DCTA_SINFN
ID DCTA_SINFN Reviewed; 497 AA.
AC P31601; Q53214;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 05-MAR-2002, sequence version 4.
DT 25-MAY-2022, entry version 141.
DE RecName: Full=C4-dicarboxylate transport protein;
GN Name=dctA1; OrderedLocusNames=NGR_a01180; ORFNames=y4vF;
OS Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG Plasmid sym pNGR234a.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=394;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1617199; DOI=10.1094/mpmi-5-179;
RA van Slooten J.C., Bhuvanasvari T.V., Bardin S., Stanley J.;
RT "Two C4-dicarboxylate transport systems in Rhizobium sp. NGR234: rhizobial
RT dicarboxylate transport is essential for nitrogen fixation in tropical
RT legume symbioses.";
RL Mol. Plant Microbe Interact. 5:179-186(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8796346; DOI=10.1101/gr.6.7.590;
RA Freiberg C., Perret X., Broughton W.J., Rosenthal A.;
RT "Sequencing the 500-kb GC-rich symbiotic replicon of Rhizobium sp. NGR234
RT using dye terminators and a thermostable 'sequenase': a beginning.";
RL Genome Res. 6:590-600(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=9163424; DOI=10.1038/387394a0;
RA Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA Perret X.;
RT "Molecular basis of symbiosis between Rhizobium and legumes.";
RL Nature 387:394-401(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=19376903; DOI=10.1128/aem.00515-09;
RA Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT systems.";
RL Appl. Environ. Microbiol. 75:4035-4045(2009).
CC -!- FUNCTION: Responsible for the transport of dicarboxylates such as
CC succinate, fumarate, and malate from the periplasm across the inner
CC membrane. This transport system plays an essential role in the energy
CC supply of tropical rhizobium-legume symbionts.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- INDUCTION: By succinate, fumarate, and malate. Expression depends on
CC the RpoN sigma factor.
CC -!- SIMILARITY: Belongs to the dicarboxylate/amino acid:cation symporter
CC (DAACS) (TC 2.A.23) family. {ECO:0000305}.
CC -!- CAUTION: There are two genes for DctA in NGR234; one on the sym
CC plasmid, the other on the chromosome. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB22400.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAB91894.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA92421.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; S38912; AAB22400.1; ALT_INIT; Genomic_DNA.
DR EMBL; S38912; AAB22401.1; -; Genomic_DNA.
DR EMBL; Z68203; CAA92421.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00090; AAB91894.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_444107.1; NC_000914.2.
DR AlphaFoldDB; P31601; -.
DR SMR; P31601; -.
DR STRING; 394.NGR_a01180; -.
DR EnsemblBacteria; AAB91894; AAB91894; NGR_a01180.
DR KEGG; rhi:NGR_a01180; -.
DR PATRIC; fig|394.7.peg.102; -.
DR eggNOG; COG1301; Bacteria.
DR HOGENOM; CLU_019375_7_0_5; -.
DR OMA; VAMKKPQ; -.
DR OrthoDB; 781228at2; -.
DR Proteomes; UP000001054; Plasmid sym pNGR234a.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006835; P:dicarboxylic acid transport; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.3860.10; -; 1.
DR HAMAP; MF_01300; C4_dicarb_transport; 1.
DR InterPro; IPR023954; C4_dicarb_transport.
DR InterPro; IPR001991; Na-dicarboxylate_symporter.
DR InterPro; IPR018107; Na-dicarboxylate_symporter_CS.
DR InterPro; IPR036458; Na:dicarbo_symporter_sf.
DR PANTHER; PTHR42865; PTHR42865; 1.
DR Pfam; PF00375; SDF; 1.
DR SUPFAM; SSF118215; SSF118215; 1.
DR PROSITE; PS00713; NA_DICARBOXYL_SYMP_1; 1.
DR PROSITE; PS00714; NA_DICARBOXYL_SYMP_2; 1.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Membrane; Plasmid; Reference proteome;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..497
FT /note="C4-dicarboxylate transport protein"
FT /id="PRO_0000202110"
FT TRANSMEM 27..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 466..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 101
FT /note="T -> A (in Ref. 1; AAB22400/AAB22401)"
FT /evidence="ECO:0000305"
FT CONFLICT 337
FT /note="A -> G (in Ref. 1; AAB22400/AAB22401)"
FT /evidence="ECO:0000305"
FT CONFLICT 437..497
FT /note="PVETMSGLSSQRSDTVELGQKVLFGATNSADRTLAGRPGGRDSRRIAPDHSA
FT QVFGGPLSL -> AGGDHVGVVKPAE (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 497 AA; 52129 MW; 49E960D8845B2824 CRC64;
MHQVEEIILI VENLAEVRGK TPHYRHLYVQ VLAAIAVGIL LGYFYPDVGS KMKPLGDAFI
MLVKMIIAPV IFLTVATGIA GMTDLAKVGR VAGKAMIYFL TFSTLALLVG LVVANVVQPG
AGMHIDPASL DAKAIATYAE KAHEQSVTGF LMNIIPTTLV GAFAEGDILQ VLFISVLFGI
SLAIVGKKAE AVVDFLHALT LPIFRLVAIL MKAAPIGAFG AMAFTIGKYG VASIANLAML
IGTFYLTSFL FVFMVLGAVA RYNGFSIVAL IRYIKEELLL VLGTSSSEAA LPGLMNKMEK
AGCKRSVVGL VIPTGYSFNL DGTNIYMTLA ALFIAQATDT PISYGDQILL LLIAMLSSKG
AAGITGAGFI TLAATLSAVP SVPVAGMALI LGIDRFMSEC RAITNIIGNA VATIVVAKWE
GELAPAQLAT TLAGKAPVET MSGLSSQRSD TVELGQKVLF GATNSADRTL AGRPGGRDSR
RIAPDHSAQV FGGPLSL
