ID   DBR1_XENTR              Reviewed;         534 AA.
AC   Q6P886;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Lariat debranching enzyme;
DE            EC=3.1.-.-;
GN   Name=dbr1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cleaves the 2'-5' phosphodiester linkage at the branch point
CC       of excised lariat intron RNA and converts them into linear molecules
CC       that can be subsequently degraded, thereby facilitating ribonucleotide
CC       turnover. Linked to its role in pre-mRNA processing mechanism, may also
CC       participate in retrovirus replication and have an antiviral cell-
CC       intrinsic defense function. {ECO:0000250|UniProtKB:Q9UK59}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the lariat debranching enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; BC061343; AAH61343.1; -; mRNA.
DR   RefSeq; NP_988947.1; NM_203616.1.
DR   RefSeq; XP_012825690.1; XM_012970236.2.
DR   RefSeq; XP_012825691.1; XM_012970237.2.
DR   AlphaFoldDB; Q6P886; -.
DR   SMR; Q6P886; -.
DR   STRING; 8364.ENSXETP00000057335; -.
DR   PaxDb; Q6P886; -.
DR   Ensembl; ENSXETT00000057335; ENSXETP00000057335; ENSXETG00000027491.
DR   GeneID; 394544; -.
DR   KEGG; xtr:394544; -.
DR   CTD; 51163; -.
DR   Xenbase; XB-GENE-5851881; dbr1.
DR   eggNOG; KOG2863; Eukaryota.
DR   HOGENOM; CLU_005893_0_2_1; -.
DR   InParanoid; Q6P886; -.
DR   OMA; GIDDPLC; -.
DR   OrthoDB; 1047278at2759; -.
DR   PhylomeDB; Q6P886; -.
DR   TreeFam; TF313221; -.
DR   Proteomes; UP000008143; Chromosome 9.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000027491; Expressed in egg cell and 16 other tissues.
DR   ExpressionAtlas; Q6P886; differential.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0008419; F:RNA lariat debranching enzyme activity; ISS:UniProtKB.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   GO; GO:0000375; P:RNA splicing, via transesterification reactions; ISS:UniProtKB.
DR   CDD; cd00844; MPP_Dbr1_N; 1.
DR   Gene3D; 3.60.21.10; -; 1.
DR   InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR   InterPro; IPR007708; DBR1_C.
DR   InterPro; IPR041816; Dbr1_N.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   Pfam; PF05011; DBR1; 1.
DR   Pfam; PF00149; Metallophos; 1.
DR   SMART; SM01124; DBR1; 1.
DR   SUPFAM; SSF56300; SSF56300; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; mRNA processing; Nucleus; Reference proteome.
FT   CHAIN           1..534
FT                   /note="Lariat debranching enzyme"
FT                   /id="PRO_0000250364"
FT   REGION          388..457
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          470..534
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        409..424
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        427..441
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        480..503
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   534 AA;  60831 MW;  A2DA4252CA151592 CRC64;
     MKIAVEGCCH GELDKIYETI QFLEKKENTK VDLLLCCGDF QAVRNEGDMK CMAVPQKYRQ
     MQTFYKYYSG EKLAPILTIF IGGNHEASNY LQELPYGGWV APNIYYVGYA GVVKYRGVRI
     GGISGIFKSH DYRKGHFERP PYSKDTVRSA YHVRNIEVFR LKQLKEPMDI FMSHDWPRSI
     YHYGNKKQLL KKKDFFRQEV ENNTLGSPAA SELLLHIQPS YWFSAHLHVK FAAFMQHQTN
     VEGEIPKATK FLALDKCLPH REFLQIVDVE HDPGKPDCLE YDLEWLAVLK ATKDLLNITS
     KTWNMPENNG LHSRWDFSAS EKTKREILDD LGHDIKIPCN FCMTTACYDP NNPQYKRVAT
     HIVNPQTTEF CARLGLVDLN AKIRQHEEEG DIDITEDNEA DSIGSAEDPG EYSTDTSLLS
     TSINPDEIAL EDDDDQEDEG MAEKLGEPSP DYTPDLSINF SNIRVLPDSM AVSSDDATDS
     TNDELDRSES SQTEGEGKQS NRPLKRMSNE NGSGGVKIKR RNQAIYQAKD DEDE