ID   DCD_DRYCA               Reviewed;         685 AA.
AC   B3Y522;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Dammaradiene synthase;
DE            EC=5.4.99.37;
GN   Name=DCD;
OS   Dryopteris crassirhizoma (Thick stemmed wood fern).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Polypodiopsida; Polypodiidae; Polypodiales; Polypodiineae; Dryopteridaceae;
OC   Dryopteridoideae; Dryopteris.
OX   NCBI_TaxID=97234;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=18790509; DOI=10.1016/j.phytochem.2008.07.017;
RA   Shinozaki J., Shibuya M., Masuda K., Ebizuka Y.;
RT   "Dammaradiene synthase, a squalene cyclase, from Dryopteris crassirhizoma
RT   Nakai.";
RL   Phytochemistry 69:2559-2564(2008).
CC   -!- FUNCTION: Squalene cyclase producing the tetracyclic triterpene
CC       dammaradiene. {ECO:0000269|PubMed:18790509}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=squalene = dammara-20,24-diene; Xref=Rhea:RHEA:31023,
CC         ChEBI:CHEBI:15440, ChEBI:CHEBI:62447; EC=5.4.99.37;
CC         Evidence={ECO:0000269|PubMed:18790509};
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB429303; BAG68223.1; -; mRNA.
DR   KEGG; ag:BAG68223; -.
DR   BioCyc; MetaCyc:MON-14418; -.
DR   BRENDA; 5.4.99.37; 12245.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016866; F:intramolecular transferase activity; IEA:InterPro.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR006400; Hopene-cyclase.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01507; hopene_cyclase; 1.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..685
FT                   /note="Dammaradiene synthase"
FT                   /id="PRO_0000413961"
FT   REPEAT          82..123
FT                   /note="PFTB 1"
FT   REPEAT          265..308
FT                   /note="PFTB 2"
FT   REPEAT          424..465
FT                   /note="PFTB 3"
FT   REPEAT          621..672
FT                   /note="PFTB 4"
FT   ACT_SITE        400
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   685 AA;  77463 MW;  E09CEB4918D76056 CRC64;
     MLPYNQDFYN EDEALKDDHC EGAGNVSNPP TLDEAIKRSQ DFLLSQQYPE GYWWAELEGN
     PTITSHTVIL YKILGIEDEY PMDKMEKYLR RMQCIHGGWE LFYGDGGQLS VTIESYVALR
     LLNVPPTDPA LKKALKFIID KGGVXKSRMF TKICLALLGC FDWRGIPSLP PWVMLLPGWF
     LSSIYETACW ARGCVVPLIV VFDKKPVFKV SPEVSFDELY AEGREHACKT LPFCGDWTSH
     FFIAVDRVFK MMERLGVVPF QQWGIREAEK WLLERQEDTG DFLGVYPPMF YSVVCMKTLG
     YEVTDPVVRR ALLSFKKFSI ERADECSVQS SLSPVWDTAL VVRSLVESGL PPDHPALQRA
     GEWLLQKQIT KHGDWSFKNQ SGVAGGWAFQ FFNRWYPDLD DSAVVVMALD CLKLPNEDVK
     NGAITRCLKW ISSMQCKGGG WAAFDKDNHQ HWINSTPFSD LKAMVDPSTT DISARVLEMV
     GRLKLHGTSF DEAHFLPPES IARGLVYLRR EQENEGCWFG RWGVNYIYGT CGALVALSLV
     APMTHEEEIA RGARWLVQVQ NMHGKKINGP QDGGWGETCF SYNDPALKGQ GDVSTASQTA
     WALQGLLAAG DALGKYEVES IGHGVQYLLS TQRKDGSWHE SQFTGGGFPI HFYLRYHFYA
     QHFTLSSLAR YRTRLQASKI KPPIP