DCA12_MOUSE
ID DCA12_MOUSE Reviewed; 453 AA.
AC Q8BGZ3; Q69Z77; Q8BN60; Q8C1Y4; Q9DB95;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=DDB1- and CUL4-associated factor 12 {ECO:0000305};
DE AltName: Full=WD repeat-containing protein 40A;
GN Name=Dcaf12 {ECO:0000312|MGI:MGI:1916220};
GN Synonyms=Kiaa1892 {ECO:0000303|PubMed:15368895}, Wdr40a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Fetal brain;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, Eye, Forelimb, Kidney, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3
CC ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC degrons) pathway, which recognizes a C-degron located at the extreme C
CC terminus of target proteins, leading to their ubiquitination and
CC degradation. The C-degron recognized by the DesCEND pathway is usually
CC a motif of less than ten residues and can be present in full-length
CC proteins, truncated proteins or proteolytically cleaved forms. The
CC DCX(DCAF12) complex specifically recognizes proteins with a diglutamate
CC (Glu-Glu) at the C-terminus, such as MAGEA3, MAGEA6 and CCT5, leading
CC to their ubiquitination and degradation. Ubiquitination of MAGEA3,
CC MAGEA6 by DCX(DCAF12) complex is required for starvation-induced
CC autophagy. {ECO:0000250|UniProtKB:Q5T6F0}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:Q5T6F0}.
CC -!- SUBUNIT: Component of the DCX(DCAF12) E3 ubiquitin ligase complex, at
CC least composed of CUL4 (CUL4A or CUL4B), DDB1, DCAF12 and RBX1.
CC {ECO:0000250|UniProtKB:Q5T6F0}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q5T6F0}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC {ECO:0000250|UniProtKB:Q5T6F0}.
CC -!- SIMILARITY: Belongs to the WD repeat DCAF12 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC39908.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAD32567.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK173289; BAD32567.1; ALT_INIT; mRNA.
DR EMBL; AK005099; BAB23817.2; -; mRNA.
DR EMBL; AK030173; BAC26822.1; -; mRNA.
DR EMBL; AK031089; BAC27247.1; -; mRNA.
DR EMBL; AK087517; BAC39908.1; ALT_FRAME; mRNA.
DR EMBL; AK090028; BAC41053.1; -; mRNA.
DR EMBL; AL807823; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC023852; AAH23852.1; -; mRNA.
DR CCDS; CCDS18059.1; -.
DR RefSeq; NP_081169.2; NM_026893.3.
DR AlphaFoldDB; Q8BGZ3; -.
DR SMR; Q8BGZ3; -.
DR BioGRID; 213148; 1.
DR STRING; 10090.ENSMUSP00000030145; -.
DR iPTMnet; Q8BGZ3; -.
DR PhosphoSitePlus; Q8BGZ3; -.
DR EPD; Q8BGZ3; -.
DR MaxQB; Q8BGZ3; -.
DR PaxDb; Q8BGZ3; -.
DR PeptideAtlas; Q8BGZ3; -.
DR PRIDE; Q8BGZ3; -.
DR ProteomicsDB; 279160; -.
DR Antibodypedia; 2916; 138 antibodies from 25 providers.
DR DNASU; 68970; -.
DR Ensembl; ENSMUST00000030145; ENSMUSP00000030145; ENSMUSG00000028436.
DR GeneID; 68970; -.
DR KEGG; mmu:68970; -.
DR UCSC; uc008sil.2; mouse.
DR CTD; 25853; -.
DR MGI; MGI:1916220; Dcaf12.
DR VEuPathDB; HostDB:ENSMUSG00000028436; -.
DR eggNOG; ENOG502QR7U; Eukaryota.
DR GeneTree; ENSGT00940000158028; -.
DR HOGENOM; CLU_020124_1_0_1; -.
DR InParanoid; Q8BGZ3; -.
DR OMA; FDMCWLD; -.
DR OrthoDB; 885170at2759; -.
DR PhylomeDB; Q8BGZ3; -.
DR TreeFam; TF323731; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 68970; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Dcaf12; mouse.
DR PRO; PR:Q8BGZ3; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8BGZ3; protein.
DR Bgee; ENSMUSG00000028436; Expressed in blood and 263 other tissues.
DR Genevisible; Q8BGZ3; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0010506; P:regulation of autophagy; ISS:UniProtKB.
DR GO; GO:0140627; P:ubiquitin-dependent protein catabolic process via the C-end degron rule pathway; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome; Repeat;
KW Ubl conjugation pathway; WD repeat.
FT CHAIN 1..453
FT /note="DDB1- and CUL4-associated factor 12"
FT /id="PRO_0000306842"
FT REPEAT 138..178
FT /note="WD 1"
FT REPEAT 182..220
FT /note="WD 2"
FT REPEAT 250..289
FT /note="WD 3"
FT REPEAT 338..375
FT /note="WD 4"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 15
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5T6F0"
FT CONFLICT 44
FT /note="R -> S (in Ref. 1; BAD32567)"
FT /evidence="ECO:0000305"
FT CONFLICT 294
FT /note="P -> A (in Ref. 2; BAC39908)"
FT /evidence="ECO:0000305"
FT CONFLICT 375
FT /note="E -> G (in Ref. 2; BAC41053)"
FT /evidence="ECO:0000305"
FT CONFLICT 438
FT /note="G -> P (in Ref. 2; BAB23817)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 453 AA; 50459 MW; 60212662F05FAB7A CRC64;
MARKAVSRKR KASASPGAGS DAQGPQFGWD HSLHKRKRLP PVKRSLVYYL KNREVRLQNE
TSYSRLLHGY AAQQLPSLLK EREFHLGTLN KVFASQWLNH RQVVCGTKCN TLFVVDVQTG
QITKIPILKD REPGGVTQQG CGIHAIELNP SRTLLATGGD NPNSLAIYRL PTLDPVCVGD
DGHKDWIFSI AWINDTMAVS GSRDGSMGLW EVTDDVLTKS DARHNVSPVP VYAHITHKAL
KDIPKEDTNP DNCKVRALAF NNKNKELGAV SLDGYFHLWK AENTLSKLLS TKLPYCRENV
CLAYGSEWSV YAVGSQAHVS FLDPRQPSYN VKSVCSRERG SGIRSVSFYE HIITVGTGQG
SLLFYDIRAQ RFLEERLSAC YGSKPRLAGE NLKLTTGRGW LNHDETWRNY FSDIDFFPNA
VYTHCYDSSG TKLFVAGGPL PSGLHGNYAG LWS
