ID   DBP10_PICST             Reviewed;         931 AA.
AC   A3LZT3;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 2.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=ATP-dependent RNA helicase DBP10;
DE            EC=3.6.4.13;
GN   Name=DBP10; ORFNames=PICST_80003;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: ATP-binding RNA helicase involved in the biogenesis of 60S
CC       ribosomal subunits and is required for the normal formation of 25S and
CC       5.8S rRNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC       family of RNA helicases and controls ATP binding and hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX54/DBP10
CC       subfamily. {ECO:0000305}.
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DR   EMBL; CP000502; ABN68395.2; -; Genomic_DNA.
DR   RefSeq; XP_001386424.2; XM_001386387.1.
DR   AlphaFoldDB; A3LZT3; -.
DR   SMR; A3LZT3; -.
DR   STRING; 4924.XP_001386424.2; -.
DR   EnsemblFungi; ABN68395; ABN68395; PICST_80003.
DR   GeneID; 4841046; -.
DR   KEGG; pic:PICST_80003; -.
DR   eggNOG; KOG0337; Eukaryota.
DR   HOGENOM; CLU_003041_5_2_1; -.
DR   InParanoid; A3LZT3; -.
DR   OMA; MRWDKKS; -.
DR   OrthoDB; 268859at2759; -.
DR   Proteomes; UP000002258; Chromosome 8.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR012541; DBP10_C.
DR   InterPro; IPR033517; DDX54/DBP10.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   PANTHER; PTHR24031:SF292; PTHR24031:SF292; 1.
DR   Pfam; PF08147; DBP10CT; 1.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM01123; DBP10CT; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW   Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT   CHAIN           1..931
FT                   /note="ATP-dependent RNA helicase DBP10"
FT                   /id="PRO_0000285154"
FT   DOMAIN          130..302
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          372..532
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          1..78
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          836..910
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           99..127
FT                   /note="Q motif"
FT   MOTIF           250..253
FT                   /note="DEAD box"
FT   COMPBIAS        24..43
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        44..59
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        848..910
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         143..150
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   931 AA;  105714 MW;  8344E8A4B7098C81 CRC64;
     MSDNEEDYDI ARSLTVNLED SDSDSGSDFS DEEQEVQDII SSEDEAEEQP KKKQKTAKPA
     KEAFPSLELS GDEDEQDDDK DMASYFAANN PQAKKAKAGS FQSFGLSKLV LTNIAKKGYR
     QPTPIQRKTI PLIMANRDVV GMARTGSGKT AAFTLPVIEK LKGHSARVGI RAIILSPSRE
     LALQTYKQVK EFSKGSDLRA IVLTGGDSLE DQFSSMVSNP DIVIATPGRF LHLQVEMQLD
     LKTVEYIVFD EADHLFEQGF AEQLNELLAV LPPQRQSLLF SATLPRSLVD FAKAGLSNPV
     LVRLDADSKI SDQLQMAFFT TKKNEREANL LYVLQEVIKM PLGTAEQIKK LRLMDKRVND
     EAEEEELENE SGSKRKYKFK KERLPSANVL PSPHSTIVFV PTKHHVEYIT TLLRDAGYLV
     SYIYGTLDQH ARKNQLYQFR LGMTTVLVVT DVAARGIDIP VLANVVNYTL PGSSKIFIHR
     VGRTARAGNK GWAYSIVNEK ELPYLLDLEL FLGKKILLTA MQEQKCQLLK DKQGDNYVEP
     KVQYTDRLVL GSCPRLSLET FEELYENLLR NHYELSVIKE VAAKGEKLYY RTRKAASTES
     VKRAKEIMDT GTWDDQHLLF GPNLEKEKEK FLAKLANRHV KETVFEFNKK GNDRDEDSLV
     SFMHRRRKQL APIQRRASER RDLLQREREA GLTHGIEDEI LKAHGETGYS ASGINDVDEE
     ELQNAFEDAD QLSSKSNKKN YRDDRFFISH YAPASVIQDQ QLNITSSFAN EAASATFDLD
     NDDKIGNGKQ VMQWDRKKGK YINSQSTDKK FIIGESGAKI PATYRSGKFD EWKKKRNMQP
     AKVGSLETEG ESKQRFKHKR NAAPKLPDKY RDDYHKQKKK VEKAVESGRD VKGYHKPGQR
     SEIKSTEDIR KARLLKEKKM AKNARPSRKR K