DCTN6_HUMAN
ID DCTN6_HUMAN Reviewed; 190 AA.
AC O00399; B2RAC1;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Dynactin subunit 6;
DE AltName: Full=Dynactin subunit p27;
DE AltName: Full=Protein WS-3;
GN Name=DCTN6; Synonyms=WS3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Skin;
RX PubMed=9168138; DOI=10.1016/s0378-1119(96)00863-3;
RA Ichikawa K., Yamabe Y., Imamura O., Kuromitsu J., Sugawara K., Suzuki N.,
RA Shimamoto A., Matsumoto T., Tokutake Y., Kitao S., Kataoka H., Satoh M.,
RA Sugimoto M., Goto M., Sugawara M., Furuichi Y.;
RT "Cloning and characterization of a novel gene, WS-3, in human chromosome
RT 8p11-p12.";
RL Gene 189:277-287(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Substantia nigra;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), SUBUNIT, SUBCELLULAR LOCATION,
RP PHOSPHORYLATION AT THR-186, AND INTERACTION WITH PLK1.
RX PubMed=23455152; DOI=10.1038/emboj.2013.30;
RA Yeh T.Y., Kowalska A.K., Scipioni B.R., Cheong F.K., Zheng M.,
RA Derewenda U., Derewenda Z.S., Schroer T.A.;
RT "Dynactin helps target Polo-like kinase 1 to kinetochores via its left-
RT handed beta-helical p27 subunit.";
RL EMBO J. 32:1023-1035(2013).
CC -!- SUBUNIT: Member of the pointed-end complex of the dynactin shoulder
CC complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10.
CC Within the complex DCTN6 forms a heterodimer with DCTN5. Interacts with
CC PLK1. {ECO:0000269|PubMed:23455152}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:23455152}.
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- PTM: Phosphorylation at Thr-186 by CDK1 during mitotic prometaphase
CC creates a binding site for PLK1 that facilitates its recruitment to
CC kinetochores. {ECO:0000269|PubMed:23455152}.
CC -!- SIMILARITY: Belongs to the dynactin subunits 5/6 family. Dynactin
CC subunit 6 subfamily. {ECO:0000305}.
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DR EMBL; D84145; BAA19984.1; -; mRNA.
DR EMBL; CR456851; CAG33132.1; -; mRNA.
DR EMBL; AK314128; BAG36818.1; -; mRNA.
DR EMBL; CH471080; EAW63466.1; -; Genomic_DNA.
DR EMBL; BC013175; AAH13175.1; -; mRNA.
DR CCDS; CCDS6076.1; -.
DR RefSeq; NP_006562.1; NM_006571.3.
DR PDB; 3TV0; X-ray; 2.15 A; A/B=1-190.
DR PDBsum; 3TV0; -.
DR AlphaFoldDB; O00399; -.
DR SMR; O00399; -.
DR BioGRID; 115913; 72.
DR IntAct; O00399; 43.
DR MINT; O00399; -.
DR STRING; 9606.ENSP00000221114; -.
DR iPTMnet; O00399; -.
DR MetOSite; O00399; -.
DR PhosphoSitePlus; O00399; -.
DR BioMuta; DCTN6; -.
DR EPD; O00399; -.
DR jPOST; O00399; -.
DR MassIVE; O00399; -.
DR MaxQB; O00399; -.
DR PaxDb; O00399; -.
DR PeptideAtlas; O00399; -.
DR PRIDE; O00399; -.
DR ProteomicsDB; 47865; -.
DR Antibodypedia; 10535; 174 antibodies from 25 providers.
DR DNASU; 10671; -.
DR Ensembl; ENST00000221114.8; ENSP00000221114.3; ENSG00000104671.8.
DR GeneID; 10671; -.
DR KEGG; hsa:10671; -.
DR MANE-Select; ENST00000221114.8; ENSP00000221114.3; NM_006571.4; NP_006562.1.
DR UCSC; uc003xhy.4; human.
DR CTD; 10671; -.
DR DisGeNET; 10671; -.
DR GeneCards; DCTN6; -.
DR HGNC; HGNC:16964; DCTN6.
DR HPA; ENSG00000104671; Low tissue specificity.
DR MIM; 612963; gene.
DR neXtProt; NX_O00399; -.
DR OpenTargets; ENSG00000104671; -.
DR PharmGKB; PA134927975; -.
DR VEuPathDB; HostDB:ENSG00000104671; -.
DR eggNOG; KOG4042; Eukaryota.
DR GeneTree; ENSGT00390000017890; -.
DR HOGENOM; CLU_085418_1_0_1; -.
DR InParanoid; O00399; -.
DR OMA; PDYTVVY; -.
DR OrthoDB; 1278795at2759; -.
DR PhylomeDB; O00399; -.
DR TreeFam; TF352888; -.
DR PathwayCommons; O00399; -.
DR Reactome; R-HSA-2132295; MHC class II antigen presentation.
DR Reactome; R-HSA-3371497; HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand.
DR Reactome; R-HSA-6807878; COPI-mediated anterograde transport.
DR Reactome; R-HSA-6811436; COPI-independent Golgi-to-ER retrograde traffic.
DR SignaLink; O00399; -.
DR SIGNOR; O00399; -.
DR BioGRID-ORCS; 10671; 658 hits in 1083 CRISPR screens.
DR ChiTaRS; DCTN6; human.
DR GenomeRNAi; 10671; -.
DR Pharos; O00399; Tdark.
DR PRO; PR:O00399; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; O00399; protein.
DR Bgee; ENSG00000104671; Expressed in myocardium and 213 other tissues.
DR ExpressionAtlas; O00399; baseline and differential.
DR Genevisible; O00399; HS.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005869; C:dynactin complex; IBA:GO_Central.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0070840; F:dynein complex binding; IBA:GO_Central.
DR GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR InterPro; IPR027777; DCTN6.
DR InterPro; IPR001451; Hexapep.
DR InterPro; IPR011004; Trimer_LpxA-like_sf.
DR PANTHER; PTHR13072; PTHR13072; 1.
DR Pfam; PF00132; Hexapep; 1.
DR SUPFAM; SSF51161; SSF51161; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Centromere; Chromosome; Cytoplasm; Cytoskeleton; Kinetochore;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..190
FT /note="Dynactin subunit 6"
FT /id="PRO_0000079830"
FT MOD_RES 186
FT /note="Phosphothreonine; by CDK1"
FT /evidence="ECO:0000269|PubMed:23455152"
FT STRAND 9..11
FT /evidence="ECO:0007829|PDB:3TV0"
FT STRAND 21..29
FT /evidence="ECO:0007829|PDB:3TV0"
FT STRAND 40..46
FT /evidence="ECO:0007829|PDB:3TV0"
FT STRAND 48..50
FT /evidence="ECO:0007829|PDB:3TV0"
FT STRAND 61..64
FT /evidence="ECO:0007829|PDB:3TV0"
FT STRAND 82..84
FT /evidence="ECO:0007829|PDB:3TV0"
FT STRAND 99..101
FT /evidence="ECO:0007829|PDB:3TV0"
FT STRAND 117..119
FT /evidence="ECO:0007829|PDB:3TV0"
FT STRAND 135..137
FT /evidence="ECO:0007829|PDB:3TV0"
FT STRAND 141..145
FT /evidence="ECO:0007829|PDB:3TV0"
FT STRAND 150..153
FT /evidence="ECO:0007829|PDB:3TV0"
SQ SEQUENCE 190 AA; 20747 MW; 7D0B12C0029C77B7 CRC64;
MAEKTQKSVK IAPGAVVCVE SEIRGDVTIG PRTVIHPKAR IIAEAGPIVI GEGNLIEEQA
LIINAYPDNI TPDTEDPEPK PMIIGTNNVF EVGCYSQAMK MGDNNVIESK AYVGRNVILT
SGCIIGACCN LNTFEVIPEN TVIYGADCLR RVQTERPQPQ TLQLDFLMKI LPNYHHLKKT
MKGSSTPVKN
