DCLK3_HUMAN
ID DCLK3_HUMAN Reviewed; 648 AA.
AC Q9C098;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Serine/threonine-protein kinase DCLK3;
DE EC=2.7.11.1;
DE AltName: Full=Doublecortin domain-containing protein 3C;
DE AltName: Full=Doublecortin-like and CAM kinase-like 3;
DE AltName: Full=Doublecortin-like kinase 3;
GN Name=DCLK3; Synonyms=DCAMKL3, DCDC3C, KIAA1765;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-105.
RC TISSUE=Brain;
RX PubMed=11214970; DOI=10.1093/dnares/7.6.347;
RA Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XIX. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 7:347-355(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 41-648.
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP VARIANTS [LARGE SCALE ANALYSIS] GLN-24; LEU-108; LYS-422; ASN-472; CYS-554;
RP ARG-570; ALA-596 AND ASP-633.
RX PubMed=17344846; DOI=10.1038/nature05610;
RA Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G.,
RA Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S.,
RA Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.,
RA Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K.,
RA Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D.,
RA Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R.,
RA Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A.,
RA Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F.,
RA Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F.,
RA Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G.,
RA Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R.,
RA Futreal P.A., Stratton M.R.;
RT "Patterns of somatic mutation in human cancer genomes.";
RL Nature 446:153-158(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr
CC protein kinase family. CaMK subfamily. {ECO:0000305}.
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DR EMBL; DA523113; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AB051552; BAB21856.1; -; mRNA.
DR CCDS; CCDS43064.1; -.
DR RefSeq; NP_208382.1; NM_033403.1.
DR RefSeq; XP_011532469.1; XM_011534167.2.
DR AlphaFoldDB; Q9C098; -.
DR SMR; Q9C098; -.
DR BioGRID; 124530; 2.
DR IntAct; Q9C098; 8.
DR STRING; 9606.ENSP00000394484; -.
DR BindingDB; Q9C098; -.
DR ChEMBL; CHEMBL6123; -.
DR DrugBank; DB12010; Fostamatinib.
DR DrugCentral; Q9C098; -.
DR iPTMnet; Q9C098; -.
DR PhosphoSitePlus; Q9C098; -.
DR BioMuta; DCLK3; -.
DR DMDM; 115502142; -.
DR jPOST; Q9C098; -.
DR MassIVE; Q9C098; -.
DR MaxQB; Q9C098; -.
DR PaxDb; Q9C098; -.
DR PeptideAtlas; Q9C098; -.
DR PRIDE; Q9C098; -.
DR Antibodypedia; 28151; 103 antibodies from 30 providers.
DR DNASU; 85443; -.
DR Ensembl; ENST00000416516.2; ENSP00000394484.2; ENSG00000163673.8.
DR GeneID; 85443; -.
DR KEGG; hsa:85443; -.
DR UCSC; uc003cgi.2; human.
DR CTD; 85443; -.
DR DisGeNET; 85443; -.
DR GeneCards; DCLK3; -.
DR HGNC; HGNC:19005; DCLK3.
DR HPA; ENSG00000163673; Tissue enhanced (brain, testis).
DR MIM; 613167; gene.
DR neXtProt; NX_Q9C098; -.
DR OpenTargets; ENSG00000163673; -.
DR PharmGKB; PA162383403; -.
DR VEuPathDB; HostDB:ENSG00000163673; -.
DR eggNOG; KOG0032; Eukaryota.
DR GeneTree; ENSGT00940000159476; -.
DR HOGENOM; CLU_000288_94_2_1; -.
DR InParanoid; Q9C098; -.
DR OMA; ELDMGKC; -.
DR OrthoDB; 330091at2759; -.
DR PhylomeDB; Q9C098; -.
DR TreeFam; TF318770; -.
DR PathwayCommons; Q9C098; -.
DR SignaLink; Q9C098; -.
DR BioGRID-ORCS; 85443; 13 hits in 1102 CRISPR screens.
DR GenomeRNAi; 85443; -.
DR Pharos; Q9C098; Tchem.
DR PRO; PR:Q9C098; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q9C098; protein.
DR Bgee; ENSG00000163673; Expressed in sural nerve and 96 other tissues.
DR ExpressionAtlas; Q9C098; baseline and differential.
DR Genevisible; Q9C098; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004672; F:protein kinase activity; IBA:GO_Central.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0018105; P:peptidyl-serine phosphorylation; IBA:GO_Central.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; Kinase; Nucleotide-binding; Nucleus;
KW Reference proteome; Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..648
FT /note="Serine/threonine-protein kinase DCLK3"
FT /id="PRO_0000252254"
FT DOMAIN 356..613
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 86..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 150..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 628..648
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 169..183
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 197..340
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 477
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 362..370
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 385
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT VARIANT 24
FT /note="R -> Q (in dbSNP:rs56070233)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_040444"
FT VARIANT 108
FT /note="P -> L (in a breast infiltrating ductal carcinoma
FT sample; somatic mutation)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_040445"
FT VARIANT 422
FT /note="E -> K (in a colorectal adenocarcinoma sample;
FT somatic mutation; dbSNP:rs373605259)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_040446"
FT VARIANT 472
FT /note="S -> N (in a lung large cell carcinoma sample;
FT somatic mutation)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_040447"
FT VARIANT 554
FT /note="R -> C (in a lung squamous cell carcinoma sample;
FT somatic mutation; dbSNP:rs374711533)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_040448"
FT VARIANT 570
FT /note="G -> R (in a renal clear cell carcinoma sample;
FT somatic mutation)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_040449"
FT VARIANT 596
FT /note="V -> A (in a colorectal adenocarcinoma sample;
FT somatic mutation)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_040450"
FT VARIANT 633
FT /note="E -> D (in dbSNP:rs35704209)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_040451"
SQ SEQUENCE 648 AA; 73814 MW; 09E37E51920F2A21 CRC64;
MGKEPLTLKS IQVAVEELYP NKARALTLAQ HSRAPSPRLR SRLFSKALKG DHRCGETETP
KSCSEVAGCK AAMRHQGKIP EELSLDDRAR TQKKWGRGKW EPEPSSKPPR EATLEERHAR
GEKHLGVEIE KTSGEIIRCE KCKRERELQQ SLERERLSLG TSELDMGKGP MYDVEKLVRT
RSCRRSPEAN PASGEEGWKG DSHRSSPRNP TQELRRPSKS MDKKEDRGPE DQESHAQGAA
KAKKDLVEVL PVTEEGLREV KKDTRPMSRS KHGGWLLREH QAGFEKLRRT RGEEKEAEKE
KKPCMSGGRR MTLRDDQPAK LEKEPKTRPE ENKPERPSGR KPRPMGIIAA NVEKHYETGR
VIGDGNFAVV KECRHRETRQ AYAMKIIDKS RLKGKEDMVD SEILIIQSLS HPNIVKLHEV
YETDMEIYLI LEYVQGGDLF DAIIESVKFP EPDAALMIMD LCKALVHMHD KSIVHRDLKP
ENLLVQRNED KSTTLKLADF GLAKHVVRPI FTVCGTPTYV APEILSEKGY GLEVDMWAAG
VILYILLCGF PPFRSPERDQ DELFNIIQLG HFEFLPPYWD NISDAAKDLV SRLLVVDPKK
RYTAHQVLQH PWIETAGKTN TVKRQKQVSP SSEGHFRSQH KRVVEQVS
