ID   DCA12_CHICK             Reviewed;         427 AA.
AC   Q5F3R7;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=DDB1- and CUL4-associated factor 12 {ECO:0000305};
DE   AltName: Full=WD repeat-containing protein 40A;
GN   Name=DCAF12; Synonyms=WDR40A;
GN   ORFNames=RCJMB04_8j18 {ECO:0000303|PubMed:15642098};
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=CB; TISSUE=Bursa of Fabricius;
RX   PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA   Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA   Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA   Hayashizaki Y., Buerstedde J.-M.;
RT   "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT   function analysis.";
RL   Genome Biol. 6:R6.1-R6.9(2005).
CC   -!- FUNCTION: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3
CC       ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC       degrons) pathway, which recognizes a C-degron located at the extreme C
CC       terminus of target proteins, leading to their ubiquitination and
CC       degradation. The C-degron recognized by the DesCEND pathway is usually
CC       a motif of less than ten residues and can be present in full-length
CC       proteins, truncated proteins or proteolytically cleaved forms. The
CC       DCX(DCAF12) complex specifically recognizes proteins with a diglutamate
CC       (Glu-Glu) at the C-terminus, leading to their ubiquitination and
CC       degradation. {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SUBUNIT: Component of the DCX(DCAF12) E3 ubiquitin ligase complex, at
CC       least composed of CUL4 (CUL4A or CUL4B), DDB1, DCAF12 and RBX1.
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q5T6F0}.
CC       Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SIMILARITY: Belongs to the WD repeat DCAF12 family. {ECO:0000305}.
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DR   EMBL; AJ851583; CAH65217.1; -; mRNA.
DR   RefSeq; NP_001026615.1; NM_001031444.1.
DR   AlphaFoldDB; Q5F3R7; -.
DR   SMR; Q5F3R7; -.
DR   STRING; 9031.ENSGALP00000009301; -.
DR   PaxDb; Q5F3R7; -.
DR   Ensembl; ENSGALT00000009315; ENSGALP00000009301; ENSGALG00000005800.
DR   GeneID; 427396; -.
DR   KEGG; gga:427396; -.
DR   CTD; 340578; -.
DR   VEuPathDB; HostDB:geneid_427396; -.
DR   eggNOG; ENOG502QR7U; Eukaryota.
DR   GeneTree; ENSGT00940000158028; -.
DR   InParanoid; Q5F3R7; -.
DR   OMA; FDMCWLD; -.
DR   PhylomeDB; Q5F3R7; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q5F3R7; -.
DR   Proteomes; UP000000539; Chromosome Z.
DR   Bgee; ENSGALG00000005800; Expressed in colon and 13 other tissues.
DR   ExpressionAtlas; Q5F3R7; baseline and differential.
DR   GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR   GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0010506; P:regulation of autophagy; ISS:UniProtKB.
DR   GO; GO:0140627; P:ubiquitin-dependent protein catabolic process via the C-end degron rule pathway; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   SMART; SM00320; WD40; 2.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Cytoskeleton; Reference proteome; Repeat;
KW   Ubl conjugation pathway; WD repeat.
FT   CHAIN           1..427
FT                   /note="DDB1- and CUL4-associated factor 12"
FT                   /id="PRO_0000306843"
FT   REPEAT          110..150
FT                   /note="WD 1"
FT   REPEAT          154..192
FT                   /note="WD 2"
FT   REPEAT          222..261
FT                   /note="WD 3"
FT   REPEAT          310..347
FT                   /note="WD 4"
SQ   SEQUENCE   427 AA;  47618 MW;  14EA8B57A5590DFC CRC64;
     MARKTVSRKR KAAAASAAGP GGLYGEQYGW DHSVHKRKRL PPVKRSLVYY LKEREFHLGT
     LNKVFASQWL NHRQVVCGTK CNTLFVVDVQ TGQITKIPIL KDREPGMVNQ QGCGIHAIEL
     NPSRTLLATG GDNPNSLAIY RLPTLDPVCV GDDGHKDWIF SIAWISDTMA VSGSRDGSMG
     LWEVTEDVLS KSDSRHNLSQ VPVYAHITHR ALKDIPKENT NPDNCKVRAL AFNNKNKELG
     AVSLDGYFHL WKAEHTLSKL LSTKLPYCRE NVCLAYGQEW SVYAVGSQAH VSFLDPRQPS
     HNVKSVCSKE RGSGIRSVSF YEHIITVGTG HGSLLFYDIR AQRFLDERPP RACYGQKQKL
     GGSEILKLTT GKGWLNHDET WRNYFSEINF FPNAVYTHCY DSSGTKLFVA GGPLPSGLHG
     NYAGLWS