1HUU"
SQ   SEQUENCE   90 AA;  9716 MW;  4F51530D032C071E CRC64;
     MNKTELINAV AETSGLSKKD ATKAVDAVFD SITEALRKGD KVQLIGFGNF EVRERAARKG
     RNPQTGEEME IPASKVPAFK PGKALKDAVK