DCTD_CAEEL
ID DCTD_CAEEL Reviewed; 197 AA.
AC P30648;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Probable deoxycytidylate deaminase;
DE EC=3.5.4.12;
DE AltName: Full=dCMP deaminase;
GN ORFNames=ZK643.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=1538779; DOI=10.1038/356037a0;
RA Sulston J., Du Z., Thomas K., Wilson R., Hillier L., Staden R.,
RA Halloran N., Green P., Thierry-Mieg J., Qiu L., Dear S., Coulson A.,
RA Craxton M., Durbin R., Berks M., Metzstein M., Hawkins T., Ainscough R.,
RA Waterston R.;
RT "The C. elegans genome sequencing project: a beginning.";
RL Nature 356:37-41(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Supplies the nucleotide substrate for thymidylate synthetase.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dCMP + H(+) + H2O = dUMP + NH4(+); Xref=Rhea:RHEA:22924,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:57566, ChEBI:CHEBI:246422; EC=3.5.4.12;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC family. {ECO:0000305}.
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DR EMBL; Z11126; CAA77473.1; -; Genomic_DNA.
DR PIR; S23240; S23240.
DR RefSeq; NP_498980.1; NM_066579.4.
DR AlphaFoldDB; P30648; -.
DR SMR; P30648; -.
DR STRING; 6239.ZK643.2; -.
DR ChEMBL; CHEMBL2448; -.
DR EPD; P30648; -.
DR PaxDb; P30648; -.
DR EnsemblMetazoa; ZK643.2.1; ZK643.2.1; WBGene00014034.
DR GeneID; 191373; -.
DR KEGG; cel:CELE_ZK643.2; -.
DR UCSC; ZK643.2; c. elegans.
DR CTD; 191373; -.
DR WormBase; ZK643.2; CE00441; WBGene00014034; -.
DR eggNOG; KOG3127; Eukaryota.
DR GeneTree; ENSGT00940000153676; -.
DR HOGENOM; CLU_047993_1_1_1; -.
DR InParanoid; P30648; -.
DR OMA; NTQVGCV; -.
DR OrthoDB; 1569973at2759; -.
DR PhylomeDB; P30648; -.
DR Reactome; R-CEL-499943; Interconversion of nucleotide di- and triphosphates.
DR PRO; PR:P30648; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00014034; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0004132; F:dCMP deaminase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006231; P:dTMP biosynthetic process; IBA:GO_Central.
DR GO; GO:0006226; P:dUMP biosynthetic process; IBA:GO_Central.
DR CDD; cd01286; deoxycytidylate_deaminase; 1.
DR InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd.
DR InterPro; IPR002125; CMP_dCMP_dom.
DR InterPro; IPR016193; Cytidine_deaminase-like.
DR InterPro; IPR015517; dCMP_deaminase-rel.
DR InterPro; IPR035105; Deoxycytidylate_deaminase_dom.
DR PANTHER; PTHR11086; PTHR11086; 1.
DR Pfam; PF00383; dCMP_cyt_deam_1; 1.
DR SUPFAM; SSF53927; SSF53927; 1.
DR PROSITE; PS00903; CYT_DCMP_DEAMINASES_1; 1.
DR PROSITE; PS51747; CYT_DCMP_DEAMINASES_2; 1.
PE 3: Inferred from homology;
KW Hydrolase; Metal-binding; Nucleotide biosynthesis; Reference proteome;
KW Zinc.
FT CHAIN 1..197
FT /note="Probable deoxycytidylate deaminase"
FT /id="PRO_0000171695"
FT DOMAIN 49..183
FT /note="CMP/dCMP-type deaminase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01083"
FT ACT_SITE 119
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 117
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 143
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 146
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
SQ SEQUENCE 197 AA; 22528 MW; 33B6A723AD3F9FE7 CRC64;
MVPPITSDEF CETSGCFGDS ANLHTTLERL KININSDAKK LVDTNGDLKK HQRFLRIAKV
TSLRSKDPNT QVGCVIVDKD NCIVSVGYNG FPIGVDDDVF RWDKEDPEDN KHLYVVHAEM
NAIINKRCTT LHDCTVYVTL FPCNKCAQML IQSRVKKVYF LENRDELAFR ASKKMLDHAR
LPYEQIVMPQ EAYVIEL