ID   DCA12_XENTR             Reviewed;         446 AA.
AC   Q6P809;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=DDB1- and CUL4-associated factor 12 {ECO:0000305};
DE   AltName: Full=WD repeat-containing protein 40A;
GN   Name=dcaf12 {ECO:0000250|UniProtKB:Q5T6F0}; Synonyms=wdr40a;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3
CC       ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC       degrons) pathway, which recognizes a C-degron located at the extreme C
CC       terminus of target proteins, leading to their ubiquitination and
CC       degradation. The C-degron recognized by the DesCEND pathway is usually
CC       a motif of less than ten residues and can be present in full-length
CC       proteins, truncated proteins or proteolytically cleaved forms. The
CC       DCX(DCAF12) complex specifically recognizes proteins with a diglutamate
CC       (Glu-Glu) at the C-terminus, leading to their ubiquitination and
CC       degradation. {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SUBUNIT: Component of the DCX(DCAF12) E3 ubiquitin ligase complex, at
CC       least composed of cul4 (cul4a or cul4b), ddb1, dcaf12 and rbx1.
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q5T6F0}.
CC       Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC       {ECO:0000250|UniProtKB:Q5T6F0}.
CC   -!- SIMILARITY: Belongs to the WD repeat DCAF12 family. {ECO:0000305}.
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DR   EMBL; BC061422; AAH61422.1; -; mRNA.
DR   RefSeq; NP_988998.1; NM_203667.1.
DR   AlphaFoldDB; Q6P809; -.
DR   SMR; Q6P809; -.
DR   DNASU; 394594; -.
DR   GeneID; 394594; -.
DR   KEGG; xtr:394594; -.
DR   CTD; 25853; -.
DR   Xenbase; XB-GENE-1008710; dcaf12.
DR   InParanoid; Q6P809; -.
DR   OrthoDB; 885170at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000008143; Chromosome 1.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR   GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0010506; P:regulation of autophagy; ISS:UniProtKB.
DR   GO; GO:0140627; P:ubiquitin-dependent protein catabolic process via the C-end degron rule pathway; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Cytoskeleton; Reference proteome; Repeat;
KW   Ubl conjugation pathway; WD repeat.
FT   CHAIN           1..446
FT                   /note="DDB1- and CUL4-associated factor 12"
FT                   /id="PRO_0000306847"
FT   REPEAT          132..173
FT                   /note="WD 1"
FT   REPEAT          177..215
FT                   /note="WD 2"
FT   REPEAT          245..284
FT                   /note="WD 3"
FT   REPEAT          333..370
FT                   /note="WD 4"
FT   REGION          1..34
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   446 AA;  49514 MW;  BA65BB6F2F04A8EE CRC64;
     MTRRAVSRKR RAAPGTGPGE QSDWDHSAHK RKRLPPEKKS LVFYLKSREL KPHNDSSYLR
     LLRGHAACTL PGILSEREFH LGTLNKVFAS QWLNHRQVVC GTKCNTLFVV DVQTGQITKI
     PILKDREPLS GSHQSCGIHA IEINPSRTLL ATGGDNPNSI AVYRLPTLDP VCVGDGGHND
     WIFSIAWISD TMAVSGSRDG SMALWEMTEE ILSKSDFQHG LSRVPVYAHI SHKALKDIPK
     ESSNPVNCKV RALAFNSKNK ELGAVSLDGF FHLWKAELTL AKLLSTKLPY CRENVCLAYG
     LEWSLYAVGS QAHVSFLDPR QPPQCAKSVY CREQGSGIRS VSFYEHIVTV GTGQGALLFY
     DIRAQRFLED STGSCRNSKY KGDLLKLSTG KGWLNHNEMW MNYFSDIDCC PNAVYTHCYD
     SSGTKLFVAG GPLPTGLHGN YAGLWS