ID   DCD3A_DICDI             Reviewed;         288 AA.
AC   Q6TMJ1; Q54J41;
DT   19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Putative alkaline ceramidase dcd3A;
DE            EC=3.5.1.-;
GN   Name=dcd3A; ORFNames=DDB_G0288359;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND INDUCTION BY
RP   SRFA.
RX   PubMed=15075287; DOI=10.1128/ec.3.2.564-566.2004;
RA   Escalante R., Iranfar N., Sastre L., Loomis W.F.;
RT   "Identification of genes dependent on the MADS box transcription factor
RT   SrfA in Dictyostelium discoideum development.";
RL   Eukaryot. Cell 3:564-566(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Expressed early in development at low levels and
CC       up-regulated late in development. The high levels during culmination is
CC       dependent on srfA. The late induction after 24 hours of development was
CC       not observed in srfA-null strains. {ECO:0000269|PubMed:15075287}.
CC   -!- INDUCTION: Up-regulated by srfA transcription factor.
CC       {ECO:0000269|PubMed:15075287}.
CC   -!- SIMILARITY: Belongs to the alkaline ceramidase family. {ECO:0000305}.
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DR   EMBL; AY392442; AAQ98884.1; -; Genomic_DNA.
DR   EMBL; AAFI02000111; EAL63276.1; -; Genomic_DNA.
DR   RefSeq; XP_636760.1; XM_631668.1.
DR   AlphaFoldDB; Q6TMJ1; -.
DR   SMR; Q6TMJ1; -.
DR   STRING; 44689.DDB0191395; -.
DR   PaxDb; Q6TMJ1; -.
DR   EnsemblProtists; EAL63276; EAL63276; DDB_G0288359.
DR   GeneID; 8626563; -.
DR   KEGG; ddi:DDB_G0288359; -.
DR   dictyBase; DDB_G0288359; dcd3A.
DR   eggNOG; KOG2329; Eukaryota.
DR   HOGENOM; CLU_063293_3_0_1; -.
DR   InParanoid; Q6TMJ1; -.
DR   OMA; IMFEPLR; -.
DR   PhylomeDB; Q6TMJ1; -.
DR   Reactome; R-DDI-1660661; Sphingolipid de novo biosynthesis.
DR   PRO; PR:Q6TMJ1; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0070774; F:phytoceramidase activity; IBA:GO_Central.
DR   GO; GO:0006672; P:ceramide metabolic process; IEA:InterPro.
DR   InterPro; IPR008901; ACER.
DR   PANTHER; PTHR46187; PTHR46187; 1.
DR   Pfam; PF05875; Ceramidase; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Hydrolase; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..288
FT                   /note="Putative alkaline ceramidase dcd3A"
FT                   /id="PRO_0000390656"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        78..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        105..125
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        206..226
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        240..260
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        23
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   288 AA;  33143 MW;  F468EB1469436EE0 CRC64;
     MDYIENQGAY GTPTASIDWC ELNYTYSPYI AEFYNTFSSL IISLFGIYGI WIMMPNFGTG
     VEKEHIKILK QLDVRNKVIL SYISLIVVGV GSAFYHATLL YQNQLFDELP MIYTALIMLY
     IMVTVGEEKT KKGFKGGVLG NSLLRHLLPY LLIAYGLFVT ITILVIQDQP KILQVSFGAL
     VFYVVFHSIY LINKKKPDGM PSNPDSYLYK YAFVSMLVGF TCWVVERYFC KNGKTFGFQL
     HAFWHFFTGM STYVWTQFLI CKLLEAKHYC VGIKHTLGLP RIHAIKRF