ID   DCTA_POLAQ              Reviewed;         442 AA.
AC   A4T0I1;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=C4-dicarboxylate transport protein {ECO:0000255|HAMAP-Rule:MF_01300};
GN   Name=dctA {ECO:0000255|HAMAP-Rule:MF_01300}; OrderedLocusNames=Pnuc_2034;
OS   Polynucleobacter asymbioticus (strain DSM 18221 / CIP 109841 /
OS   QLW-P1DMWA-1) (Polynucleobacter necessarius subsp. asymbioticus).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Polynucleobacter.
OX   NCBI_TaxID=312153;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 18221 / CIP 109841 / QLW-P1DMWA-1;
RX   PubMed=22675600; DOI=10.4056/sigs.2395367;
RA   Meincke L., Copeland A., Lapidus A., Lucas S., Berry K.W., Del Rio T.G.,
RA   Hammon N., Dalin E., Tice H., Pitluck S., Richardson P., Bruce D.,
RA   Goodwin L., Han C., Tapia R., Detter J.C., Schmutz J., Brettin T.,
RA   Larimer F., Land M., Hauser L., Kyrpides N.C., Ivanova N., Goker M.,
RA   Woyke T., Wu Q.L., Pockl M., Hahn M.W., Klenk H.P.;
RT   "Complete genome sequence of Polynucleobacter necessarius subsp.
RT   asymbioticus type strain (QLW-P1DMWA-1(T)).";
RL   Stand. Genomic Sci. 6:74-83(2012).
CC   -!- FUNCTION: Responsible for the transport of dicarboxylates such as
CC       succinate, fumarate, and malate across the membrane.
CC       {ECO:0000255|HAMAP-Rule:MF_01300}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01300};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01300}.
CC   -!- SIMILARITY: Belongs to the dicarboxylate/amino acid:cation symporter
CC       (DAACS) (TC 2.A.23) family. {ECO:0000255|HAMAP-Rule:MF_01300}.
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DR   EMBL; CP000655; ABP35245.1; -; Genomic_DNA.
DR   RefSeq; WP_011903868.1; NC_009379.1.
DR   AlphaFoldDB; A4T0I1; -.
DR   SMR; A4T0I1; -.
DR   STRING; 312153.Pnuc_2034; -.
DR   EnsemblBacteria; ABP35245; ABP35245; Pnuc_2034.
DR   GeneID; 31482424; -.
DR   KEGG; pnu:Pnuc_2034; -.
DR   eggNOG; COG1301; Bacteria.
DR   HOGENOM; CLU_019375_7_0_4; -.
DR   OMA; QATNTPM; -.
DR   Proteomes; UP000000231; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006835; P:dicarboxylic acid transport; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.3860.10; -; 1.
DR   HAMAP; MF_01300; C4_dicarb_transport; 1.
DR   InterPro; IPR023954; C4_dicarb_transport.
DR   InterPro; IPR001991; Na-dicarboxylate_symporter.
DR   InterPro; IPR018107; Na-dicarboxylate_symporter_CS.
DR   InterPro; IPR036458; Na:dicarbo_symporter_sf.
DR   PANTHER; PTHR42865; PTHR42865; 1.
DR   Pfam; PF00375; SDF; 1.
DR   SUPFAM; SSF118215; SSF118215; 1.
DR   PROSITE; PS00714; NA_DICARBOXYL_SYMP_2; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Symport; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..442
FT                   /note="C4-dicarboxylate transport protein"
FT                   /id="PRO_1000085902"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        49..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        193..213
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        227..247
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        312..332
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        336..356
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01300"
SQ   SEQUENCE   442 AA;  47322 MW;  163930F8FE386D81 CRC64;
     MTATLKKPPI YKVLYFQVLT AVVIGVLLGH FYPSLGVDMK PFGDAFIKGI KMLIAPIIFC
     TVVLGIAGME DMKKVGKTGG LALLYFEIVS TIALMVGLVV VNVLQPGAGM NIDPASLDTK
     GIAAYTGPGK MGTTTEFLLN IIPSSAVDAF AKGEILQVLF IAILFGFALH KFGGRGTMVF
     DLIEKTSHVL FDMIAIIMKF APIGAFGAMA FTIGKYGIGS LFSLGKLMGS FYLTCLLFVF
     IVLGIIARLN GFNIFKFVRY IKEELLIVLG TSSSESVLPR MMEKMELLGA KKACVGLVIP
     TGYSFNLDGT SIYLTMAAVF IAQATNTPMT LLQEVTLLAV LLLTSKGAAG VTGSGFIVLA
     ATLSAVGDVP VAGLAIILGI DRFMSEARAL TNLVGNGVAT IVVAKWTGEL DQKQLTRALN
     RDDWIEAQEP EMILDHQQDK MR