DCTN3_MOUSE
ID DCTN3_MOUSE Reviewed; 186 AA.
AC Q9Z0Y1; Q9CR43; Q9D039;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Dynactin subunit 3;
DE AltName: Full=Dynactin light chain p24;
GN Name=Dctn3 {ECO:0000312|MGI:MGI:1859251};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000312|EMBL:AAD16226.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9786090;
RX DOI=10.1002/(sici)1097-0169(1998)41:2<154::aid-cm6>3.0.co;2-e;
RA Pfister K.K., Benashski S.E., Dillman J.F. III, Patel-King R.S., King S.M.;
RT "Identification and molecular characterization of the p24 dynactin light
RT chain.";
RL Cell Motil. Cytoskeleton 41:154-167(1998).
RN [2] {ECO:0000312|EMBL:BAB27872.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAB27872.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:BAB27872.1}, and
RC Kidney {ECO:0000312|EMBL:BAB22307.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3] {ECO:0000312|EMBL:AAH61120.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis {ECO:0000312|EMBL:AAH61120.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Together with dynein may be involved in spindle assembly and
CC cytokinesis. {ECO:0000250|UniProtKB:O75935}.
CC -!- SUBUNIT: Subunit of dynactin, a multiprotein complex associated with
CC dynein.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton,
CC microtubule organizing center, centrosome {ECO:0000250}. Chromosome,
CC centromere, kinetochore {ECO:0000250}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250}. Cleavage furrow {ECO:0000250}. Midbody {ECO:0000250}.
CC Note=Localizes to punctate cytoplasmic structures and to the centrosome
CC during interphase, and to kinetochores and to spindle poles throughout
CC mitosis. Colocalizes with dynein to the cleavage furrow and to midbody
CC of dividing cells (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the dynactin subunit 3 family. {ECO:0000305}.
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DR EMBL; AF098508; AAD16226.1; -; mRNA.
DR EMBL; AK003255; BAB22671.1; -; mRNA.
DR EMBL; AK002720; BAB22307.1; -; mRNA.
DR EMBL; AK011840; BAB27872.1; -; mRNA.
DR EMBL; AK159344; BAE35006.1; -; mRNA.
DR EMBL; BC061120; AAH61120.1; -; mRNA.
DR CCDS; CCDS18069.1; -.
DR RefSeq; NP_001153037.1; NM_001159565.1.
DR RefSeq; NP_058586.3; NM_016890.4.
DR AlphaFoldDB; Q9Z0Y1; -.
DR SMR; Q9Z0Y1; -.
DR BioGRID; 207318; 84.
DR IntAct; Q9Z0Y1; 74.
DR MINT; Q9Z0Y1; -.
DR STRING; 10090.ENSMUSP00000030158; -.
DR iPTMnet; Q9Z0Y1; -.
DR PhosphoSitePlus; Q9Z0Y1; -.
DR EPD; Q9Z0Y1; -.
DR jPOST; Q9Z0Y1; -.
DR MaxQB; Q9Z0Y1; -.
DR PaxDb; Q9Z0Y1; -.
DR PeptideAtlas; Q9Z0Y1; -.
DR PRIDE; Q9Z0Y1; -.
DR ProteomicsDB; 279397; -.
DR Antibodypedia; 25511; 208 antibodies from 29 providers.
DR DNASU; 53598; -.
DR Ensembl; ENSMUST00000030158; ENSMUSP00000030158; ENSMUSG00000028447.
DR GeneID; 53598; -.
DR KEGG; mmu:53598; -.
DR UCSC; uc008sji.2; mouse.
DR CTD; 11258; -.
DR MGI; MGI:1859251; Dctn3.
DR VEuPathDB; HostDB:ENSMUSG00000028447; -.
DR eggNOG; ENOG502RYZ0; Eukaryota.
DR GeneTree; ENSGT00390000016210; -.
DR HOGENOM; CLU_121487_1_0_1; -.
DR InParanoid; Q9Z0Y1; -.
DR OMA; EYNKMTI; -.
DR OrthoDB; 1444664at2759; -.
DR PhylomeDB; Q9Z0Y1; -.
DR TreeFam; TF105248; -.
DR Reactome; R-MMU-2132295; MHC class II antigen presentation.
DR Reactome; R-MMU-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-MMU-3371497; HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand.
DR Reactome; R-MMU-380259; Loss of Nlp from mitotic centrosomes.
DR Reactome; R-MMU-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-MMU-380284; Loss of proteins required for interphase microtubule organization from the centrosome.
DR Reactome; R-MMU-380320; Recruitment of NuMA to mitotic centrosomes.
DR Reactome; R-MMU-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-MMU-6807878; COPI-mediated anterograde transport.
DR Reactome; R-MMU-6811436; COPI-independent Golgi-to-ER retrograde traffic.
DR Reactome; R-MMU-8854518; AURKA Activation by TPX2.
DR BioGRID-ORCS; 53598; 25 hits in 73 CRISPR screens.
DR ChiTaRS; Dctn3; mouse.
DR PRO; PR:Q9Z0Y1; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q9Z0Y1; protein.
DR Bgee; ENSMUSG00000028447; Expressed in saccule of membranous labyrinth and 257 other tissues.
DR ExpressionAtlas; Q9Z0Y1; baseline and differential.
DR Genevisible; Q9Z0Y1; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0032154; C:cleavage furrow; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005869; C:dynactin complex; IDA:MGI.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; ISO:MGI.
DR GO; GO:0005875; C:microtubule associated complex; IDA:MGI.
DR GO; GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0061640; P:cytoskeleton-dependent cytokinesis; ISS:UniProtKB.
DR GO; GO:0007017; P:microtubule-based process; IDA:MGI.
DR InterPro; IPR009991; DCTN3.
DR PANTHER; PTHR28360; PTHR28360; 1.
DR Pfam; PF07426; Dynactin_p22; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell cycle; Cell division; Centromere; Chromosome;
KW Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Mitosis;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:O75935"
FT CHAIN 2..186
FT /note="Dynactin subunit 3"
FT /id="PRO_0000225604"
FT COILED 46..66
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:O75935"
FT CONFLICT 3
FT /note="A -> G (in Ref. 2; BAB27872)"
FT /evidence="ECO:0000305"
FT CONFLICT 131
FT /note="Q -> E (in Ref. 1; AAD16226)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 186 AA; 20978 MW; 2630EA16F629D414 CRC64;
MAALTDVQRL QSRVEELERW VYGPGGTRGS RKVADGLVKV QVALGNIASK RERVKILYKK
IEDLIKYLDP EYIDRIAIPE ASKLQFILAE EQFILSQVAL LEQVNALVPV LDSASIKAVP
EHAARLQRLA QIHIQQQDQC VAITEESKAL LEGYNKTTML LSKQFVQWDE LLCQLEAAKQ
VKPAEE
