DBR1_ARATH
ID DBR1_ARATH Reviewed; 418 AA.
AC Q94K01; O81771;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Lariat debranching enzyme;
DE Short=AtDBR1;
DE EC=3.1.-.-;
GN Name=DBR1; OrderedLocusNames=At4g31770; ORFNames=F28M20.40;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=14570879; DOI=10.1074/jbc.m309106200;
RA Wang H., Hill K., Perry S.E.;
RT "An Arabidopsis RNA lariat debranching enzyme is essential for
RT embryogenesis.";
RL J. Biol. Chem. 279:1468-1473(2004).
CC -!- FUNCTION: Cleaves the 2'-5' phosphodiester linkage at the branch point
CC of lariat intron pre-mRNAs after splicing and converts them into linear
CC molecules that are subsequently degraded. It thereby facilitates
CC ribonucleotide turnover. It may also participate in retrovirus
CC replication via an RNA lariat intermediate in cDNA synthesis. Plays en
CC essential role during embryogenesis. {ECO:0000269|PubMed:14570879}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Widely expressed. Expressed in roots, stems,
CC cauline and rosette leaves, flower buds and siliques.
CC {ECO:0000269|PubMed:14570879}.
CC -!- SIMILARITY: Belongs to the lariat debranching enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA19746.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79895.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL031004; CAA19746.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161579; CAB79895.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85955.1; -; Genomic_DNA.
DR EMBL; AF370499; AAK43876.1; -; mRNA.
DR PIR; T05093; T05093.
DR RefSeq; NP_567881.1; NM_119326.4.
DR AlphaFoldDB; Q94K01; -.
DR SMR; Q94K01; -.
DR STRING; 3702.AT4G31770.1; -.
DR PaxDb; Q94K01; -.
DR PRIDE; Q94K01; -.
DR ProteomicsDB; 223982; -.
DR DNASU; 829305; -.
DR EnsemblPlants; AT4G31770.1; AT4G31770.1; AT4G31770.
DR GeneID; 829305; -.
DR Gramene; AT4G31770.1; AT4G31770.1; AT4G31770.
DR KEGG; ath:AT4G31770; -.
DR Araport; AT4G31770; -.
DR TAIR; locus:2124829; AT4G31770.
DR eggNOG; KOG2863; Eukaryota.
DR HOGENOM; CLU_005893_2_0_1; -.
DR InParanoid; Q94K01; -.
DR OrthoDB; 1047278at2759; -.
DR PhylomeDB; Q94K01; -.
DR PRO; PR:Q94K01; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q94K01; baseline and differential.
DR Genevisible; Q94K01; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0008419; F:RNA lariat debranching enzyme activity; IDA:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd00844; MPP_Dbr1_N; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR007708; DBR1_C.
DR InterPro; IPR041816; Dbr1_N.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR Pfam; PF05011; DBR1; 1.
DR Pfam; PF00149; Metallophos; 1.
DR SMART; SM01124; DBR1; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Hydrolase; mRNA processing; Nucleus;
KW Reference proteome.
FT CHAIN 1..418
FT /note="Lariat debranching enzyme"
FT /id="PRO_0000250369"
FT REGION 372..418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 391..407
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 418 AA; 48175 MW; BE8AD41372C9A390 CRC64;
MKIAIEGCMH GDLDNVYKTI QHYEQIHNTK VDLLLCCGDF QAVRNEKDMD SLNVPRKYRE
MKSFWKYYSG QEVAPIPTIF IGGNHEASNY LWELYYGGWA ATNIYFLGFA GVVKFGNVRI
GGLSGIYNER HYRSGHFERP PYNESTIRSV YHVRDYDVQK LMQLEEPLDI FLSHDWPVGI
TDYGDSESLM RQKPYFRQEI EEKTLGSKPA ALLLEKLKPQ YWFSAHLHCK FAAAVQHGND
GSVTKFLALD KCLPGKKFLQ IIEIESEPGP FEVLYDEEWL AITRKFNSIF PLTRRYTNVS
TAGTIQESRE WVRKKLEERQ FKPFEFARTV PAYNPSQRVF DSIPEIPQNP QTLSLLELLG
LPYLLDSSPV TGERTDIPAS LAPSDLPTYD SEEIPIDDID EIEEMEEAKA DDHTRDDA
