DBP7_CANGA
ID DBP7_CANGA Reviewed; 715 AA.
AC Q8TFL3; Q6FLJ0;
DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=ATP-dependent RNA helicase DBP7;
DE EC=3.6.4.13;
GN Name=DBP7; OrderedLocusNames=CAGL0L03047g;
OS Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS Y-65) (Yeast) (Torulopsis glabrata).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC Nakaseomyces/Candida clade.
OX NCBI_TaxID=284593;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX PubMed=12125055; DOI=10.1002/yea.890;
RA Walsh D.W., Wolfe K.H., Butler G.;
RT "Genomic differences between Candida glabrata and Saccharomyces cerevisiae
RT around the MRPL28 and GCN3 loci.";
RL Yeast 19:991-994(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: ATP-binding RNA helicase involved in the biogenesis of 60S
CC ribosomal subunits and is required for the normal formation of 25S and
CC 5.8S rRNAs. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- MISCELLANEOUS: Present with 1460 molecules/cell in log phase SD medium.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX31/DBP7
CC subfamily. {ECO:0000305}.
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DR EMBL; AY083607; AAM08097.1; -; Genomic_DNA.
DR EMBL; CR380958; CAG61874.1; -; Genomic_DNA.
DR RefSeq; XP_448904.1; XM_448904.1.
DR AlphaFoldDB; Q8TFL3; -.
DR SMR; Q8TFL3; -.
DR STRING; 5478.XP_448904.1; -.
DR EnsemblFungi; CAG61874; CAG61874; CAGL0L03047g.
DR GeneID; 2890843; -.
DR KEGG; cgr:CAGL0L03047g; -.
DR CGD; CAL0135220; CAGL0L03047g.
DR VEuPathDB; FungiDB:CAGL0L03047g; -.
DR eggNOG; KOG0348; Eukaryota.
DR HOGENOM; CLU_003041_26_2_1; -.
DR InParanoid; Q8TFL3; -.
DR OMA; QMGFERW; -.
DR Proteomes; UP000002428; Chromosome L.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR025313; DUF4217.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF13959; DUF4217; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM01178; DUF4217; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT CHAIN 1..715
FT /note="ATP-dependent RNA helicase DBP7"
FT /id="PRO_0000232253"
FT DOMAIN 160..354
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 400..586
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 94..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 668..698
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 125..154
FT /note="Q motif"
FT MOTIF 289..292
FT /note="DEAD box"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..69
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 683..698
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 173..180
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 715 AA; 79988 MW; C43196A9DD7E8306 CRC64;
MSDDESMVLN FSTDTAGIQR ADKVTGGRWK DRRKLQQRLG GREPVKRRAE GGDKERGDKR
AKVDTGNAQE KKNIPKPVSK ADVNINSQIV SSLFTSNRAK QTSENKNKHN SDDKVVPSNA
PLTDDSFEGL GVGSLVVSHL ENKMRIQKST SIQKVVIPQI LQNADKTDFF IHAQTGSGKT
LAYLLPIFSA ILGMGDHIDR KSGCFALIIA PTRELASQIY HVTTMLANCC HYLVPCLLIG
GERKKSEKAR LRKGCNFIIG TPGRILDHFQ NTKVIKEQMQ SSLRYVVLDE GDKLMELGFE
ETINQIMEIV NSMDVITRKY PKLPNRIVHL LCSATKNNEV AKLSKRSLDN YKVISIGGKK
DTMMDNTSVP DQLLQKVVIA PPKLRLITLA GVLDGIQKKP LDAGSVAKRT IVFLSCADSV
DYHFEVFSGN DGLYKNLVGD SVRVLSKGNK ILPSIKDEEL PGIICYKLHG SLSQQMRTMT
LKHFATDSEQ TKGKHLILFC TDVASRGLDL PDVSTVIEFD PPFAVEDHLH RIGRTARAGR
SGEALLFLLP GEEEGYLDYI QKYHPKGWDL LDAEKDVLIK AFNDIDVARN DKEIKSTGKT
FEWDTNATTW HLNVERRILE NEQFKEQATK GYISHVRAYA THISAEKQYF NLKGVHLGHL
AKSFGLRDRP KAMGMNSSKD ANGNERSKPK KENAKNKMFR MARMAAKQSA DEFNY
