ID   DCAF8_PONAB             Reviewed;         597 AA.
AC   Q5R448;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=DDB1- and CUL4-associated factor 8;
DE   AltName: Full=WD repeat-containing protein 42A;
GN   Name=DCAF8; Synonyms=WDR42A;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May function as a substrate receptor for CUL4-DDB1 E3
CC       ubiquitin-protein ligase complex. {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Interacts with DDB1, CUL4A and CUL4B. Interacts with KPNA1,
CC       KPNB1 and XPO1. {ECO:0000250, ECO:0000250|UniProtKB:Q5TAQ9}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q5TAQ9}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q5TAQ9}. Note=It shuttles between the nucleus
CC       and the cytoplasm. Nuclear import is mediated by KPNA1 and KPNB1 under
CC       the regulation of nuclear GTPase RAN. Nuclear export to the cytoplasm
CC       is XPO1 dependent. {ECO:0000250|UniProtKB:Q5TAQ9}.
CC   -!- SIMILARITY: Belongs to the WD repeat DCAF8 family. {ECO:0000305}.
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DR   EMBL; CR861411; CAH93468.1; -; mRNA.
DR   RefSeq; NP_001127032.1; NM_001133560.1.
DR   AlphaFoldDB; Q5R448; -.
DR   SMR; Q5R448; -.
DR   STRING; 9601.ENSPPYP00000000744; -.
DR   GeneID; 100174058; -.
DR   KEGG; pon:100174058; -.
DR   CTD; 50717; -.
DR   eggNOG; KOG1334; Eukaryota.
DR   InParanoid; Q5R448; -.
DR   OrthoDB; 1270484at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR045151; DCAF8.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR15574; PTHR15574; 1.
DR   Pfam; PF00400; WD40; 3.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Methylation; Nucleus; Phosphoprotein; Reference proteome;
KW   Repeat; Ubl conjugation pathway; WD repeat.
FT   CHAIN           1..597
FT                   /note="DDB1- and CUL4-associated factor 8"
FT                   /id="PRO_0000296959"
FT   REPEAT          191..230
FT                   /note="WD 1"
FT   REPEAT          234..275
FT                   /note="WD 2"
FT   REPEAT          281..321
FT                   /note="WD 3"
FT   REPEAT          329..369
FT                   /note="WD 4"
FT   REPEAT          385..424
FT                   /note="WD 5"
FT   REPEAT          432..472
FT                   /note="WD 6"
FT   REPEAT          476..515
FT                   /note="WD 7"
FT   REGION          1..147
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          558..597
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           39..50
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250|UniProtKB:Q5TAQ9"
FT   MOTIF           114..122
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250|UniProtKB:Q5TAQ9"
FT   COMPBIAS        1..25
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        51..67
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        68..97
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        112..139
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         21
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N7N5"
FT   MOD_RES         22
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N7N5"
FT   MOD_RES         99
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N7N5"
FT   MOD_RES         129
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N7N5"
FT   MOD_RES         130
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N7N5"
FT   MOD_RES         204
FT                   /note="Omega-N-methylarginine; by PRMT1"
FT                   /evidence="ECO:0000250|UniProtKB:Q5TAQ9"
SQ   SEQUENCE   597 AA;  66903 MW;  E87E4F38B15BFF5B CRC64;
     MSSKGSSTDG RTDLANGSLS SSPEEMSGAE EGRETSSGIE VEASDLSLSL TGDDGGPNRT
     STESRGTDTE SSGEDKDSDS MEDTGHYSIN DENRVHDRSE EEEEEEEEEE EEQPRRRVQR
     KRANRDQDSS DDERALEDWV SSETSALPRP RWQALPALRE RELGSSARFV YEACGARVFV
     QRFRLQHGLE GHTGCVNTLH FNQRGTWLAS GSDDLKVVVW DWVRRQPVLD FESGHKSNVF
     QAKFLPNSGD STLAMRARDG QVRVAELSAT QCCKNTKRVA QHKGASHKLA LEPDSPCTFL
     SAGEDAVVFT IDLRQDRPAS KLVVTKEKEK KVGLYTIYVN PANTHQFAVG GRDQFVRIYD
     QRKIDENENN GVLKKFCPHH LVNGESKANI TCLVYSHDGT ELLASYNDED IYLFNSSHSD
     GAQYVKRYKG HRNNATVKGV NFYGPKSEFV VSGSDCGHIF LWEKSSCQII QFMEGDKGGV
     VNCLEPHPHL PVLATSGLDH DVKIWAPTAE ASTELTGLKD VIKKNKRERD EDSLHRTDLF
     DSHMLWFLMH HLRQRRHHRR WREPGVGATD ADSDESPSSS DTSDEEEGPD RVQCMPS