ID   DCA12_DANRE             Reviewed;         482 AA.
AC   Q08BB3;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=DDB1- and CUL4-associated factor 12 {ECO:0000305};
DE   AltName: Full=WD repeat-containing protein 40A;
GN   Name=dcaf12; Synonyms=wdr40a; ORFNames=zgc:154031;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3
CC       ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC       degrons) pathway, which recognizes a C-degron located at the extreme C
CC       terminus of target proteins, leading to their ubiquitination and
CC       degradation. The C-degron recognized by the DesCEND pathway is usually
CC       a motif of less than ten residues and can be present in full-length
CC       proteins, truncated proteins or proteolytically cleaved forms. The
CC       DCX(DCAF12) complex specifically recognizes proteins with a diglutamate
CC       (Glu-Glu) at the C-terminus, leading to their ubiquitination and
CC       degradation. {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SUBUNIT: Component of the DCX(DCAF12) E3 ubiquitin ligase complex, at
CC       least composed of cul4 (cul4a or cul4b), ddb1, dcaf12 and rbx1.
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q5T6F0}.
CC       Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SIMILARITY: Belongs to the WD repeat DCAF12 family. {ECO:0000305}.
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DR   EMBL; BC124796; AAI24797.1; -; mRNA.
DR   RefSeq; NP_001071048.1; NM_001077580.1.
DR   AlphaFoldDB; Q08BB3; -.
DR   SMR; Q08BB3; -.
DR   STRING; 7955.ENSDARP00000086448; -.
DR   PaxDb; Q08BB3; -.
DR   Ensembl; ENSDART00000092015; ENSDARP00000086448; ENSDARG00000063155.
DR   GeneID; 570671; -.
DR   KEGG; dre:570671; -.
DR   CTD; 25853; -.
DR   ZFIN; ZDB-GENE-061013-363; dcaf12.
DR   eggNOG; ENOG502QR7U; Eukaryota.
DR   GeneTree; ENSGT00940000158028; -.
DR   HOGENOM; CLU_020124_1_0_1; -.
DR   InParanoid; Q08BB3; -.
DR   OMA; FDMCWLD; -.
DR   OrthoDB; 885170at2759; -.
DR   PhylomeDB; Q08BB3; -.
DR   TreeFam; TF323731; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q08BB3; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 21.
DR   Bgee; ENSDARG00000063155; Expressed in testis and 21 other tissues.
DR   ExpressionAtlas; Q08BB3; baseline.
DR   GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR   GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0010506; P:regulation of autophagy; ISS:UniProtKB.
DR   GO; GO:0140627; P:ubiquitin-dependent protein catabolic process via the C-end degron rule pathway; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   SMART; SM00320; WD40; 2.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Cytoskeleton; Reference proteome; Repeat;
KW   Ubl conjugation pathway; WD repeat.
FT   CHAIN           1..482
FT                   /note="DDB1- and CUL4-associated factor 12"
FT                   /id="PRO_0000306844"
FT   REPEAT          167..207
FT                   /note="WD 1"
FT   REPEAT          211..249
FT                   /note="WD 2"
FT   REPEAT          279..318
FT                   /note="WD 3"
FT   REPEAT          367..404
FT                   /note="WD 4"
SQ   SEQUENCE   482 AA;  53871 MW;  63EE3461795684D1 CRC64;
     MARKTVSRKR KAEEPKEQQF DWCQSAFKRP RVSSSVRHAQ HWWQSRRSVV CSVRGREFRP
     QQQHEWSFQR SLRGFAAGRI PGILREREFS LGRLNKVFAS QWLNHRQVVC GTKCNTLFVV
     DVLSGQITRI PMLKDREGRG EVSQGLGGVG SPFHFHNPGS VGGLDVQQGC GIHAIELNPS
     RTLLATGGDN PNSLAVYRLP TLDPVCVGDD GHNDWIFSIA WISDTMAVSG SRDGSMGLWE
     ISEDVLSQAE KRQNVEGVPG YSHISHRALK DIPKEYTHPY NCKVRALAFN NSHKELGAVS
     LDGYFHLWKA EDNLSKELST KLPYCKENVC LAYGLDWSVY AVGSQAHVSF LDPRQSSQNI
     KSVSSRERGS GIRSVSFYEH IVTVGTGQGS LLFYDIRAQR FLDGPSSTPG GYRNRTAEGI
     LKLTTGRGWL NHDETWRSYF SDIRSFPNAV YTHCYDDSGT KLFVAGGPLC SGLHGNYAGL
     WS