DBP7_SCLS1
ID DBP7_SCLS1 Reviewed; 877 AA.
AC A7F8V8;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=ATP-dependent RNA helicase dbp7;
DE EC=3.6.4.13;
GN Name=dbp7; ORFNames=SS1G_14039;
OS Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold)
OS (Whetzelinia sclerotiorum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Helotiales; Sclerotiniaceae; Sclerotinia.
OX NCBI_TaxID=665079;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 18683 / 1980 / Ss-1;
RX PubMed=21876677; DOI=10.1371/journal.pgen.1002230;
RA Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A.,
RA Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E.,
RA Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M.,
RA Quevillon E., Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P.,
RA Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I.,
RA Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P.,
RA Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C.,
RA Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C.,
RA Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E.,
RA Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N.,
RA Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E.,
RA Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O.,
RA Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M.;
RT "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia
RT sclerotiorum and Botrytis cinerea.";
RL PLoS Genet. 7:E1002230-E1002230(2011).
CC -!- FUNCTION: ATP-binding RNA helicase involved in the biogenesis of 60S
CC ribosomal subunits and is required for the normal formation of 25S and
CC 5.8S rRNAs. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX31/DBP7
CC subfamily. {ECO:0000305}.
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DR EMBL; CH476649; EDN99179.1; -; Genomic_DNA.
DR RefSeq; XP_001584942.1; XM_001584892.1.
DR AlphaFoldDB; A7F8V8; -.
DR SMR; A7F8V8; -.
DR STRING; 665079.A7F8V8; -.
DR EnsemblFungi; EDN99179; EDN99179; SS1G_14039.
DR GeneID; 5481078; -.
DR KEGG; ssl:SS1G_14039; -.
DR VEuPathDB; FungiDB:sscle_10g079110; -.
DR eggNOG; KOG0348; Eukaryota.
DR HOGENOM; CLU_003041_26_2_1; -.
DR InParanoid; A7F8V8; -.
DR OMA; AVHIKAD; -.
DR Proteomes; UP000001312; Unassembled WGS sequence.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR GO; GO:0042254; P:ribosome biogenesis; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR025313; DUF4217.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF13959; DUF4217; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM01178; DUF4217; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT CHAIN 1..877
FT /note="ATP-dependent RNA helicase dbp7"
FT /id="PRO_0000310218"
FT DOMAIN 288..484
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 512..719
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 34..163
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 225..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 803..851
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 255..284
FT /note="Q motif"
FT MOTIF 417..420
FT /note="DEAD box"
FT COMPBIAS 51..88
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 89..103
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 813..849
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 301..308
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 877 AA; 96640 MW; 1BDA4018C32FF3D3 CRC64;
MADDGMLMNF EIGEVPIVTK QSFKGGRWKD RLAAKKTAQH RVSRSTSKPS TREIFSERQH
DTGAEEYIGR EPSLRAPKRQ RVDDNYDSYG GRNESTAAYA SGKLPSGSIN LGRGRKTTFQ
EETRPAFVAG KLPPGSINIS GKKATPIQEE TRPAFVSGKL PPGSINSGAR KAISFQEEKK
PAYISGKLPH GSIDGMRNRE MAAVHREIAE GGRKPGQVVS SLFTFNPTSK KKFDEPEEES
EPAKPSNAPL TEEMATFTNL GLSRRLAAHL STKLDMKAPT AIQKASVTQL ISDDSDAFIQ
AETGSGKTLA YLLPIVERIL ALSDNGIQIH RDSGLFAIIL SPTRELCKQI AAVLEKVLRC
APWIVGTTVN GGESKQSEKA RLRKGVNILV ATPGRLADHL DNTEVLNVAT VRWLVLDEGD
RLMELGFEEE IKGIVEKIGR RSVASGSSEM MSLPKRRVTI LCSATMKMNV QRLGEISLKD
AVHIQADPSE QEKQDKANGI EADDKAFSAP TQLKQSYAIV PAKLRLVTLT ALLKRAFARK
GSVMKAIVFM SCADSVDFHF SLFSRSAEKS AEASEEGKVD PPTLPKSELI KETITHGATI
SNNSNPVILH KLHGSLAQNI RTATLKAYSE SADPCVLICT DVASRGLDLP NVDFVIEYDP
PFSAEDHLHR VGRTARAGRE GRALIFLMPG TEEEYVSILA SGYREGRKAL THHTAEDLIQ
KGFGGTGREW EERATNFQLE VERWSLDSPR YLEMARRGFQ SHIRAYATHV ANERHIFNMQ
ELHLGHLAKA FALRDKPGSI KVPGLRPAKM TKADRSVAAR KAKRGEKEEE KAPEGERVRK
QRKMELDLPT VDSNEVAARM KRKMKEHMSA ASEFNIG
