ID   DCTA2_CUPMC             Reviewed;         452 AA.
AC   Q1LFB1;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=C4-dicarboxylate transport protein 2 {ECO:0000255|HAMAP-Rule:MF_01300};
GN   Name=dctA2 {ECO:0000255|HAMAP-Rule:MF_01300}; OrderedLocusNames=Rmet_4299;
OS   Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS   CH34) (Ralstonia metallidurans).
OG   Plasmid megaplasmid CH34.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=266264;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RX   PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA   Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA   Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA   Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT   "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT   master survivalist in harsh and anthropogenic environments.";
RL   PLoS ONE 5:E10433-E10433(2010).
CC   -!- FUNCTION: Responsible for the transport of dicarboxylates such as
CC       succinate, fumarate, and malate from the periplasm across the membrane.
CC       {ECO:0000255|HAMAP-Rule:MF_01300}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01300}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01300}.
CC   -!- SIMILARITY: Belongs to the dicarboxylate/amino acid:cation symporter
CC       (DAACS) (TC 2.A.23) family. {ECO:0000255|HAMAP-Rule:MF_01300}.
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DR   EMBL; CP000353; ABF11165.1; -; Genomic_DNA.
DR   RefSeq; WP_011518792.1; NC_007974.2.
DR   AlphaFoldDB; Q1LFB1; -.
DR   SMR; Q1LFB1; -.
DR   STRING; 266264.Rmet_4299; -.
DR   EnsemblBacteria; ABF11165; ABF11165; Rmet_4299.
DR   KEGG; rme:Rmet_4299; -.
DR   eggNOG; COG1301; Bacteria.
DR   HOGENOM; CLU_019375_7_0_4; -.
DR   OMA; VAMKKPQ; -.
DR   OrthoDB; 781228at2; -.
DR   Proteomes; UP000002429; Plasmid megaplasmid CH34.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006835; P:dicarboxylic acid transport; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.3860.10; -; 1.
DR   HAMAP; MF_01300; C4_dicarb_transport; 1.
DR   InterPro; IPR023954; C4_dicarb_transport.
DR   InterPro; IPR001991; Na-dicarboxylate_symporter.
DR   InterPro; IPR018107; Na-dicarboxylate_symporter_CS.
DR   InterPro; IPR036458; Na:dicarbo_symporter_sf.
DR   PANTHER; PTHR42865; PTHR42865; 1.
DR   Pfam; PF00375; SDF; 1.
DR   SUPFAM; SSF118215; SSF118215; 1.
DR   PROSITE; PS00713; NA_DICARBOXYL_SYMP_1; 1.
DR   PROSITE; PS00714; NA_DICARBOXYL_SYMP_2; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Plasmid; Reference proteome;
KW   Symport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..452
FT                   /note="C4-dicarboxylate transport protein 2"
FT                   /id="PRO_0000321996"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        49..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        189..209
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        227..247
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        294..314
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        336..356
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   REGION          417..452
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   452 AA;  48450 MW;  D69D301450987354 CRC64;
     MEKVDNRKPL YRSLYFQVIV AIISGVVLGH YYPTAGEAMK PLGDGFIKLI KMIIAPIIFC
     TVVVGIAGME DMKKVGKTGG LALLYFEVVS SIALLLGLLI INIVKPGAGM NVDPSTLDTK
     GIAAYTGPGK MQGTVDFLMN VIPNTVVDAF AKGEILQVLL FAVMFGFALH KFGGRGTLVF
     DFIEKFSHVL FTIVGYIMKV APLGAFGAMA FTIGKYGVSS LLQLGQLMAT FYATCLVFIF
     VVLGTIARLH GFSIWKFIKY IKEELLIVLG TSSSESVLPR MMAKLENLGV RKSVVGLVIP
     TGYSFNLDGT SIYLTMAAVF IAQATNTDMN LTQQLTLLAV LLLTSKGAAG VTGSGFIVLA
     ATLSAVGHVP VAGLALILGI DRFMSEARAL TNLIGNGVAT VVVAKWTGDL DLERMRRRLD
     DETDSEADEP EMVLDQTATH MPAVDPAVTR RG