DBH_NOSS1
ID DBH_NOSS1 Reviewed; 94 AA.
AC P05514; Q57356;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=DNA-binding protein HU;
GN Name=hup; Synonyms=hanA; OrderedLocusNames=asr3935;
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8655556; DOI=10.1128/jb.178.12.3572-3577.1996;
RA Khudyakov I., Wolk C.P.;
RT "Evidence that the hanA gene coding for HU protein is essential for
RT heterocyst differentiation in, and cyanophage A-4(L) sensitivity of,
RT Anabaena sp. strain PCC 7120.";
RL J. Bacteriol. 178:3572-3577(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE.
RX PubMed=7748931; DOI=10.1016/0300-9084(94)90034-5;
RA Nagaraja R., Haselkorn R.;
RT "Protein HU from the cyanobacterium Anabaena.";
RL Biochimie 76:1082-1089(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
RN [4]
RP PROTEIN SEQUENCE.
RA Greene J.R.;
RL Unpublished observations (MAY-1984).
CC -!- FUNCTION: Histone-like DNA-binding protein which is capable of wrapping
CC DNA to stabilize it, and thus to prevent its denaturation under extreme
CC environmental conditions. It is essential for heterocyst
CC differentiation.
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the bacterial histone-like protein family.
CC {ECO:0000305}.
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DR EMBL; U46038; AAB06445.1; -; Genomic_DNA.
DR EMBL; S78244; AAB34352.1; -; Genomic_DNA.
DR EMBL; BA000019; BAB75634.1; -; Genomic_DNA.
DR PIR; AH2297; AH2297.
DR RefSeq; WP_010998076.1; NZ_RSCN01000045.1.
DR PDB; 1P51; X-ray; 2.50 A; A/B/C/D=1-94.
DR PDB; 1P71; X-ray; 1.90 A; A/B=1-94.
DR PDB; 1P78; X-ray; 2.25 A; A/B=1-94.
DR PDBsum; 1P51; -.
DR PDBsum; 1P71; -.
DR PDBsum; 1P78; -.
DR AlphaFoldDB; P05514; -.
DR SMR; P05514; -.
DR STRING; 103690.17133069; -.
DR EnsemblBacteria; BAB75634; BAB75634; BAB75634.
DR KEGG; ana:asr3935; -.
DR eggNOG; COG0776; Bacteria.
DR OMA; PAHEGIN; -.
DR OrthoDB; 1847472at2; -.
DR EvolutionaryTrace; P05514; -.
DR Proteomes; UP000002483; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR GO; GO:0043158; P:heterocyst differentiation; IEA:UniProtKB-KW.
DR Gene3D; 4.10.520.10; -; 1.
DR InterPro; IPR000119; Hist_DNA-bd.
DR InterPro; IPR020816; Histone-like_DNA-bd_CS.
DR InterPro; IPR010992; IHF-like_DNA-bd_dom_sf.
DR PANTHER; PTHR33175; PTHR33175; 1.
DR Pfam; PF00216; Bac_DNA_binding; 1.
DR PRINTS; PR01727; DNABINDINGHU.
DR SMART; SM00411; BHL; 1.
DR SUPFAM; SSF47729; SSF47729; 1.
DR PROSITE; PS00045; HISTONE_LIKE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA condensation; DNA-binding;
KW Heterocyst; Reference proteome.
FT CHAIN 1..94
FT /note="DNA-binding protein HU"
FT /id="PRO_0000104906"
FT CONFLICT 38
FT /note="S -> R (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 3..14
FT /evidence="ECO:0007829|PDB:1P71"
FT HELIX 18..37
FT /evidence="ECO:0007829|PDB:1P71"
FT STRAND 42..44
FT /evidence="ECO:0007829|PDB:1P71"
FT TURN 45..47
FT /evidence="ECO:0007829|PDB:1P71"
FT STRAND 48..55
FT /evidence="ECO:0007829|PDB:1P71"
FT STRAND 58..61
FT /evidence="ECO:0007829|PDB:1P71"
FT TURN 63..65
FT /evidence="ECO:0007829|PDB:1P71"
FT STRAND 68..71
FT /evidence="ECO:0007829|PDB:1P71"
FT STRAND 74..81
FT /evidence="ECO:0007829|PDB:1P71"
FT HELIX 83..89
FT /evidence="ECO:0007829|PDB:1P71"
SQ SEQUENCE 94 AA; 10073 MW; 045BD1FAE4F85DDF CRC64;
MNKGELVDAV AEKASVTKKQ ADAVLTAALE TIIEAVSSGD KVTLVGFGSF ESRERKAREG
RNPKTNEKME IPATRVPAFS AGKLFREKVA PPKA
