ID   DCTP_PSEAE              Reviewed;         331 AA.
AC   Q9HU18;
DT   20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=C4-dicarboxylate-binding periplasmic protein DctP {ECO:0000305};
DE   Flags: Precursor;
GN   Name=dctP {ECO:0000303|PubMed:21725012};
GN   OrderedLocusNames=PA5167 {ECO:0000312|EMBL:AAG08552.1};
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, SUBUNIT, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=21725012; DOI=10.1128/jb.05074-11;
RA   Valentini M., Storelli N., Lapouge K.;
RT   "Identification of C(4)-dicarboxylate transport systems in Pseudomonas
RT   aeruginosa PAO1.";
RL   J. Bacteriol. 193:4307-4316(2011).
CC   -!- FUNCTION: Part of the tripartite ATP-independent periplasmic (TRAP)
CC       transport system DctPQM involved in C4-dicarboxylates uptake.
CC       {ECO:0000269|PubMed:21725012}.
CC   -!- SUBUNIT: The complex comprises the extracytoplasmic solute receptor
CC       protein DctP, and the two transmembrane proteins DctQ and DctM.
CC       {ECO:0000305|PubMed:21725012}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250|UniProtKB:P37735}.
CC   -!- INDUCTION: Expression is maximal in early exponential growth phase and
CC       declines with cell density to reach a plateau in the stationary growth
CC       phase. Induced by the C(4)-dicarboxylates succinate, fumarate and
CC       malate. Positively regulated by RpoN and the DctB/DctD two-component
CC       system. Negatively regulated by DctA. {ECO:0000269|PubMed:21725012}.
CC   -!- DISRUPTION PHENOTYPE: The dctA-dctPQM double mutant shows no growth on
CC       malate and fumarate and residual growth on succinate.
CC       {ECO:0000269|PubMed:21725012}.
CC   -!- MISCELLANEOUS: The DctPQM carrier is more efficient than the DctA
CC       carrier for the utilization of succinate at micromolar concentrations,
CC       whereas DctA is the major transporter at millimolar concentrations.
CC       {ECO:0000269|PubMed:21725012}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 7 family.
CC       {ECO:0000305}.
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DR   EMBL; AE004091; AAG08552.1; -; Genomic_DNA.
DR   PIR; H83000; H83000.
DR   RefSeq; NP_253854.1; NC_002516.2.
DR   RefSeq; WP_003105525.1; NZ_QZGE01000002.1.
DR   AlphaFoldDB; Q9HU18; -.
DR   SMR; Q9HU18; -.
DR   STRING; 287.DR97_2535; -.
DR   PaxDb; Q9HU18; -.
DR   PRIDE; Q9HU18; -.
DR   DNASU; 879964; -.
DR   EnsemblBacteria; AAG08552; AAG08552; PA5167.
DR   GeneID; 879964; -.
DR   KEGG; pae:PA5167; -.
DR   PATRIC; fig|208964.12.peg.5415; -.
DR   PseudoCAP; PA5167; -.
DR   HOGENOM; CLU_036176_1_3_6; -.
DR   InParanoid; Q9HU18; -.
DR   OMA; AFWHNGM; -.
DR   PhylomeDB; Q9HU18; -.
DR   BioCyc; PAER208964:G1FZ6-5284-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:InterPro.
DR   GO; GO:0015740; P:C4-dicarboxylate transport; IMP:PseudoCAP.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   Gene3D; 3.40.190.170; -; 1.
DR   InterPro; IPR018389; DctP_fam.
DR   InterPro; IPR004682; TRAP_DctP.
DR   InterPro; IPR038404; TRAP_DctP_sf.
DR   PANTHER; PTHR33376; PTHR33376; 1.
DR   Pfam; PF03480; DctP; 1.
DR   PIRSF; PIRSF006470; DctB; 1.
DR   TIGRFAMs; TIGR00787; dctP; 1.
PE   1: Evidence at protein level;
KW   Periplasm; Reference proteome; Signal; Transport.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..331
FT                   /note="C4-dicarboxylate-binding periplasmic protein DctP"
FT                   /id="PRO_5004326794"
SQ   SEQUENCE   331 AA;  37051 MW;  346F1C169F6134CD CRC64;
     MLKHTAKALV CALSLTVAGI VQAADPIVIK FSHVVAEHTP KGQGALLFKK LVEERLPGKV
     KVEVYPNSSL FGDGKEMEAL LLGDVQIIAP SLAKFEQYTK KLQIFDLPFL FDNIQAVDRF
     QQSPQGKELL TSMQDKGITG LGYWHNGMKQ LSANKPLREP KDARGLKFRV QASKVLEEQF
     KAVRANPRKM SFAEVYQGLQ TGVVNGTENP WSNIYSQKMH EVQKYITESD HGVLDYMVIT
     NTKFWNGLPE DVRGVLAKTM DEVTVEVNKQ AEALNQGDKQ RIVEAKTSEI IELTPEQRAE
     WRKAMQPVWK KFEGEIGADL IKAAEAANQA Q