ID   DBR1_CAEEL              Reviewed;         500 AA.
AC   Q966M6; P90972;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 2.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Lariat debranching enzyme;
DE            EC=3.1.-.-;
DE   AltName: Full=Ce-dbr1;
GN   Name=dbr-1; ORFNames=C55B7.8;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9001235; DOI=10.1128/mcb.17.2.809;
RA   Nam K., Lee G., Trambley J., Devine S.E., Boeke J.D.;
RT   "Severe growth defect in a Schizosaccharomyces pombe mutant defective in
RT   intron lariat degradation.";
RL   Mol. Cell. Biol. 17:809-818(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Cleaves the 2'-5' phosphodiester linkage at the branch point
CC       of lariat intron pre-mRNAs after splicing and converts them into linear
CC       molecules that are subsequently degraded. It thereby facilitates
CC       ribonucleotide turnover. It may also participate in retrovirus
CC       replication via an RNA lariat intermediate in cDNA synthesis (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the lariat debranching enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; U63636; AAC47433.1; -; Genomic_DNA.
DR   EMBL; FO080958; CCD68095.1; -; Genomic_DNA.
DR   RefSeq; NP_491868.2; NM_059467.5.
DR   AlphaFoldDB; Q966M6; -.
DR   SMR; Q966M6; -.
DR   BioGRID; 37808; 12.
DR   STRING; 6239.C55B7.8; -.
DR   EPD; Q966M6; -.
DR   PaxDb; Q966M6; -.
DR   PeptideAtlas; Q966M6; -.
DR   PRIDE; Q966M6; -.
DR   EnsemblMetazoa; C55B7.8.1; C55B7.8.1; WBGene00000937.
DR   GeneID; 172357; -.
DR   KEGG; cel:CELE_C55B7.8; -.
DR   UCSC; C55B7.8; c. elegans.
DR   CTD; 172357; -.
DR   WormBase; C55B7.8; CE30437; WBGene00000937; dbr-1.
DR   eggNOG; KOG2863; Eukaryota.
DR   GeneTree; ENSGT00510000047481; -.
DR   HOGENOM; CLU_005893_0_0_1; -.
DR   InParanoid; Q966M6; -.
DR   OMA; GIDDPLC; -.
DR   OrthoDB; 1047278at2759; -.
DR   PhylomeDB; Q966M6; -.
DR   PRO; PR:Q966M6; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00000937; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0008419; F:RNA lariat debranching enzyme activity; ISS:UniProtKB.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   GO; GO:0000375; P:RNA splicing, via transesterification reactions; ISS:UniProtKB.
DR   CDD; cd00844; MPP_Dbr1_N; 1.
DR   InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR   InterPro; IPR007708; DBR1_C.
DR   InterPro; IPR041816; Dbr1_N.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   Pfam; PF05011; DBR1; 1.
DR   Pfam; PF00149; Metallophos; 1.
DR   SMART; SM01124; DBR1; 1.
DR   SUPFAM; SSF56300; SSF56300; 1.
PE   3: Inferred from homology;
KW   Hydrolase; mRNA processing; Nucleus; Reference proteome.
FT   CHAIN           1..500
FT                   /note="Lariat debranching enzyme"
FT                   /id="PRO_0000250366"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          453..500
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        458..500
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   500 AA;  56682 MW;  DD12306B6EAD5609 CRC64;
     MSSKNPVDEQ PCCGSHEGSH QDPAPETLVV EKEVNNGEKK IRIAVVGCSH GEMDAIYETM
     TLIEQKNGYK FDLLICCGDY QAVRNYGDLP HMSIPPKYRS LQTFYKYYSG EQKAPVLTLF
     IGGNHEASGY LCELPNGGWV APNIYYMGFA NCIRFANLRI AGLSGIFSQG DFQFSHYERP
     SFSERDVKSA YHVRNVDMFR LRQLKSDNEN KISNPIDIML SHDWPGGIPD FGDKEWLFRK
     KDLFEADHNS GKLGNPSGMK LIYDCRPRYY LAAHLHIAFA ALVPHKGSGS GRPQPTRFLS
     LDKPIPGRKF MQALELNVAD DAKLELSYDP QWLAILRNTD LLTTGTKEQL ILPDMASNRP
     CVYERKDFRP TAEELKEIEK LGDLTIRTDS FQQTAPPLKE ITESSKNVPP SAYYRNPQSA
     EFCQWLGIRD LNQMLVEKTS EHVGTPYYMM TQDDANAKPN QDDVDFGDED FVIDRGHTSD
     EPEAKKSRLD EDKFEAVPSE