DCTN5_HUMAN
ID DCTN5_HUMAN Reviewed; 182 AA.
AC Q9BTE1; A8K9X8; H3BN51; H3BQA4;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Dynactin subunit 5;
DE AltName: Full=Dynactin subunit p25;
GN Name=DCTN5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Mammary gland, and Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA Myers R.M., Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Kidney, and Trophoblast;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Lymphoblast;
RX PubMed=14654843; DOI=10.1038/nature02166;
RA Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M.;
RT "Proteomic characterization of the human centrosome by protein correlation
RT profiling.";
RL Nature 426:570-574(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [9]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=23455152; DOI=10.1038/emboj.2013.30;
RA Yeh T.Y., Kowalska A.K., Scipioni B.R., Cheong F.K., Zheng M.,
RA Derewenda U., Derewenda Z.S., Schroer T.A.;
RT "Dynactin helps target Polo-like kinase 1 to kinetochores via its left-
RT handed beta-helical p27 subunit.";
RL EMBO J. 32:1023-1035(2013).
CC -!- SUBUNIT: Member of the pointed-end complex of the dynactin shoulder
CC complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10.
CC Within the complex DCTN6 forms a heterodimer with DCTN5.
CC {ECO:0000269|PubMed:23455152}.
CC -!- INTERACTION:
CC Q9BTE1; P63167: DYNLL1; NbExp=3; IntAct=EBI-747324, EBI-349105;
CC Q9BTE1; Q969F0: FATE1; NbExp=3; IntAct=EBI-747324, EBI-743099;
CC Q9BTE1; Q93062: RBPMS; NbExp=3; IntAct=EBI-747324, EBI-740322;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:23455152}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9BTE1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BTE1-2; Sequence=VSP_046023;
CC Name=3;
CC IsoId=Q9BTE1-3; Sequence=VSP_046697;
CC -!- SIMILARITY: Belongs to the dynactin subunits 5/6 family. Dynactin
CC subunit 5 subfamily. {ECO:0000305}.
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DR EMBL; AK027387; BAB55077.1; -; mRNA.
DR EMBL; AK292843; BAF85532.1; -; mRNA.
DR EMBL; AC008870; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471145; EAW55809.1; -; Genomic_DNA.
DR EMBL; CH471145; EAW55810.1; -; Genomic_DNA.
DR EMBL; BC004191; AAH04191.1; -; mRNA.
DR EMBL; CD388246; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS10615.1; -. [Q9BTE1-1]
DR CCDS; CCDS58435.1; -. [Q9BTE1-3]
DR CCDS; CCDS58436.1; -. [Q9BTE1-2]
DR RefSeq; NP_001185940.1; NM_001199011.1. [Q9BTE1-3]
DR RefSeq; NP_001186672.1; NM_001199743.1. [Q9BTE1-2]
DR RefSeq; NP_115875.1; NM_032486.3. [Q9BTE1-1]
DR PDB; 5NW4; EM; 8.70 A; h=1-182.
DR PDBsum; 5NW4; -.
DR AlphaFoldDB; Q9BTE1; -.
DR SMR; Q9BTE1; -.
DR BioGRID; 124110; 58.
DR IntAct; Q9BTE1; 30.
DR MINT; Q9BTE1; -.
DR STRING; 9606.ENSP00000300087; -.
DR iPTMnet; Q9BTE1; -.
DR PhosphoSitePlus; Q9BTE1; -.
DR BioMuta; DCTN5; -.
DR DMDM; 62900103; -.
DR EPD; Q9BTE1; -.
DR jPOST; Q9BTE1; -.
DR MassIVE; Q9BTE1; -.
DR MaxQB; Q9BTE1; -.
DR PaxDb; Q9BTE1; -.
DR PeptideAtlas; Q9BTE1; -.
DR PRIDE; Q9BTE1; -.
DR ProteomicsDB; 41095; -.
DR ProteomicsDB; 41730; -.
DR ProteomicsDB; 78981; -. [Q9BTE1-1]
DR Antibodypedia; 43088; 101 antibodies from 18 providers.
DR DNASU; 84516; -.
DR Ensembl; ENST00000300087.7; ENSP00000300087.2; ENSG00000166847.10. [Q9BTE1-1]
DR Ensembl; ENST00000563998.5; ENSP00000454691.1; ENSG00000166847.10. [Q9BTE1-2]
DR Ensembl; ENST00000568272.1; ENSP00000455685.1; ENSG00000166847.10. [Q9BTE1-3]
DR GeneID; 84516; -.
DR KEGG; hsa:84516; -.
DR MANE-Select; ENST00000300087.7; ENSP00000300087.2; NM_032486.4; NP_115875.1.
DR UCSC; uc002dly.3; human. [Q9BTE1-1]
DR CTD; 84516; -.
DR DisGeNET; 84516; -.
DR GeneCards; DCTN5; -.
DR HGNC; HGNC:24594; DCTN5.
DR HPA; ENSG00000166847; Low tissue specificity.
DR MIM; 612962; gene.
DR neXtProt; NX_Q9BTE1; -.
DR OpenTargets; ENSG00000166847; -.
DR PharmGKB; PA142672007; -.
DR VEuPathDB; HostDB:ENSG00000166847; -.
DR eggNOG; KOG3121; Eukaryota.
DR GeneTree; ENSGT00390000015360; -.
DR HOGENOM; CLU_088622_2_0_1; -.
DR InParanoid; Q9BTE1; -.
DR OMA; IAIGRYC; -.
DR OrthoDB; 1270774at2759; -.
DR PhylomeDB; Q9BTE1; -.
DR TreeFam; TF314194; -.
DR PathwayCommons; Q9BTE1; -.
DR Reactome; R-HSA-2132295; MHC class II antigen presentation.
DR Reactome; R-HSA-3371497; HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand.
DR Reactome; R-HSA-6807878; COPI-mediated anterograde transport.
DR Reactome; R-HSA-6811436; COPI-independent Golgi-to-ER retrograde traffic.
DR SignaLink; Q9BTE1; -.
DR BioGRID-ORCS; 84516; 742 hits in 1071 CRISPR screens.
DR ChiTaRS; DCTN5; human.
DR GeneWiki; DCTN5; -.
DR GenomeRNAi; 84516; -.
DR Pharos; Q9BTE1; Tbio.
DR PRO; PR:Q9BTE1; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q9BTE1; protein.
DR Bgee; ENSG00000166847; Expressed in islet of Langerhans and 199 other tissues.
DR ExpressionAtlas; Q9BTE1; baseline and differential.
DR Genevisible; Q9BTE1; HS.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005869; C:dynactin complex; IBA:GO_Central.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0031965; C:nuclear membrane; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0035904; P:aorta development; IEA:Ensembl.
DR GO; GO:0060976; P:coronary vasculature development; IEA:Ensembl.
DR GO; GO:0003281; P:ventricular septum development; IEA:Ensembl.
DR InterPro; IPR001451; Hexapep.
DR InterPro; IPR011004; Trimer_LpxA-like_sf.
DR Pfam; PF00132; Hexapep; 1.
DR SUPFAM; SSF51161; SSF51161; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Centromere; Chromosome;
KW Cytoplasm; Cytoskeleton; Kinetochore; Reference proteome.
FT CHAIN 1..182
FT /note="Dynactin subunit 5"
FT /id="PRO_0000079827"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895,
FT ECO:0007744|PubMed:22814378"
FT VAR_SEQ 40..182
FT /note="TIVMNDCIIRGDLANVRVGRHCVVKSRSVIRPPFKKFSKGVAFFPLHIGDHV
FT FIEEDCVVNAAQIGSYVHVGKNCVIGRRCVLKDCCKILDNTVLPPETVVPPFTVFSGCP
FT GLFSGELPECTQELMIDVTKSYYQKFLPLTQV -> NFVISVFLSPYIRCHSACRDRKR
FT SDESVRLSVNNREWWGLVNWRM (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_046697"
FT VAR_SEQ 151..182
FT /note="GLFSGELPECTQELMIDVTKSYYQKFLPLTQV -> AP (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_046023"
SQ SEQUENCE 182 AA; 20127 MW; 476DBD27A6CA1C35 CRC64;
MELGELLYNK SEYIETASGN KVSRQSVLCG SQNIVLNGKT IVMNDCIIRG DLANVRVGRH
CVVKSRSVIR PPFKKFSKGV AFFPLHIGDH VFIEEDCVVN AAQIGSYVHV GKNCVIGRRC
VLKDCCKILD NTVLPPETVV PPFTVFSGCP GLFSGELPEC TQELMIDVTK SYYQKFLPLT
QV
