ID   DBR1_DROPS              Reviewed;         537 AA.
AC   Q29FE1;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 2.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Lariat debranching enzyme;
DE            EC=3.1.-.-;
GN   Name=DBR1; ORFNames=GA20707;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25 / Tucson 14011-0121.94;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA   Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA   Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA   Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA   Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA   Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA   Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT   gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- FUNCTION: Cleaves the 2'-5' phosphodiester linkage at the branch point
CC       of lariat intron pre-mRNAs after splicing and converts them into linear
CC       molecules that are subsequently degraded. It thereby facilitates
CC       ribonucleotide turnover. It may also participate in retrovirus
CC       replication via an RNA lariat intermediate in cDNA synthesis (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the lariat debranching enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; CH379067; EAL31314.2; -; Genomic_DNA.
DR   AlphaFoldDB; Q29FE1; -.
DR   SMR; Q29FE1; -.
DR   STRING; 7237.FBpp0277984; -.
DR   eggNOG; KOG2863; Eukaryota.
DR   HOGENOM; CLU_005893_0_0_1; -.
DR   InParanoid; Q29FE1; -.
DR   OMA; GIDDPLC; -.
DR   Proteomes; UP000001819; Genome assembly.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0008419; F:RNA lariat debranching enzyme activity; ISS:UniProtKB.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0000375; P:RNA splicing, via transesterification reactions; ISS:UniProtKB.
DR   CDD; cd00844; MPP_Dbr1_N; 1.
DR   Gene3D; 3.60.21.10; -; 1.
DR   InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR   InterPro; IPR007708; DBR1_C.
DR   InterPro; IPR041816; Dbr1_N.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   Pfam; PF05011; DBR1; 1.
DR   Pfam; PF00149; Metallophos; 1.
DR   SMART; SM01124; DBR1; 1.
DR   SUPFAM; SSF56300; SSF56300; 1.
PE   3: Inferred from homology;
KW   Hydrolase; mRNA processing; Nucleus; Reference proteome.
FT   CHAIN           1..537
FT                   /note="Lariat debranching enzyme"
FT                   /id="PRO_0000250368"
FT   REGION          242..272
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          473..537
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        244..262
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   537 AA;  59528 MW;  4A5B3458C0B6EE02 CRC64;
     MKIAIEGCAH GELERIYDTI ACIEKESNTK IDLLLCCGDF QSTRNLEDLQ TMAVPKKYLD
     ICTFYKYYSG ECVAPVLTIF IGGNHEASNY LQELPYGGWV APNIYYLGYA GVVNVNGVRI
     AGISGIYKGH DFLRGHHEFP PYTESTCRSV YHVRQLEVFR LKQLSGKIDI FLSHDWPTGI
     YEYGNKAQLL RKKPYFAADM ESGQLGSRPL EELLKAVQPS YWFAAHLHCK FAALVPHQNA
     TKAPTKMGDG SSSSSSSSSS ESDDEESTSR LPPKPVAVTK FLALDKCLPR RAFLQVLDIP
     SEAIEGNPTF EYDAEWLVIL QSTNHLISVK ENYYYLPGKK AGAIAERFNF TPTEEELDSL
     TTKFQSLKIP ENFQRTVPAF DPQEQSNYKH MVVGQPTAHL NPQSNTFCSV LGVDDPLCLA
     LLANGKDLPA VADQCQDQEP IEGSSPPPEP LVTPSKRKLN LFLPAPTVTA DATAAEKDDS
     VIDLPEEDED PKTAETAESE AVDNPKVKAV PPPPSSPPSV KKLKRRNQNI YQAEDDD