ID   DCA12_MACFA             Reviewed;         453 AA.
AC   Q4R3J7;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=DDB1- and CUL4-associated factor 12 {ECO:0000305};
DE   AltName: Full=WD repeat-containing protein 40A;
GN   Name=DCAF12; Synonyms=WDR40A; ORFNames=QtsA-16482;
OS   Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   International consortium for macaque cDNA sequencing and analysis;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3
CC       ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC       degrons) pathway, which recognizes a C-degron located at the extreme C
CC       terminus of target proteins, leading to their ubiquitination and
CC       degradation. The C-degron recognized by the DesCEND pathway is usually
CC       a motif of less than ten residues and can be present in full-length
CC       proteins, truncated proteins or proteolytically cleaved forms. The
CC       DCX(DCAF12) complex specifically recognizes proteins with a diglutamate
CC       (Glu-Glu) at the C-terminus, such as MAGEA3, MAGEA6 and CCT5, leading
CC       to their ubiquitination and degradation. Ubiquitination of MAGEA3,
CC       MAGEA6 by DCX(DCAF12) complex is required for starvation-induced
CC       autophagy. {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SUBUNIT: Component of the DCX(DCAF12) E3 ubiquitin ligase complex, at
CC       least composed of CUL4 (CUL4A or CUL4B), DDB1, DCAF12 and RBX1.
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q5T6F0}.
CC       Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SIMILARITY: Belongs to the WD repeat DCAF12 family. {ECO:0000305}.
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DR   EMBL; AB179269; BAE02320.1; -; mRNA.
DR   RefSeq; NP_001271872.1; NM_001284943.1.
DR   AlphaFoldDB; Q4R3J7; -.
DR   SMR; Q4R3J7; -.
DR   STRING; 9541.XP_005581555.1; -.
DR   Ensembl; ENSMFAT00000009742; ENSMFAP00000035503; ENSMFAG00000036190.
DR   GeneID; 101925316; -.
DR   CTD; 25853; -.
DR   VEuPathDB; HostDB:ENSMFAG00000036190; -.
DR   eggNOG; ENOG502QR7U; Eukaryota.
DR   GeneTree; ENSGT00940000158028; -.
DR   OMA; FDMCWLD; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000233100; Chromosome 15.
DR   Bgee; ENSMFAG00000036190; Expressed in bone marrow and 13 other tissues.
DR   GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR   GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0010506; P:regulation of autophagy; ISS:UniProtKB.
DR   GO; GO:0140627; P:ubiquitin-dependent protein catabolic process via the C-end degron rule pathway; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   SMART; SM00320; WD40; 4.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome; Repeat;
KW   Ubl conjugation pathway; WD repeat.
FT   CHAIN           1..453
FT                   /note="DDB1- and CUL4-associated factor 12"
FT                   /id="PRO_0000306841"
FT   REPEAT          138..178
FT                   /note="WD 1"
FT   REPEAT          182..220
FT                   /note="WD 2"
FT   REPEAT          250..289
FT                   /note="WD 3"
FT   REPEAT          338..375
FT                   /note="WD 4"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         15
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q5T6F0"
SQ   SEQUENCE   453 AA;  50507 MW;  8784ADAED11C32C6 CRC64;
     MARKAVSRKR KAPASPGAGS DAQGQQFGWD HTLHKRKRLP PVKRSLVYYL KNREVRLQNE
     TSYSRVLHGY AAQQLPSLLK EREFHLGTLN KVFASQWLNH RQVVCGTKCN TLFVVDVQTS
     QITKIPILKD REPGGVTQQG CGIHAIELNP SRTLLATGGD NPNSLAIYRL PTLDPVCVGD
     DGHKDWIFSI AWISDTMAVS GSRDGSMGLW EVTDDVLTKS DARHNVSRVP VYAHITHKAL
     KDIPKEDTNP DNCKVRALAF NSKNKELGAV SLDGYFHLWK AENTLSKLLS TKLPYCRENV
     CLAYGSEWSV YAVGSQAHVS FLDPRQPSYN VKSVCSRERG SGIRSVSFYE HIITVGTGQG
     SLLFYDIRAQ RFLEERLSAC YGSKPRLAGE NLKLTTGKGW LNHDETWRNY FSDIDFFPNA
     VYTHCYDSSG TKLFVAGGPL PSGLHGNYAG LWS