DBP4_ASPTN
ID DBP4_ASPTN Reviewed; 804 AA.
AC Q0CMM5;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=ATP-dependent RNA helicase dbp4;
DE EC=3.6.4.13;
GN Name=dbp4; ORFNames=ATEG_05059;
OS Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=341663;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIH 2624 / FGSC A1156;
RA Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA Nierman W.C., Milne T., Madden K.;
RT "Annotation of the Aspergillus terreus NIH2624 genome.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: ATP-dependent RNA helicase required for ribosome biogenesis.
CC Involved in the release of U14 snoRNA in pre-ribosomal complexes.
CC Required for pre-rRNA cleavage at site A2 (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBUNIT: Interacts with the U3 and U14 snoRNAs. Associates with pre-
CC ribosomal complexes (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX10/DBP4
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAU34128.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EAU34128.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; CH476600; EAU34128.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_001214237.1; XM_001214237.1.
DR AlphaFoldDB; Q0CMM5; -.
DR SMR; Q0CMM5; -.
DR STRING; 341663.Q0CMM5; -.
DR GeneID; 4321060; -.
DR eggNOG; KOG0342; Eukaryota.
DR eggNOG; KOG0343; Eukaryota.
DR OrthoDB; 973872at2759; -.
DR Proteomes; UP000007963; Unassembled WGS sequence.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0032040; C:small-subunit processome; IEA:EnsemblFungi.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0034512; F:box C/D RNA binding; IEA:EnsemblFungi.
DR GO; GO:0042802; F:identical protein binding; IEA:EnsemblFungi.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0034511; F:U3 snoRNA binding; IEA:EnsemblFungi.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR025313; DUF4217.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF13959; DUF4217; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM01178; DUF4217; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT CHAIN 1..804
FT /note="ATP-dependent RNA helicase dbp4"
FT /id="PRO_0000310199"
FT DOMAIN 77..251
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 277..436
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 493..541
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 589..615
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 695..804
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 46..74
FT /note="Q motif"
FT MOTIF 199..202
FT /note="DEAD box"
FT COMPBIAS 493..512
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 520..541
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 743..792
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 90..97
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 804 AA; 91077 MW; E2A168DC14BD33FA CRC64;
MAPANAPRNG KYAKSSQRTL KRKRVQEDLS SLVQRVEDLD LKESFKAFTD LPLSEPTLSG
LSASHYKTLT DIQSRAVSHA LKGRDILGAA KTGSGKTLAF LIPVLENLYR KQWAEHDGLG
ALILSPTREL AIQIFEVLRK VGRYHHFSAG LVIGGKSLKE EQERLGKMNI LVCTPGRMLQ
HLDQTALFDT YNLQMLVLDE ADRIMDMGFQ KTVDAIIGHL PKERQTMLFS ATQTKKVSDL
ARLSLQDPEY VAVHEAAASA TPSTLQQHYV VTPLPQKLDI LWSFIRSNLK SKTIVFLSSG
KQVRFVYEAF RHLQPGIPLM HLHGRQKQGG RLDITTKYSQ AKHAVLFSTD VAARGLDFPA
VDWVIQLDCP EDADTYIHRV GRTARYERDG RAVLFLDPSE EQGMLKRLEQ KKVPVEKINV
KANKQQSIKN QLQNMCFKDP ELKYLGQKAF ISYVKSVYVQ KDKEIFKLKD LDLEEFASSL
GLPGAPRIKF IKGDDTKERK NAPRAVAHLS SDDDESDAED DEKKSKKKDA PQVRTKYDRM
FERRNQDVLA GHYTKLINDD GTLADPKATD EADEDNDFLS VKRRFDAGDK QLEVGGSSDE
SGSDSEAETG KKDVKVVNID GKEPLVIDSK RREKLLKSKK KLLKFKGKGT KLIYDDEGNA
HEIYEMEDEE QFRAKGDAKE QQARFLAAEA ERTRLADVED KELVKQKRRE KKEKRKARER
ELLAQEEQEE MLVQLPPPED DDQGRFSPSE DEAPRPSKKQ RVQFAEPAES EEERRPKKPK
KSTAEPKEIQ TLEDLESLAT GLLG
