ID   DCTN2_DICDI             Reviewed;         430 AA.
AC   Q54N50;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Dynactin subunit 2;
GN   Name=dynB; Synonyms=dctn2; ORFNames=DDB_G0285503;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Modulates cytoplasmic dynein binding to an organelle, and
CC       plays a role in prometaphase chromosome alignment and spindle
CC       organization during mitosis. {ECO:0000250}.
CC   -!- SUBUNIT: Subunit of dynactin, a multiprotein complex associated with
CC       dynein. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}. Membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the dynactin subunit 2 family. {ECO:0000305}.
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DR   EMBL; AAFI02000079; EAL64589.1; -; Genomic_DNA.
DR   RefSeq; XP_638093.1; XM_633001.1.
DR   AlphaFoldDB; Q54N50; -.
DR   SMR; Q54N50; -.
DR   STRING; 44689.DDB0220488; -.
DR   PaxDb; Q54N50; -.
DR   EnsemblProtists; EAL64589; EAL64589; DDB_G0285503.
DR   GeneID; 8625141; -.
DR   KEGG; ddi:DDB_G0285503; -.
DR   dictyBase; DDB_G0285503; dynB.
DR   eggNOG; KOG3958; Eukaryota.
DR   HOGENOM; CLU_638458_0_0_1; -.
DR   InParanoid; Q54N50; -.
DR   OMA; RGYDMRG; -.
DR   PhylomeDB; Q54N50; -.
DR   Reactome; R-DDI-6807878; COPI-mediated anterograde transport.
DR   PRO; PR:Q54N50; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0005813; C:centrosome; TAS:dictyBase.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005869; C:dynactin complex; ISS:dictyBase.
DR   GO; GO:0030286; C:dynein complex; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0007017; P:microtubule-based process; IEA:InterPro.
DR   GO; GO:0000278; P:mitotic cell cycle; ISS:dictyBase.
DR   GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR   InterPro; IPR028133; Dynamitin.
DR   PANTHER; PTHR15346; PTHR15346; 1.
DR   Pfam; PF04912; Dynamitin; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Dynein; Membrane; Microtubule;
KW   Reference proteome.
FT   CHAIN           1..430
FT                   /note="Dynactin subunit 2"
FT                   /id="PRO_0000328015"
FT   REGION          1..51
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          201..228
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          241..319
FT                   /evidence="ECO:0000255"
FT   COILED          397..430
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        19..48
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   430 AA;  48867 MW;  9B455D1764E74BAB CRC64;
     MSEGNQPKRV LGLPDVDPSQ QDTFETPDPP ESISNLADES SELVNESIDR SSLKPAKSHL
     KFANRKFNND NEDFSDSIYK KSVPRTGGLL KNQTVFDILP SDESKVETPL QKFQRLQYEV
     MSFREEMQVI ADNGGEVEKD IKGVDLTHQL ADLQNQLSHL LENEKLQPIL DENKQVIHYS
     QIQNNSSKKL ISEIESFTQQ SLSSSTTEQN TQQPSNNNTT TNITSSSSIT PNHVTYELFY
     TGEQSKSQQL QRIQDLEKRL AKLETATGNK TTDSVPITQS ILEIKEKLSL LDTTKIDVLQ
     QKMKTVSKEM ESLKIQDETS TKALTTNEKK INDIFETMNK WDIVGQQVPA IINRLYTLRS
     LHEEGISFSN HVSNLEKQQN DITSLLISNS SLMNKMDDSF KSNLLTIKSN IQQLESRIET
     LQNRQQQQQQ