DBR1_SCHPO
ID DBR1_SCHPO Reviewed; 478 AA.
AC O13765; P78964;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 2.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Lariat debranching enzyme;
DE EC=3.1.-.-;
GN Name=dbr1; ORFNames=SPAC17A5.02c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9001235; DOI=10.1128/mcb.17.2.809;
RA Nam K., Lee G., Trambley J., Devine S.E., Boeke J.D.;
RT "Severe growth defect in a Schizosaccharomyces pombe mutant defective in
RT intron lariat degradation.";
RL Mol. Cell. Biol. 17:809-818(1997).
RN [2]
RP SEQUENCE REVISION.
RA Nam K.;
RL Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Cleaves the 2'-5' phosphodiester linkage at the branch point
CC of lariat intron pre-mRNAs after splicing and converts them into linear
CC molecules that are subsequently degraded. It thereby facilitates
CC ribonucleotide turnover (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the lariat debranching enzyme family.
CC {ECO:0000305}.
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DR EMBL; U63635; AAC49619.2; -; Genomic_DNA.
DR EMBL; CU329670; CAB11502.2; -; Genomic_DNA.
DR PIR; T37817; T37817.
DR RefSeq; NP_593470.2; NM_001018903.3.
DR AlphaFoldDB; O13765; -.
DR SMR; O13765; -.
DR BioGRID; 278746; 187.
DR STRING; 4896.SPAC17A5.02c.1; -.
DR iPTMnet; O13765; -.
DR MaxQB; O13765; -.
DR PaxDb; O13765; -.
DR PRIDE; O13765; -.
DR EnsemblFungi; SPAC17A5.02c.1; SPAC17A5.02c.1:pep; SPAC17A5.02c.
DR GeneID; 2542277; -.
DR KEGG; spo:SPAC17A5.02c; -.
DR PomBase; SPAC17A5.02c; dbr1.
DR VEuPathDB; FungiDB:SPAC17A5.02c; -.
DR eggNOG; KOG2863; Eukaryota.
DR HOGENOM; CLU_005893_1_0_1; -.
DR InParanoid; O13765; -.
DR OMA; ASNYMHE; -.
DR PhylomeDB; O13765; -.
DR PRO; PR:O13765; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005681; C:spliceosomal complex; IC:PomBase.
DR GO; GO:0008419; F:RNA lariat debranching enzyme activity; IGI:PomBase.
DR GO; GO:0031070; P:intronic snoRNA processing; ISO:PomBase.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IMP:PomBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd00844; MPP_Dbr1_N; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR007708; DBR1_C.
DR InterPro; IPR041816; Dbr1_N.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR Pfam; PF05011; DBR1; 1.
DR Pfam; PF00149; Metallophos; 1.
DR SMART; SM01124; DBR1; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
PE 3: Inferred from homology;
KW Hydrolase; mRNA processing; Nucleus; Reference proteome.
FT CHAIN 1..478
FT /note="Lariat debranching enzyme"
FT /id="PRO_0000079796"
FT REGION 250..324
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 258..272
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 277..323
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 478 AA; 54565 MW; 01629B8B8A10B9C7 CRC64;
MSYFARLKPP PAFLKMRVGV QGCCHGILDN LYILAEKRKV DLLIIGGDFQ ALRNVSDYHG
ISMPPKFKRL GDFFNYYNGR NKAPILTIFV GGNHEASNYL DELPYGGWVA PNIYYMGRSS
VINVGGLRIA GISGIYSAMD YKKGRYEGLP YNYKMLKSIY HTREFDVLSL KSLQKPIDIF
LSHDWPRGIE QHGDVAKLLR HKPFFRNEVE RNDLGSPALE ELLVELKPRY WMAAHLHTKF
TAVVHHNSQE DDGKLSCSSK DVTSSGFSMK GLNEPSQERL PVEKEQNDKS DEEGSNNEQE
EKQDKKQSTN RDLCRKESCK KEPSLSSSDQ VTKFLALDKC LPRRSYFEVV EIEPVEIPDS
GAPYMQYDSE WLSVLRAMHP FQSHTIEQDP PLPSLEVVKT LKRKEEIWVD ENLVKKDKLG
IPRNFCQTAP PHSRDITENM QPSSYINPQT VAFEILIGLK ERTVDSPPPV KNPNEIVL
