ID   DCHM_BPT2               Reviewed;         246 AA.
AC   P18029;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   23-FEB-2022, entry version 87.
DE   RecName: Full=Deoxycytidylate 5-hydroxymethyltransferase;
DE            Short=Deoxycytidylate hydroxymethylase;
DE            EC=2.1.2.8;
DE   AltName: Full=dCMP hydroxymethylase;
GN   Name=42;
OS   Enterobacteria phage T2 (Bacteriophage T2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10664;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3350013; DOI=10.1111/j.1432-1033.1988.tb13925.x;
RA   Lamm N., Wang Y., Mathews C.K., Rueger W.;
RT   "Deoxycytidylate hydroxymethylase gene of bacteriophage T4. Nucleotide
RT   sequence determination and over-expression of the gene.";
RL   Eur. J. Biochem. 172:553-563(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 218-246.
RX   PubMed=8464751; DOI=10.1093/nar/21.6.1500;
RA   Winkler M., Rueger W.;
RT   "Cloning and sequencing of the genes of beta-glucosyl-HMC-alpha-glucosyl-
RT   transferases of bacteriophages T2 and T6.";
RL   Nucleic Acids Res. 21:1500-1500(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dCMP + H2O =
CC         (6S)-5,6,7,8-tetrahydrofolate + 5-hydroxymethyl-dCMP;
CC         Xref=Rhea:RHEA:11280, ChEBI:CHEBI:15377, ChEBI:CHEBI:15636,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57566, ChEBI:CHEBI:57962; EC=2.1.2.8;
CC   -!- SIMILARITY: Belongs to the thymidylate synthase family. {ECO:0000305}.
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DR   EMBL; X68724; CAA48663.1; -; Genomic_DNA.
DR   PIR; A34273; SZBPT2.
DR   SMR; P18029; -.
DR   GO; GO:0047153; F:deoxycytidylate 5-hydroxymethyltransferase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.30.572.10; -; 1.
DR   InterPro; IPR014619; Deoxycytidylate_hydroxyMease.
DR   InterPro; IPR045097; Thymidate_synth/dCMP_Mease.
DR   InterPro; IPR023451; Thymidate_synth/dCMP_Mease_dom.
DR   InterPro; IPR036926; Thymidate_synth/dCMP_Mease_sf.
DR   PANTHER; PTHR11548; PTHR11548; 1.
DR   Pfam; PF00303; Thymidylat_synt; 1.
DR   PIRSF; PIRSF036750; dCMP_HMase; 1.
DR   SUPFAM; SSF55831; SSF55831; 1.
PE   3: Inferred from homology;
KW   Transferase.
FT   CHAIN           1..246
FT                   /note="Deoxycytidylate 5-hydroxymethyltransferase"
FT                   /id="PRO_0000141067"
FT   ACT_SITE        148
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   246 AA;  28583 MW;  3EAB0DCE35351E8E CRC64;
     MISDSMTVEE IRLHLGLALK EKDFVVDKTG VKTIEIIGAS FVADEPFIFG ALNDEYIQRE
     LEWYKSKSLF VKDIPGETPK IWQQVASSKG EINSNYGWAI WSEDNYAQYD MCLAELGQNP
     DSRRGIMIYT RPSMQFDYNK DGMSDFMCTN TVQYLIRDKK INAVVNMRSN DVVFGFRNDY
     AWQKYVLDKL VSDLNAGDST RQYKAGSIIW NVGSLHVYSR HFYLVDHWWN TGETHIVKKD
     YNGEWK