DCAF5_MOUSE
ID DCAF5_MOUSE Reviewed; 946 AA.
AC Q80T85; Q80VT3; Q80ZW6; Q8BIP8; Q8BVK5; Q8K0Q6;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=DDB1- and CUL4-associated factor 5;
DE AltName: Full=WD repeat-containing protein 22;
GN Name=Dcaf5; Synonyms=Kiaa1824, Wdr22;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, Eye, and Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 415-946.
RC STRAIN=C57BL/6J; TISSUE=Embryonic testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-500; SER-531; SER-533;
RP SER-626 AND SER-628, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May function as a substrate receptor for CUL4-DDB1 E3
CC ubiquitin-protein ligase complex. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Interacts with DDB1 and CUL4A. {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC65843.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK122561; BAC65843.1; ALT_INIT; mRNA.
DR EMBL; BC030857; AAH30857.1; -; mRNA.
DR EMBL; BC042567; AAH42567.1; -; mRNA.
DR EMBL; BC046813; AAH46813.2; -; mRNA.
DR EMBL; AK034653; BAC28784.1; -; mRNA.
DR EMBL; AK077889; BAC37050.1; -; mRNA.
DR CCDS; CCDS26013.1; -.
DR RefSeq; NP_796241.3; NM_177267.4.
DR AlphaFoldDB; Q80T85; -.
DR SMR; Q80T85; -.
DR BioGRID; 236308; 10.
DR IntAct; Q80T85; 1.
DR STRING; 10090.ENSMUSP00000052755; -.
DR iPTMnet; Q80T85; -.
DR PhosphoSitePlus; Q80T85; -.
DR SwissPalm; Q80T85; -.
DR EPD; Q80T85; -.
DR MaxQB; Q80T85; -.
DR PaxDb; Q80T85; -.
DR PRIDE; Q80T85; -.
DR ProteomicsDB; 279882; -.
DR Antibodypedia; 54896; 17 antibodies from 9 providers.
DR DNASU; 320808; -.
DR Ensembl; ENSMUST00000054145; ENSMUSP00000052755; ENSMUSG00000049106.
DR GeneID; 320808; -.
DR KEGG; mmu:320808; -.
DR UCSC; uc007oar.2; mouse.
DR CTD; 8816; -.
DR MGI; MGI:2444785; Dcaf5.
DR VEuPathDB; HostDB:ENSMUSG00000049106; -.
DR eggNOG; KOG4227; Eukaryota.
DR GeneTree; ENSGT00950000182900; -.
DR HOGENOM; CLU_018663_0_0_1; -.
DR InParanoid; Q80T85; -.
DR OMA; AGMREDP; -.
DR OrthoDB; 335761at2759; -.
DR PhylomeDB; Q80T85; -.
DR TreeFam; TF320710; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 320808; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Dcaf5; mouse.
DR PRO; PR:Q80T85; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q80T85; protein.
DR Bgee; ENSMUSG00000049106; Expressed in supraoptic nucleus and 224 other tissues.
DR ExpressionAtlas; Q80T85; baseline and differential.
DR Genevisible; Q80T85; MM.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR045151; DCAF8.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR15574; PTHR15574; 1.
DR Pfam; PF00400; WD40; 3.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome; Repeat; Ubl conjugation pathway;
KW WD repeat.
FT CHAIN 1..946
FT /note="DDB1- and CUL4-associated factor 5"
FT /id="PRO_0000051370"
FT REPEAT 51..91
FT /note="WD 1"
FT REPEAT 99..139
FT /note="WD 2"
FT REPEAT 140..180
FT /note="WD 3"
FT REPEAT 185..225
FT /note="WD 4"
FT REPEAT 277..317
FT /note="WD 5"
FT REPEAT 331..370
FT /note="WD 6"
FT REGION 449..478
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 527..656
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 675..860
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 894..946
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 449..469
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 554..569
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 625..645
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 683..697
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 698..747
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 756..774
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 803..819
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 922..946
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 500
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 531
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 533
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 626
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 628
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 645
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96JK2"
FT CONFLICT 509
FT /note="R -> L (in Ref. 3; BAC28784)"
FT /evidence="ECO:0000305"
FT CONFLICT 529..538
FT /note="NESDSEENVC -> RTRGSTHASG (in Ref. 2; AAH30857)"
FT /evidence="ECO:0000305"
FT CONFLICT 694
FT /note="A -> T (in Ref. 3; BAC28784)"
FT /evidence="ECO:0000305"
FT CONFLICT 788..793
FT /note="ALSSRA -> GREQSG (in Ref. 3; BAC28784)"
FT /evidence="ECO:0000305"
FT CONFLICT 798
FT /note="A -> T (in Ref. 2; AAH30857)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 946 AA; 103676 MW; 554778AFC28E4EB3 CRC64;
MKRRAGLGGS MRSVVGFLSQ RGLHGDPLLT QDFQRRRLRG CRNLYKKDLL GHFGCVNAIE
FSNNGGQWLV SGGDDRRVLL WHMEQAIHSR VKPIQLKGEH HSNIFCLAFN SGNTKVFSGG
NDEQVILHDV ESSETLDVFA HEDAVYGLSV SPVNDNIFAS SSDDGRVLIW DIRESPHGEP
FCLANYPSAF HSVMFNPVEP RLLATANSKE GVGLWDIRKP QSSLLRYGGN LSLQSAMSVR
FNSNGTQLLA LRRRLPPVLY DIHSRLPVFQ FDNQGYFNSC TMKSCCFAGD RDQYILSGSD
DFNLYMWKIP ADPEAGGIGR VVNGAFMVLK GHRSIVNQVR FNPHTYMICS SGVEKIIKIW
SPYKQPGCTG DLDGRIEDDS RCLYTHEEYI SLVLNSGSGL SHDYANQSVQ EDPRMMAFFD
SLVRREIEGW SSDSDSDLSE STILQLHAGV SERSGYTDSE SSASLPRSPP PTVDESADNA
FHLGPLRVTT TNAVASTPAT PTCEDASSRQ QRLSALRRYQ DKRLLALSNE SDSEENVCEA
ELDTDLFPRP RSPSPEDGSS SPSSSTSSED EEELNQRRAT TRQRNAMRRR QKTARDERPT
GPAKSTNTYI GEDNYDYPQI KVDDLSPSPD SSPERSASTL DVQPSRAPPA ANMESVERKI
YKAYKWLRCS YISYSNNKDG ETSLMAEEAD EGRAGTSHKD NPTPSSSKEA CLTATAQRDQ
DLPSEGCSSD ACKEGTSAGN PNSGAGHEHS SHPWAEVPEG TSQDTNNSGP LEHSFETKKL
NGKALSKALS SRAEEPPASP GPKASGSTLN SASGNCPRTQ SDDSEERSLD TVCANHNNGR
LHPRPLHPHN NGQSSGELET VACSSPGHLD TDHDSPSLTG TFLHKDCCGS EMACETPSAG
MREDPPDPSG LDSSKVVHGQ SGLKRHRIEL EDTESENSSS EKKLKT
