ID   DBNBT_TAXCA             Reviewed;         441 AA.
AC   Q8LL69;
DT   22-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase {ECO:0000303|PubMed:12089320};
DE            Short=DBTNBT {ECO:0000303|PubMed:12089320};
DE            EC=2.3.1.- {ECO:0000269|PubMed:12089320};
GN   Name=TAX10 {ECO:0000303|PubMed:12089320};
OS   Taxus canadensis (Canadian yew).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers II; Cupressales; Taxaceae;
OC   Taxus.
OX   NCBI_TaxID=88032;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=12089320; DOI=10.1073/pnas.082115799;
RA   Walker K., Long R., Croteau R.B.;
RT   "The final acylation step in taxol biosynthesis: cloning of the taxoid C13-
RT   side-chain N-benzoyltransferase from Taxus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:9166-9171(2002).
CC   -!- FUNCTION: Catalyzes the stereoselective coupling of the surrogate
CC       substrate N-debenzoyl-(3'RS)-2'-deoxytaxol with benzoyl-CoA to form
CC       predominantly one 3'-epimer of 2'-deoxytaxol (PubMed:12089320). This
CC       enzymatic reaction constitutes the final acylation in the taxol
CC       biosynthetic pathway (PubMed:12089320). {ECO:0000269|PubMed:12089320}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3'-N-debenzoyltaxol + benzoyl-CoA = CoA + H(+) + taxol;
CC         Xref=Rhea:RHEA:33687, ChEBI:CHEBI:15378, ChEBI:CHEBI:45863,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57369, ChEBI:CHEBI:63863;
CC         Evidence={ECO:0000269|PubMed:12089320};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33688;
CC         Evidence={ECO:0000269|PubMed:12089320};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.45 mM for 3'-N-debenzoyltaxol {ECO:0000269|PubMed:12089320};
CC         KM=0.41 mM for benzoyl-CoA {ECO:0000269|PubMed:12089320};
CC       pH dependence:
CC         Optimum pH is 8.0. {ECO:0000269|PubMed:12089320};
CC   -!- PATHWAY: Alkaloid biosynthesis; taxol biosynthesis. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; AF466397; AAM75818.1; -; mRNA.
DR   AlphaFoldDB; Q8LL69; -.
DR   SMR; Q8LL69; -.
DR   PRIDE; Q8LL69; -.
DR   BioCyc; MetaCyc:MON-13418; -.
DR   UniPathway; UPA00842; -.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042617; P:paclitaxel biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   1: Evidence at protein level;
KW   Acyltransferase; Taxol biosynthesis; Transferase.
FT   CHAIN           1..441
FT                   /note="3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase"
FT                   /id="PRO_0000147361"
FT   ACT_SITE        163
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        373
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   441 AA;  49041 MW;  1F0C200FDA6D4A0C CRC64;
     MEKAGSTDFH VKKFDPVMVA PSLPSPKATV QLSVVDSLTI CRGIFNTLLV FNAPDNISAD
     PVKIIREALS KVLVYYFPLA GRLRSKEIGE LEVECTGDGA LFVEAMVEDT ISVLRDLDDL
     NPSFQQLVFW HPLDTAIEDL HLVIVQVTRF TCGGIAVGVT LPHSVCDGRG AAQFVTALAE
     MARGEVKPSL EPIWNRELLN PEDPLHLQLN QFDSICPPPM LEELGQASFV INVDTIEYMK
     QCVMEECNEF CSSFEVVAAL VWIARTKALQ IPHTENVKLL FAMDLRKLFN PPLPNGYYGN
     AIGTAYAMDN VQDLLNGSLL RAIMIIKKAK ADLKDNYSRS RVVTNPYSLD VNKKSDNILA
     LSDWRRLGFY EADFGWGGPL NVSSLQRLEN GLPMFSTFLY LLPAKNKSDG IKLLLSCMPP
     TTLKSFKIVM EAMIEKYVSK V