ID   DCOA_KLEPN              Reviewed;         596 AA.
AC   P13187;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Oxaloacetate decarboxylase alpha chain;
DE            EC=7.2.4.2;
GN   Name=oadA;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2454915; DOI=10.1016/s0021-9258(19)81564-8;
RA   Schwarz E., Oesterhelt D., Reinke H., Beyreuther K., Dimroth P.;
RT   "The sodium ion translocating oxalacetate decarboxylase of Klebsiella
RT   pneumoniae. Sequence of the biotin-containing alpha-subunit and
RT   relationship to other biotin-containing enzymes.";
RL   J. Biol. Chem. 263:9640-9645(1988).
CC   -!- FUNCTION: Catalyzes the decarboxylation of oxaloacetate coupled to
CC       Na(+) translocation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + 2 Na(+)(in) + oxaloacetate = CO2 + 2 Na(+)(out) +
CC         pyruvate; Xref=Rhea:RHEA:57724, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16526, ChEBI:CHEBI:29101; EC=7.2.4.2;
CC   -!- COFACTOR:
CC       Name=biotin; Xref=ChEBI:CHEBI:57586;
CC   -!- SUBUNIT: Composed of three chains (alpha, beta, and gamma).
CC       {ECO:0000250}.
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DR   EMBL; J03885; AAA25120.1; -; Genomic_DNA.
DR   PIR; A28088; A28088.
DR   RefSeq; WP_004222624.1; NZ_WVHM01000014.1.
DR   PDB; 6ROJ; EM; 2.90 A; A=508-593.
DR   PDBsum; 6ROJ; -.
DR   AlphaFoldDB; P13187; -.
DR   SMR; P13187; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0015451; F:decarboxylation-driven active transmembrane transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0008948; F:oxaloacetate decarboxylase activity; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR001882; Biotin_BS.
DR   InterPro; IPR000089; Biotin_lipoyl.
DR   InterPro; IPR003379; Carboxylase_cons_dom.
DR   InterPro; IPR005776; OadA.
DR   InterPro; IPR000891; PYR_CT.
DR   InterPro; IPR011053; Single_hybrid_motif.
DR   Pfam; PF00364; Biotin_lipoyl; 1.
DR   Pfam; PF00682; HMGL-like; 1.
DR   Pfam; PF02436; PYC_OADA; 1.
DR   SUPFAM; SSF51230; SSF51230; 1.
DR   TIGRFAMs; TIGR01108; oadA; 1.
DR   PROSITE; PS00188; BIOTIN; 1.
DR   PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Biotin; Ion transport; Sodium; Sodium transport; Translocase;
KW   Transport.
FT   INIT_MET        1
FT                   /note="Removed"
FT   CHAIN           2..596
FT                   /note="Oxaloacetate decarboxylase alpha chain"
FT                   /id="PRO_0000146833"
FT   DOMAIN          3..263
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01151"
FT   DOMAIN          523..596
FT                   /note="Biotinyl-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01066"
FT   REGION          503..526
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         562
FT                   /note="N6-biotinyllysine"
FT                   /evidence="ECO:0000250, ECO:0000255|PROSITE-
FT                   ProRule:PRU01066"
SQ   SEQUENCE   596 AA;  63534 MW;  284928FC45397054 CRC64;
     MTVAITDVVL RDAHQSLFAT RLRLDDMLPV AAQLDDVGYR SLECWGGATF DACIRFLGED
     PWVRLRELKK AMPKTPLQML LRGQNLLGYR HYADDVVERF VERAVKNGMD VFRVFDAMND
     PRNMQAALQA VRRHGAHAQG TLSYTTSPAH TLQTWLDLTE QLLETGVDSV AIKDMSGILT
     PHAAFELVSE IKKRYDVTLH LHCHATTGMA EMALLKAIEA GVDGVDTAIS SMSATYGHPA
     TEALVATLAG TPYDTGLDIH KLESIAAYFR EVRKKYHAFE GQLKGTDSRI LVAQVPGGML
     TNLEGQLKQQ SAAHRLDEVL AEIPRVREDL GFIPLVTPTS QIVGTQAVLN VLTGERYKTI
     AKETAGILKG EYGRTPAPVN AALQARVLDG ADPVTCRPAD LLKPELAQLE ADVRRQAQEK
     GITLAENAID DVLTVALFPQ PGLKFLENRH NPAAFEPVPQ AEAAQPVAKA EKPAASGVYT
     VEVEGKAFVV KVSDGGDVSQ LTAAAPAPAP APAPASAPAA AAPAGAGTPV TAPLAGTIWK
     VLASEGQTVA AGEVLLILEA MKMETEIRAA QAGTVRGIAV KAGDAVAVGD TLMTLA