DCA16_HUMAN
ID DCA16_HUMAN Reviewed; 216 AA.
AC Q9NXF7; B3KPB7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=DDB1- and CUL4-associated factor 16 {ECO:0000305};
GN Name=DCAF16 {ECO:0000312|HGNC:HGNC:25987}; Synonyms=C4orf30;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart, Kidney, Lung, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, INTERACTION WITH DDB1 AND CUL4A, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=16949367; DOI=10.1016/j.molcel.2006.08.010;
RA Jin J., Arias E.E., Chen J., Harper J.W., Walter J.C.;
RT "A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is
RT required for S phase destruction of the replication factor Cdt1.";
RL Mol. Cell 23:709-721(2006).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-61, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=31209349; DOI=10.1038/s41589-019-0279-5;
RA Zhang X., Crowley V.M., Wucherpfennig T.G., Dix M.M., Cravatt B.F.;
RT "Electrophilic PROTACs that degrade nuclear proteins by engaging DCAF16.";
RL Nat. Chem. Biol. 15:737-746(2019).
CC -!- FUNCTION: Functions as a substrate recognition component for CUL4-DDB1
CC E3 ubiquitin-protein ligase complex, which mediates ubiquitination and
CC proteasome-dependent degradation of nuclear proteins.
CC {ECO:0000269|PubMed:16949367, ECO:0000269|PubMed:31209349}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Interacts with DDB1 and CUL4A. {ECO:0000269|PubMed:16949367}.
CC -!- INTERACTION:
CC Q9NXF7; P04608: tat; Xeno; NbExp=2; IntAct=EBI-2559096, EBI-6164389;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31209349}.
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DR EMBL; AK000287; BAA91056.1; -; mRNA.
DR EMBL; AK056116; BAG51629.1; -; mRNA.
DR EMBL; CH471069; EAW92784.1; -; Genomic_DNA.
DR EMBL; BC050697; AAH50697.1; -; mRNA.
DR EMBL; BC068025; AAH68025.1; -; mRNA.
DR EMBL; BC101716; AAI01717.1; -; mRNA.
DR EMBL; BC101718; AAI01719.1; -; mRNA.
DR CCDS; CCDS3423.1; -.
DR RefSeq; NP_001332809.1; NM_001345880.1.
DR RefSeq; NP_001332810.1; NM_001345881.1.
DR RefSeq; NP_001332811.1; NM_001345882.1.
DR RefSeq; NP_001332813.1; NM_001345884.1.
DR RefSeq; NP_001332814.1; NM_001345885.1.
DR RefSeq; NP_060211.3; NM_017741.3.
DR RefSeq; XP_016863816.1; XM_017008327.1.
DR RefSeq; XP_016863817.1; XM_017008328.1.
DR AlphaFoldDB; Q9NXF7; -.
DR BioGRID; 120224; 61.
DR IntAct; Q9NXF7; 25.
DR MINT; Q9NXF7; -.
DR STRING; 9606.ENSP00000371682; -.
DR iPTMnet; Q9NXF7; -.
DR PhosphoSitePlus; Q9NXF7; -.
DR BioMuta; DCAF16; -.
DR DMDM; 74719452; -.
DR EPD; Q9NXF7; -.
DR jPOST; Q9NXF7; -.
DR MassIVE; Q9NXF7; -.
DR MaxQB; Q9NXF7; -.
DR PaxDb; Q9NXF7; -.
DR PeptideAtlas; Q9NXF7; -.
DR PRIDE; Q9NXF7; -.
DR ProteomicsDB; 83088; -.
DR Antibodypedia; 51178; 16 antibodies from 9 providers.
DR DNASU; 54876; -.
DR Ensembl; ENST00000382247.6; ENSP00000371682.1; ENSG00000163257.11.
DR GeneID; 54876; -.
DR KEGG; hsa:54876; -.
DR MANE-Select; ENST00000382247.6; ENSP00000371682.1; NM_017741.4; NP_060211.3.
DR UCSC; uc003gpn.3; human.
DR CTD; 54876; -.
DR DisGeNET; 54876; -.
DR GeneCards; DCAF16; -.
DR HGNC; HGNC:25987; DCAF16.
DR HPA; ENSG00000163257; Low tissue specificity.
DR neXtProt; NX_Q9NXF7; -.
DR OpenTargets; ENSG00000163257; -.
DR PharmGKB; PA165663579; -.
DR VEuPathDB; HostDB:ENSG00000163257; -.
DR eggNOG; ENOG502RC7Y; Eukaryota.
DR GeneTree; ENSGT00390000012430; -.
DR HOGENOM; CLU_111172_0_0_1; -.
DR InParanoid; Q9NXF7; -.
DR OMA; SGQEWDS; -.
DR OrthoDB; 1225738at2759; -.
DR PhylomeDB; Q9NXF7; -.
DR TreeFam; TF341783; -.
DR PathwayCommons; Q9NXF7; -.
DR Reactome; R-HSA-8951664; Neddylation.
DR SignaLink; Q9NXF7; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 54876; 7 hits in 1103 CRISPR screens.
DR ChiTaRS; DCAF16; human.
DR GenomeRNAi; 54876; -.
DR Pharos; Q9NXF7; Tdark.
DR PRO; PR:Q9NXF7; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q9NXF7; protein.
DR Bgee; ENSG00000163257; Expressed in calcaneal tendon and 190 other tissues.
DR Genevisible; Q9NXF7; HS.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR InterPro; IPR028216; DCAF16.
DR PANTHER; PTHR16194; PTHR16194; 1.
DR Pfam; PF15349; DCA16; 1.
PE 1: Evidence at protein level;
KW Acetylation; Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..216
FT /note="DDB1- and CUL4-associated factor 16"
FT /id="PRO_0000301964"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..39
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 61
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:19608861"
FT VARIANT 45
FT /note="N -> S (in dbSNP:rs34085539)"
FT /id="VAR_034917"
FT VARIANT 129
FT /note="T -> I (in dbSNP:rs7690457)"
FT /id="VAR_034918"
FT CONFLICT 181
FT /note="W -> R (in Ref. 1; BAG51629)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 216 AA; 24193 MW; D34295DCBC579986 CRC64;
MGPRNPSPDH LSESESEEEE NISYLNESSG EEWDSSEEED SMVPNLSPLE SLAWQVKCLL
KYSTTWKPLN PNSWLYHAKL LDPSTPVHIL REIGLRLSHC SHCVPKLEPI PEWPPLASCG
VPPFQKPLTS PSRLSRDHAT LNGALQFATK QLSRTLSRAT PIPEYLKQIP NSCVSGCCCG
WLTKTVKETT RTEPINTTYS YTDFQKAVNK LLTASL
